Site-directed mutagenesis of active site contact residues in a hydrolytic abzyme: Evidence for an essential histidine involved in transition state stabilization

Journal of Molecular Biology

Volumn 267, Issue 5, 1997, Pages 1247-1257

  Miyashita, Hideaki ^a   Hara, Tomoko ^a   Tanimura, Ryuji ^a   Fukuyama, Shiro ^a   Cagnon, Christine ^a   Kohara, Atsuko ^a   Fujii, Ikuo ^a  

^a BIOMOL ENG RESEARCH INSTITUTE   (Japan)

Author keywords

3D molecular modeling; Catalytic antibody; Ester hydrolysis; Site directed mutagenesis; Transition state stabilization

Indexed keywords

CATALYTIC ANTIBODY; CHLORAMPHENICOL; CHLORAMPHENICOL DERIVATIVE; HAPTEN; HISTIDINE; IMMUNOGLOBULIN F(AB) FRAGMENT; PRODRUG;

AMINO ACID SEQUENCE; ANTIBODY COMBINING SITE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CATALYSIS; CHEMICAL MODIFICATION; CHEMICAL REACTION KINETICS; DRUG ACTIVATION; DRUG HYDROLYSIS; DRUG STABILITY; MOLECULAR INTERACTION; MOLECULAR MODEL; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THREE DIMENSIONAL IMAGING;

EID: 0031576985     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.0938     Document Type: Article

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