Yeast cell-surface display - Applications of molecular display

Applied Microbiology and Biotechnology

Volumn 64, Issue 1, 2004, Pages 28-40

  Kondo, A ^a   Ueda, M ^b  

^a KOBE UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL ENZYME; FUNGAL PROTEIN; FUNGUS ANTIBODY; PEPTIDE DERIVATIVE;

MICROBIOLOGY; YEAST;

BIOCATALYST; BIOENGINEERING; BIOTECHNOLOGY; CATALYST; CELL SURFACE; COMBINATORIAL CHEMISTRY; COMBINATORIAL LIBRARY; DISPLAY SYSTEM; FUNGAL GENETICS; MOLECULAR SIZE; NONHUMAN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SEPARATION TECHNIQUE; SHORT SURVEY; SURFACE PROPERTY; TECHNIQUE; YEAST CELL;

AGGLUTININS; ANTIBODIES; ANTIGENS; BIOTECHNOLOGY; CELL WALL; ENZYMES; GENETIC ENGINEERING; GLYCOSYLPHOSPHATIDYLINOSITOLS; MEMBRANE PROTEINS; PEPTIDE LIBRARY; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

FUNGI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 1642340053     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1492-3     Document Type: Short Survey

References (106)

1. Anonymous (1997) Arming yeast with cell-surface catalysts. Chem Eng News 75:32
2. Ballou CE (1982) The molecular biology of the yeast Saccharomyces. In: Strathern, JN, Jones EW, Broach JR (eds) Metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 335-360
3. Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol 15:553-557
4. Boder ET, Midelfort SK, Wittrup KD (2000) Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc Natl Acad Sci USA 97:10701-10705
5. Bony M, Thines-Sempoux D, Barre P, Blondin B (1997) Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p. J. Bacteriol. 179:4929-4936
6. Bossier P, Goethals P, Rodrigues-Pousada C (1997) Constitutive flocculation in Saccharomyces cerevisiae through overexpression of the GTS1 gene, coding for a 'Glo'-type Zn-finger-containing protein. Yeast 13:717-725
7. Cappellaro C, Hauser K, Mrsa V, Watzele M, Watzele G, Gruber C, Tanner W (1991) Saccharomyces cerevisiae a-and α-agglutinin: characterization of their molecular interaction. EMBO J 10:4081-4088
8. Chiswell DJ, McCafferty J (1992) Phage antibodies: will new 'coliclonal' antibodies replace monoclonal antibodies? Trends Biotechnol 10:80-84
9. Cid VJ, Duran A, del Rey F, Snyder M P, Nombela C, Sanchez M (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59:345-386
10. Cross GAM (1990) Cellular and genetic aspects of antigenic variation in rypanosomes. Annu Rev Immunol 8:83-110
11. Dustin ML, Selvaraj P, Mattaliano RJ, Springer T A (1987) Anchoring mechanisms for LFA-3 cell adhesion glycoprotein at membrane surface. Nature 329:846-848
12. Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JMW, Yeung YA, Cochran JR, Heinzelman P, Colby D, Swers J, Graff C, Wiley HS, Wittrup KD (2003) Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol 21:163-170
13. Fleet GH (1991) Cell walls. In Rose AH, Harrison JS (eds), The yeasts, 2nd edn, vol 4. Yeast organelles. Academic, London, pp 199-277
14. Fleet GH, Manners DJ (1977) The enzymic degradation of an alkali-soluble glucan from the cell walls of Saccharomyces cerevisiae. J Gen Microbiol 98:315-327
15. Francisco JA, Earhart CF, Georgiou G (1992) Transport and anchoring of β-lactamase to the external surface of Escherichia coli. Proc Natl Acad. Sci USA 89:2713-2717
16. Francisco JA, Stathopoulos C, Warren RAJ, Kilburn DG, Georgiou G (1993) Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Biol/ Technol 11:491-495
17. Fredette B, Rutishauer U, Landmesser L (1993) Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development. J Cell Biol 123:1867-1888
18. Fujita Y, Katahira S, Ueda, M, Tanaka, A, Okada, H, Morikawa, Y, Fukuda, H, Kondo A (2002a) Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae, J Mol Catal B 17:189-195
19. Fujita Y, Takahashi S, Ueda M, Tanaka A, Okada H, Morikawa Y, Kawaguchi T, Arai M, Fukuda H, Kondo A (2002b) Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl Environ Microbiol 68:5136-5141
20. Furukawa H, Shimojyo R, Ohnishi N, Fukuda H, Kondo A (2003) Affinity selection of target cells from cell surface display libraries: a novel procedure using thermo-responsive magnetic nanoparticles. Appl Microbiol Biotechnol 62:478-483
21. Georgiou G, Poetschke HL, Stathopoulos C, Francisco JA (1993) Practical applications of engineering gram-negative bacterial cell surfaces. Trends Biotechnol 11:6-10
22. Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss III R (1997) Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15:29-34
23. Gunneriusson E, Samuelson M, Uhlen M, Nygren P-A, Stahl S (1996) Surface display of a functional single-chain Fv antibody on Staphylococci. J Bacteriol 178:1341-1346
24. Harashima S, Mizuno T, Mabuchi H, Yoshimitsu S, Ramesh R, Hasebe M, Tanaka A, Oshima Y (1995) Mutations causing high basal level transcription that is independent of transcriptional activators but dependent on choromosomal position in Saccharomyces cerevisiae. Mol Gen Genet 247:716
25. Hardwick KG, Boothroyd JC, Rudner AD, Pelham HRB (1992) Genes that allow yeast cells to grow in the absence of the HDEL receptor. EMBO J 11:4187-4195
26. Harrison JL, Taylor IM, O'Connor CD (1990) Presentation of foreign antigenic determinants at the bacterial cell surface using the TraT lipoprotein. Res Microbiol 141:1009-1012
27. Hedegaard L, Klemm P (1989) Type 1 fimbriae of Escherichia coli as carrier of heterologous antigenic sequences. Gene 85:115-124
28. Homans SW, Ferguson MA, Dwek RA, Rademacher TW, Anand R, Williams AF (1988) Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein. Nature 333:269-272
29. Horisberger M, Vonlanthen M (1977) Location of mannan and chitin on thin sections of budding yeasts with gold markers. Arch Microbiol 115:1-7
30. Jensen TK, Toppari J, Keiding N, Skakkebaek NE (1995) Do environmental oestrogens contribute to the decline in male reproductive health? Clin Chem 41:1896-1931
31. Kanai T, Atomi H, Umemura K, Ueno H, Teranishi Y, Ueda M, Tanaka A (1996) A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis. Appl Microbiol Biotechnol 44:759-765
32. Kapteyn JC, Montijn RC, Vink E, de la Crua J, Llobell A, Douwes JE, Shimoi H, Lipke PN, Klis FM (1996) Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/β-1,6-glucan heteropolymer. Glycobiology 6:337-345
33. Keike MC, Cho BK, Boder ET, Kranz DM, Wittrup KD (1997) Isolation of anti-T cell receptor scFv mutants by yeast surface display. Protein Eng 10:1303-1310
34. Keike MC, Shusta EV, Boder ET, Teyto, L, Wittrup K, Kranz DM (1999) Selection of functional T cell receptor mutants from a yeast surface-display library. Proc Natl Acad Sci USA 96:5651-5656
35. Kemper MA, Urrutia MM, Beveridge TJ, Koch AL, Doyle RJ (1993) Proton motive force may regulate cell wall-associated enzymes of Bacillus subtills. J Bacteriol 175:5690-5696
36. Klis FM (1994) Cell wall assembly in yeast. Yeast 10:851-869
37. Kondo A, Shigechi H, Abe M, Uyama K, Matsumoto T, Takahashi S, Ueda M, Tanaka A, Kishimoto M, Fukuda H (2002) High level production from starch by a flocculent Saccharomyces cerevisiae strain displaying cell surface glucoamylase. Appl Microbiol Biotechnol 58:291-296
38. Kotrba P, Doleckova L, De Lorenzo V, Ruml T (1999) Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol 65:1092-1098
39. Kugimiya W, Otani Y, Kohno M, Hashimoto Y (1992) Cloning and sequence analysis of cDNA encoding Rhizopus niveus lipase. Biosci Biotech Biochem 56:716-719
40. Kuroda K, Shibasaki S, Ueda M, Tanaka A (2001) Cell surface-engineered yeast displaying histidine oligopeptide (hexa-His) has enhanced adsorption of and tolerance to heavy metal ions. Appl Microbiol Biotechnol 57:697-701
41. Kuroda K, Ueda M, Shibasaki S, Tanaka A (2002) Cell-surface-engineered yeast with ability to bind, and self-sggregate in response to, copper ion. Appl Microbiol Biotechnol 59:259-264
42. Lehtiö J, Wernérus H, Samuelson P, Teeri TT, Ståhl S (2001) Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain. FEMS Microbiol Lett 195:197-204
43. Lin Y, Tsumuraya T, Wakabayashi T, Shiraga S. Fujii I, Kondo A, Tanaka A, Ueda M (2003) Display of functional heterooligomeric catalytic antibody on the yeast cell surface. Appl Microbiol Biotechnol 62:226-232
44. Lipke PN, Kurjan J (1992) Sexual agglutination in budding yeasts: structure, function, and regulation of adhesion glycoproteins. Microbiol Rev 56:180-194
45. Lipke PN, Wojciechowicz D, Kurjan J (1989) AGα1 is the structural gene for the Saccharomyces cerevisiae α-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol Cell Biol 9:3155-3165
46. Little M, Fuchs P, Breitling F, Dubel S (1993) Bacterial surface presentation of proteins and peptides: An alternative to phage technology? Trends Biotechnol 11:3-5
47. Lorenz MC, Heitman J (1998) The MEP2 ammonium permease regulates pseudohyphal defferentiation in Saccharomyces cerevisiae. EMBO J 17:1236-1247
48. Lu CF, Kurjan J, Lipke PN (1994) A pathway for cell anchorage of Saccharomyces cerevisiae α-agglutinin. Mol Cell Biol 14:4825-4833
49. Lu CF, Montijin RC, Brown JL, Klis FM, Kurjan J, Bussey H, Lipke PN (1995) Glycosylphosphatidylinositol-dependent cross linking of α-agglutinin and β 1, 6-glucan in the Saccharomyces cerevisiae cell wall. J Cell Biol 128:333-340
50. Manners DJ, Masson AJ, Patterson JC (1973a) The structure of a β-(1,3)-D-glucan from yeast cell walls. Biochem J 135:19-30
51. Manners DJ, Masson AJ, Patterson JC (1973b) The structure of a β-(1,6)-D-glucan from yeast cell walls. Biochem J 135:31-36
52. Marini AM, Vissers S, Urrestarazu A, Andre B (1994) Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae. EMBO J 13:3456-3463
53. Marini AM, Soussi-Boudekou S, Vissers S, Andre B (1997) A family of ammonium transporters in Saccharomyces cerevisiae. Mol Cell Biol 17:4282-4293
54. Matsumoto T, Fukuda H, Ueda M, Tanaka A, Kondo A (2002) Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. Appl Environ Microbiol 68:4517-4522
55. Matsumoto T, Ito M, Fukuda H, Kondo A (2004) Enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. Appl Microbiol Biotechnol, in press
56. Meyhack B, Bajwa W, Rudolph H, Hinnen A (1982) Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences. EMBO J 1:675-680
57. Miki BL, Poon NH, James AP, Seligy VL (1982) Possible mechanism for flocculation interactions governed by gene FLO1 in Saccharomyces cerevisiae. J Bacteriol 150:878-889
58. Mitsui K, Yaguchi S, Tsurugi K (1994) The GTS1 gene, which contains a Gly-Thr repeat, affects the timing of budding and cell size of the yeast Saccharomyces cerevisiae. Mol Cell Biol 14:5569-5578
59. Miura S, Zou W, Ueda M, Tanaka A (2000) Screening of genes involved in isooctane-tolerance in Saccharomyces cerevisiae using mRNA differential display. Appl Environ Microbiol 66:4883-4889
60. Miyashita H, Hara T, Tanimura R, Fukuyama S, Cagnon C, Kohara A, Fujii I (1997) Site-directed mutagenesis of active site contact residues in a hydrolytic abzyme: evidence for an essential histidine involved in transition state stabilization. J Mol Biol 267:1247-1257
61. Murai T, Ueda M, Yamamura M, Atomi H, Shibasaki Y, Kamasawa N, Osumi M, Amachi T, Tanaka A (1997a) Construction of a starch-utilizing yeast by cell surface engineering. Appl Environ Microbiol 63:1362-1366
62. Murai T, Ueda M, Atomi H, Shibasaki Y, Kamasawa N, Osumi M, Kawaguchi T, Arai M Tanaka A (1997b) Genetic immobilization of cellulase on the cell surface of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 48:499-503
63. Murai T, Ueda M, Kawaguchi T, Arai M, Tanaka A (1998) Assimilation of cellulosic materials by cell surface-engineered yeast displaying β-glucosidase and carboxymethylcellulase from Aspergillus aculeatus. Appl Environ Microbiol 64:4857-4861
64. Murai T, Ueda M, Shibasaki Y, Kamasawa N, Osumi M, Imanaka T, Tanaka A (1999) Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface. Appl Microbiol Biotechnol 51:65-70
65. Nakamura Y, Shibasaki S, Ueda M, Tanaka A, Fukuda H, Kondo A (2001) Development of novel whole-cell immunoadsorbents by yeast surface display of the IgG-binding domain. Appl Microbiol Biotechnol 57:500-505
66. Nam J, Fujita Y, Arai T, Kondo A, Morikaw Y, Okada H, Ueda M, Tanaka A (2002) Construction of engineered yeast with the ability of binding to cellulose. J Mol Catalys 17:197-202
67. Newton SMC, Jacob CO, Stocker BAD (1989) Immune response to cholera toxin epitope inserted in salmonella flagellin. Science 244:70-72
68. Nilsson, B, Moks, T, Jansson, B, Abrahmsén, L, Elmbrad, A, Holmgren, E, Henrichson, C, Jones, TA, Uhlén, M (1987) A synthetic IgG-binding domain based on Staphylococcal protein A. Prot Eng 1:107-113
69. Nishikawa J, Saito K, Goto J, Dakeyama F, Matsuo M, Nishihara T (1999) New screening methods for chemicals with hormonal activities using interaction of nuclear hormone receptor with coactivator. Toxicol Appl Pharmacol 154:76-83
70. Routledge EJ, Sumpter JP (1997) Structural features of alkylphenolic chemicals associated with estrogenic activity. J Biol Chem 272:3280-3288
71. Roy A, Lu CF, Marykwas DL, Lipke PN, Kurjan J (1991) The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein α-agglutinin. Mol Cell Biol 11:4196-4206
72. Rudolph H, Hinnen A (1987) The yeast PHO5 promoter: Phosphate-control elements and sequences mediating mRNA start-site selection. Proc Natl Acad Sci USA 84:1340-1344
73. Samuelson P, Hansson M, Ahborg N, Andreoni C, Gotz F, Bachi T, Nguyen TN, Binz H, Uhlen M, Stahl S (1995) Cell surface display of recombinant proteins on Staphylococcus carnosus. J Bacteriol 177:1470-1476
74. Samuelson P, Wernerus H, Svedberg M, Stahl S (2000) Staphylococcal surface display of metal-binding polyhistidyl peptides. Appl Environ Microbiol 66:1243-1248
75. Sato N, Matsumoto T, Ueda M, Tanaka A, Fukuda H, Kondo A (2002) Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates, Appl Microbiol Biotechnol 60:469-474
76. Schekman R (1992) Genetic and biochemical analysis of vesicular traffic in yeast. Curr Opin Cell Biol 4:587-592
77. Schekman R, Novick P (1982) The molecular biology of the yeast Saccharomyces,. In: Strathern JN, Jones EW, Broach JR (eds) Metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 361-398
78. Schneewind O, Fowler A, Faull K F (1995) Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science 269:103-106
79. Schreuder MP, Brekelmans S, Van den Ende H, Klis FM (1993) Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Yeast 9:399-409
80. Schreuder MP, Mooren ATA, Toschka HY, Verrips CT, Klis FM (1996) Immobilizing proteins on the yeast cells. Trends Biotechnol 14:115-120
81. Scott JK, Smith GP (1990) Searching for peptide ligands with an epitope library. Science 249:386-390
82. Sharpe RM (1998) Environmental oestrogens and male infertility. Pure Appl Chem 70:1685-1701
83. Shibasaki S, Ueda M, Iizuka T, Hirayama M, Ikeda Y, Kamasawa M, Osumi M, Tanaka A (2001a) Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by the fluorometric and confocal laser scanning microscopic analyses. Appl Microbiol Biotechnol 55:471-475
84. Shibasaki S, Ueda M, Ye K, Shimizu K, Kamasawa N, Osumi M, Tanaka A (2001b) Creation of cell surface-engineered yeast which can emit different fluorescence in response to the glucose concentration. Appl Microbiol Biotechnol 57:528-533
85. Shibasaki S, Ninomiya Y, Ueda M, Iwahashi M, Katsuragi T, Tani Y, Harashima S, Tanaka A (2001c) Intelligent yeast strain with the ability of self-monitoring of the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface. Appl Miocrobiol Biotechnol 57:702-707
86. Shigechi H, Uyama K, Matsumoto T, Ueda M, Tanaka A, Fukuda H, Kondo A (2002) Efficent ethanol production from through development of novel flocculent yeast strains displaying glucoamylase and co-displying of secreting α-amylase. J Mol Cat B 17:179-187
87. Shimojo R, Furukawa H, Fukuda H, Kondo A (2003) Preparation of yeast strains displaying IgG binding domain ZZ and EGFP for novel antigen detection system. J Biosci Bioeng 96:493-495
88. Shusta EV, Holler PD, Kieke MC, Kranz DM, Wittrup KD (2000) Directed evolution of a scafford for T-cell receptor engineering. Nat Biotechnol 18:754-759
89. Sohoni P, Sumpter JP (1998) Several environmental oestrogens are also anti-androgens. J Endocrinol 158:327-339
90. Sousa C, Cebola A, de Lorenzo V (1996) Enhanced metallosorption of bacterial cells displaying poly-His peptides. Nat Biotechnol 14:1017-1020
91. Terrance K, Heller P, Wu Y-S, Lipke PN (1987) Identification of glycoprotein components of α-agglutinin, a cell adhesion protein from Saccharomyces cerevisiae. J Bacteriol 169:475-482
92. Teunissen AW, Holub E, van der Hucht J, van den Berg JA, Steensma HY (1993) Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae. Yeast 9:423-427
93. Ueda M, Tanaka A (2000a) Genetic immobilization of proteins on the yeast cell surface. Biotechnol Adv 18:121-140
94. Ueda M, Tanaka A (2000b) Cell surface engineering of yeast - construction of arming yeast with biocatalytst. J Biosci Bioeng 90:125-136
95. Valentin E, Herrero E, Pastor FIJ, Sentandreu R (1984) Solubilization and analysis of mannoprotein molecules from the cell wall of Saccharomyces cerevisiae. J Gen Microbiol 130:1419-1428
96. Van der Vaart JM, Caro LHP, Chapman JW, Klis FM, Verrips CT (1995) Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. J Bacteriol 177:3104-3110
97. Wagner J-C, Escher C, Wolf DH (1987) Some characteristics of hormone (pheromone) processing enzymes in yeast. FEBS Lett 218:31-34
98. Wang AA, Mulchandani A, Chen W (2001) Whole-cell immobilization using cell surface-exposed cellulose-binding domain. Biotechnol Prog 17:407-411
99. Washida M, Takahashi S, Ueda M, Tanaka A (2001) Spacer-mediated display of active lipase on the yeast cell surface. Appl Microbiol Biotechnol 56:681-686
100. Watari J, Takata Y, Ogawa M, Sahara H, Koshino M, Onnela M-L, Airaksinen U, Jaatinen R, Penttila M, Keranen S (1994) Molecular cloning and analysis of the yeast flocculation gene FLO1. Yeast 10:211-225
101. Watzele M, Klis FM, Tanner W (1988) Purification and characterization of the inducible a-agglutinin of Saccharomyces cerevisiae. EMBO J 7:1483-1488
102. Yaguchi S, Mitsui K, Iha H, Tsurugi K (2000) Phosphorylation of the GTS1 gene product of the yeast Saccharomyces cerevisiae and its effect on heat tolerance and flocculation. FEMS Microbiol Lett 187:179-84
103. Yasui M, Shibasaki S, Kuroda K, Ueda M, Kawada N, Nishikawa J, Nishihara T, Tanaka A (2002) An arming yeast with the ability to entrap fluorescent 17β-estradiol on the cell surface. Appl Microbiol Biotechnol 59:329-331
104. Ye K, Shibasaki S, Ueda M, Tanaka A (2000) Construction of engineered yeast with glucose-inducible emission of green fluorescence from cell surface. Appl Microbiol Biotechnol 54:90-96
105. Zou W, Ueda M, Yamanaka H, Tanaka A (2001) Construction of a combinatorial protein library displayed on yeast. J Biosci Biotechnol 92:393-396
106. Zou W, Ueda M, Tanaka A (2002) Screening of a molecule endowing Saccharomyces cerevisiae with n-nonane-tolerance from a combinatorial random protein library. Appl Microbiol Biotechnol 58:806-812