Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses

Applied Microbiology and Biotechnology

Volumn 55, Issue 4, 2001, Pages 471-475

  Shibasaki, S ^a   Ueda, M ^a   Iizuka, T ^b   Hirayama, M ^c   Ikeda, Y ^c   Kamasawa, N ^c   Osumi, M ^c   Tanaka, A ^a  

^a KYOTO UNIVERSITY   (Japan)

^b RATOC SYSTEM ENGINEERING Co Ltd   (Japan)

^c JAPAN WOMEN'S UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AGGLUTININ; ALPHA AGGLUTININ; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL SURFACE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FLUOROMETRY; GENETIC ENGINEERING; NONHUMAN; PH; PLASMID; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; SACCHAROMYCES CEREVISIAE;

ARABIDOPSIS; BASE SEQUENCE; CELL MEMBRANE; DNA PRIMERS; EVALUATION STUDIES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HYDROGEN-ION CONCENTRATION; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PROMOTER REGIONS (GENETICS); SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0035011537     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530000539     Document Type: Article

References (21)

1. Expression and analysis of the green fluorescent protein gene in the fission yeast Schizosaccharomyces pombe
2. In vivo dynamics of nuclear pore complexes in yeast
3. FACS-optimized mutants of the green fluorescent protein (GFP)
4. Improved green fluorescent protein by molecular evolution using DNA shuffling
5. In vivo examination of membrane protein localization and degradation with the green fluorescent protein
6. Studies on transformation of Escherichia coli with plasmids
7. Improved green fluorescence
8. Transformation of intact yeast cells treated with alkali cations
9. Relationship of actin organization to growth in the two forms of the dimorphic yeast Candida tropicalis
10. Construction of a starch-ultilizing yeast by cell surface engineering
11. Genetic immobilization of cellulase on the cell surface of Saccharomyces cerevisiae
12. Assimilation of cell oligosaccharides by a cell surface-engineered yeast expressing β-glucosidase and carboxymethylcellulase from Aspergillus aculeatus
13. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
14. Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae
15. Primary structure ot the Saccharomyces cerevisiae GAL7 gene
16. Molecular breeding of polysaccharide-utilizing yeast cells by cell surface engineering
17. Reversible denaturation of Aequorea green-fluorescent protein: Physical separation and characterization of renatured protein
18. Buffer effects on Eco RV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage
19. Optimized codon usage and chromophore mutations provide enhanced sensitivity with green fluorescent protein
20. Construction of engineered yeast with glucose-inducible emission of green fluorescence from cell surface
21. Avoiding interferences from Good's buffers: A contiguous series of non-complexing tertiary amine buffers convering the entire range of pH 3-11