Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing β-glucosidase and carboxymethylcellulase from Aspergillus aculeatus

Applied and Environmental Microbiology

Volumn 64, Issue 12, 1998, Pages 4857-4861

  Murai, Toshiyuki ^a   Ueda, Mitsuyoshi ^a   Kawaguchi, Takashi ^b   Arai, Motoo ^b   Tanaka, Atsuo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLUCOSIDASE; CELLULASE; CELLULOSE; OLIGOSACCHARIDE;

ARTICLE; ASPERGILLUS; CARBOHYDRATE SYNTHESIS; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; DEGRADATION; ENZYME ASSAY; FUNGUS GROWTH; GENE TARGETING; GENETIC MANIPULATION; HYDROLYSIS; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION;

ASPERGILLUS; ASPERGILLUS ACULEATUS; FUNGI; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

EID: 0031767473     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.12.4857-4861.1998     Document Type: Article

References (29)

1. Adam, A. C., M. Rubio-Texeira, and J. Polaina. 1995. Induced expression of bacterial β-glucosidase activity in Saccharomyces. Yeast 11:395-406.
2. 1a. Anonymous. 1997. Arming yeast with cell-surface catalysts. Chem. Eng. News 75:32.
3. Ashikari, T., N. Kiuchi-Goto, Y. Tanaka, Y. Shibano, T. Amachi, and H. Yoshizumi. 1989. High expression and efficient secretion of Rhizopus oryzae glucoamylase in the yeast Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 30:515-520.
4. Barnett, J. A. 1976. The utilization of sugars by yeasts. Adv. Carbohydr. Chem. Biochem. 32:125-234.
5. Borooah, J., D. H. Leaback, and P. G. Walker. 1961. Studies on glucosaminidase. 2. Substrates for N-acetyl-β-glucosaminidase. Biochem. J. 78:106-110.
6. Cummings, C., and T. Fowler. 1996. Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae. Curr. Genet. 29:227-233.
7. Kawaguchi, T., T. Enoki, S. Tsurumaki, J. Sumitani, M. Ueda, T. Ooi, and M. Arai. 1996. Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus. Gene 173:287-288.
8. Kobori, H., M. Sato, and M. Osumi. 1992. Relationship of actin organization to growth in the two forms of the dimorphic yeast Candida tropicalis. Protoplasma 167:193-204.
9. Kohchi, C., and A. Toh-e. 1986. Cloning of Candida pelliculosa β-glucosidase gene and its expression in Saccharomyces cerevisiae. Mol. Gen. Genet. 203: 89-94.
10. Lamed, R., E. Setter, R. Kenig, and E. A. Bayer. 1983. The cellulosome - a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various catalytic activities. Biotechnol. Bioeng. Symp. 13:163-181.
11. Lipke, P. N., D. Wojciechowicz, and J. Kurjan. 1989. AGα1 is the structural gene for the Saccharomyces cerevisiae α-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol. Cell. Biol. 9:3155-3165.
12. Lipke, P. N., and J. Kurjan. 1992. Sexual agglutination in budding yeasts: structure, function, and regulation of adhesion glycoproteins. Microbiol. Rev. 56:180-194.
13. Lynd, L. R., J. H. Cushman, R. J. Nichols, and C. E. Wyman. 1991. Fuel ethanol from cellulosic biomass. Science 251:1318-1323.
14. Maiorella, B. L. 1985. Ethanol, p. 861-914. In M. Young (ed.), Comprehensive biotechnology, vol. 3. Pergamon Press, Oxford, United Kingdom.
15. Minamiguchi, K., T. Ooi, T. Kawaguchi, H. Okada, S. Murao, and M. Arai. 1995. Secretive expression of the Aspergillus aculeatus cellulase (FI-CMCase) by Saccharomyces cerevisiae. J. Ferment. Bioeng. 79:363-366.
16. Murai, T., M. Ueda, M. Yamamura, H. Atomi, Y. Shibasaki, N. Kamasawa, M. Osumi, T. Amachi, and A. Tanaka. 1997. Construction of a starch-utilizing yeast by cell surface engineering. Appl. Environ. Microbiol. 63: 1362-1366.
17. Murai, T., M. Ueda, H. Atomi, Y. Shibasaki, N. Kamasawa, M. Osumi, T. Kawaguchi, M. Arai, and A. Tanaka. 1997. Genetic immobilization of cellulase on the cell surface of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 48:499-503.
18. Murao, S., R. Sakamoto, and M. Arai. 1988. Cellulases of Aspergillus aculeatus. Methods Enzymol. 160:274-299.
19. Penttila, M. E., K. M. H. Nevalainen, A. Raynal, and J. K. C. Knowels. 1984. Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillus β-glucosidase. Mol. Gen. Genet. 194:494-499.
20. Raynal, A., and M. Guerineau. 1984. Cloning and expression of the structural gene for β-glucosidase of Kluyveromyces fragilis in Escherichia coli and Saccharomyces cerevisiae. Mol. Gen. Genet. 195:108-115.
21. Sakamoto, R., J. Kanamoto, M. Arai, and S. Murao. 1985. Purification and physicochemical properties of three β-glucosidases from Aspergillus aculeatus No. F-50. Agric. Biol. Chem. 49:1275-1281.
22. Sakamoto, R., M. Arai, and S. Murao. 1985. Enzymic properties of three β-glucosidases from Aspergillus aculeatus No. F-50. Agric. Biol. Chem. 49: 1283-1290.
23. Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
24. Tajima, M., Y. Nogi, and T. Fukasawa. 1985. Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast 1:67-77.
25. Tokatlidis, K., S. Salamitou, P. Beguin, P. Dhurjati, and J. P. Aubert. 1991. Interaction of the duplicated segment carried by Clostridium thermocellum. FEBS Lett. 291:185-188.
26. van Rensburg, P., W. H. van Zyl, and I. S. Pretorius. 1996. Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae. Curr. Genet. 30:246-250.
27. Weil, C. E., and P. Hanke. 1962. Thin-layer chromatography of maltooligosaccharides. Anal. Chem. 34:1736-1737.
28. Wojciechowicz, D., C.-F. Lu, J. Kurjan, and P. N. Lipke. 1993. Cell surface anchorage and ligand-binding domains of the Saccharomyces cerevisiae cell adhesion protein α-agglutinin, a member of the immunoglobulin superfamily. Mol. Cell. Biol. 13:2554-2563.
29. Wong, W. K. R., C. Curry, R. S. Parekh, S. R. Parekh, M. Wayman, R. W. Davies, D. G. Kilburn, and N. Skipper. 1988. Wood hydrolysis by Cellulomonas fimi endoglucanase and exoglucanase coexpressed as secreted enzymes in Saccharomyces cerevisiae. Bio/Technology 6:713-719.