Quantitation of glutaminase mRNA in enterocytes using competitive RT-PCR

Molecular and Cellular Probes

Volumn 12, Issue 5, 1998, Pages 339-341

  Kong, S E ^a   Heel, K A ^a   Hall, J C ^a   McCauley, R D ^a  

^a ROYAL PERTH HOSPITAL   (Australia)

Author keywords

Competitive RT PCR; Glutaminase; mRNA

Indexed keywords

COMPLEMENTARY DNA; GLUTAMINASE; MESSENGER RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ENZYME ACTIVITY; GENE AMPLIFICATION; HOUSEKEEPING GENE; INTESTINE CELL; INTESTINE MUCOSA; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 0032191161     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1998.0183     Document Type: Article

References (12)

1. 1. Hall, J. C., Heel, K. A. & McCauley, R. (1996). Glutamine. British Journal of Surgery 83, 305-12.
2. 2. Weiser, M. M. (1973). Intestinal epithelial cell surface membrane glycoprotein synthesis. Journal of Biological Chemistry 248, 2536-41.
3. 3. Mansur, N. R., Meyer-Siegler, K., Wurzer, J. C. & Sirover, M. A. (1993). Cell cycle regulation of the glyceraldehyde-3-phosphate dehydrogenase/uracil DNA glycosylase gene in normal human cells. Nucleic Acids Research 21, 993-8.
4. 4. Weiser, M. M., Sykes, D. E. & Killen, P. D. (1990). Rat intestinal basement membrane synthesis. Epithelial versus nonepithelial contributions. Laboratory Investigation 62, 325-30.
5. 5. Yamada, H., Chen, D., Monstein, H. J. & Hakanson, R. (1997). Effects of fasting on the expression of gastrin, cholecystokinin, and somatostatin genes and of various housekeeping genes in the pancreas and upper digestive tract of rats. Biochemical and Biophysical Research Communications 231, 835-8.
6. 6. Siebert, P. D. & Larrick, J. W. (1992). Competitive PCR. Nature 83, 557-8.
7. 7. Sellner, L. N. & Turbett, G. R. (1996). The presence of a pseudogene may affect the use of HPRT as an endogenous mRNA control in RT-PCR. Molecular and Cellular Probes 10, 481-3.
8. 8. Banner, C., Hwang, J.-J., Shapiro, R. A. et al. (1988). Isolation of a cDNA for rat brain glutaminase. Brain Research 427, 247-54.
9. 9. Celi, F. S., Zenilman, M. E. & Shuldiner, A. R. (1993). A rapid and versatile method to synthesize internal standards for competitive PCR. Nucleic Acids Research 21, 1047.
10. 10. Frohman, M. A. (1994). Cloning PCR products. In The Polymerase Chain Reaction (Mullis, K. B., Ferré, F. & Gibbs, R. A., eds) pp. 14-37. Boston: Birkhäuser Boston.
11. 11. Siebert, P. D. & Kellogg, D. E. (1995). PCR MIMICs: competitive DNA fragments for use in quantitative PCR. In PCR 2 A Practical Approach (McPherson, M. J., Hames, B. D. & Taylor, G. R., eds) pp. 135-48. Oxford: Oxford University Press.
12. 12. Curthoys, N. P. & Lowry, O. H. (1973). The distribution of glutaminase isoenzymes in the various structures of the nephron in normal, acidotic, and alkalotic rat kidney. Journal of Biological Chemistry 248, 162-8.