Analysis of T-cell development by using short interfering RNA to knock down protein expression

Methods in Enzymology

Volumn 392, Issue , 2005, Pages 199-217

  Hernández Hoyos, Gabriela ^a   Alberola Ila, José ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; CELL PROTEIN; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; RETROVIRUS VECTOR; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ARTICLE; CELL CULTURE; CELL MATURATION; FETUS LIVER; FLOW CYTOMETRY; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENE TRANSFER; GENETIC ANALYSIS; LYMPHOID CELL LINE; NONHUMAN; ORGAN CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; STEM CELL; STROMA CELL; T LYMPHOCYTE; THYMOCYTE; THYMUS;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; GENETIC VECTORS; MICE; PROTEINS; RETROVIRIDAE; RNA INTERFERENCE; RNA, SMALL INTERFERING; T-LYMPHOCYTES;

MURINAE; UNIDENTIFIED RETROVIRUS;

EID: 11844258817     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(04)92012-5     Document Type: Article

References (32)

1. Agrawal N., Dasaradhi P.V., Mohmmed A., Malhotra P., Bhatnagar R.K., Mukherjee S.K. RNA interference: Biology, mechanism, and applications. Microbiol. Mol. Biol. Rev. 67:2003;657-685
2. Anderson M.K., Hernandez-Hoyos G., Dionne C.J., Arias A.M., Chen D., Rothenberg E.V. Definition of regulatory network elements for T cell development by perturbation analysis with PU.1 and GATA-3. Dev. Biol. 246:2002;103-121
3. Berns K., Hijmans E.M., Mullenders J., Brummelkamp T.R., Velds A., Heimerikx M., Kerkhoven R.M., Madiredjo M., Nijkamp W., Weigelt B., Agami R., Ge W., Cavet G., Linsley P.S., Beijersbergen R.L., Bernards R. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature. 428:2004;431-437
4. Bridge A.J., Pebernard S., Ducraux A., Nicoulaz A.L., Iggo R. Induction of an interferon response by RNAi vectors in mammalian cells. Nat. Genet. 34:2003;263-264
5. Brummelkamp T.R., Bernards R., Agami R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell. 2:2002a;243-247
6. Brummelkamp T.R., Bernards R., Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 296:2002b;550-553
7. Caplen N.J., Mousses S. Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells. Ann. N. Y. Acad. Sci. 1002:2003;56-62
8. Caplen N.J., Parrish S., Imani F., Fire A., Morgan R.A. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl. Acad. Sci. USA. 98:2001;9742-9747
9. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498
10. Elbashir S.M., Harborth J., Weber K., Tuschl T. Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods. 26:2002;199-213
11. Halene S., Wang L., Cooper R.M., Bockstoce D.C., Robbins P.B., Kohn D.B. Improved expression in hematopoietic and lymphoid cells in mice after transplantation of bone marrow transduced with a modified retroviral vector. Blood. 94:1999;3349-3357
12. Hannon G.J. RNA interference. Nature. 418:2002;244-251
13. Hemann M.T., Fridman J.S., Zilfou J.T., Hernando E., Paddison P.J., Cordon-Cardo C., Hannon G.J., Lowe S.W. An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo. Nat. Genet. 33:2003;396-400
14. Hernandez-Hoyos G., Anderson M.K., Wang C., Rothenberg E.V., Alberola-Ila J. GATA-3 expression is controlled by TCR signals and regulates CD4/CD8 differentiation. Immunity. 19:2003;83-94
15. Jenkinson E.J., Anderson G. Fetal thymic organ cultures. Curr. Opin. Immunol. 6:1994;293-297
16. Jenkinson E.J., Franchi L.L., Kingston R., Owen J.J. Effect of deoxyguanosine on lymphopoiesis in the developing thymus rudiment in vitro: Application in the production of chimeric thymus rudiments. Eur. J. Immunol. 12:1982;583-587
17. Khvorova A., Reynolds A., Jayasena S.D. Functional siRNAs and miRNAs exhibit strand bias. Cell. 115:2003;209-216
18. Lee N.S., Dohjima T., Bauer G., Li H., Li M.J., Ehsani A., Salvaterra P., Rossi J. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20:2002;500-505
19. McManus M.T., Sharp P.A. Gene silencing in mammals by small interfering RNAs. Nat. Rev. Genet. 3:2002;737-747
20. Michienzi A., Castanotto D., Lee N., Li S., Zaia J.A., Rossi J.J. RNA-mediated inhibition of HIV in a gene therapy setting. Ann. N. Y. Acad. Sci. 1002:2003;63-71
21. Naviaux R.K., Costanzi E., Haas M., Verma I.M. The pCL vector system: Rapid production of helper-free, high-titer, recombinant retroviruses. J. Virol. 70:1996;5701-5705
22. Paddison P.J., Caudy A.A., Hannon G.J. Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA. 99:2002;1443-1448
23. Paddison P.J., Silva J.M., Conklin D.S., Schlabach M., Li M., Aruleba S., Balija V., O'Shaughnessy A., Gnoj L., Scobie K., Chang K., Westbrook T., Cleary M., Sachidanandam R., McCombie W.R., Elledge S.J., Hannon G.J. A resource for large-scale RNA-interference-based screens in mammals. Nature. 428:2004;427-431
24. Pai S.Y., Truitt M.L., Ting C.N., Leiden J.M., Glimcher L.H., Ho I.C. Critical roles for transcription factor GATA-3 in thymocyte development. Immunity. 19:2003;863-875
25. Persengiev S.P., Zhu X., Green M.R. Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). RNA. 10:2004;12-18
26. Qin X.F., An D.S., Chen I.S., Baltimore D. Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc. Natl. Acad. Sci. USA. 100:2003;183-188
27. Robbins P.B., Skelton D.C., Yu X.J., Halene S., Leonard E.H., Kohn D.B. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells. Proc. Natl. Acad. Sci. USA. 95:1998;10182-10187
28. Rubinson D.A., Dillon C.P., Kwiatkowski A.V., Sievers C., Yang L., Kopinja J., Rooney D.L., Ihrig M.M., McManus M.T., Gertler F.B., Scott M.L., Van Parijs L. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33:2003;401-406
29. Scherr M., Battmer K., Dallmann I., Ganser A., Eder M. Inhibition of GM-CSF receptor function by stable RNA interference in a NOD/SCID mouse hematopoietic stem cell transplantation model. Oligonucleotides. 13:2003a;353-363
30. Scherr M., Battmer K., Ganser A., Eder M. Modulation of gene expression by lentiviral-mediated delivery of small interfering RNA. Cell Cycle. 2:2003b;251-257
31. Schwarz D.S., Hutvagner G., Du T., Xu Z., Aronin N., Zamore P.D. Asymmetry in the assembly of the RNAi enzyme complex. Cell. 115:2003;199-208
32. Sledz C.A., Holko M., de Veer M.J., Silverman R.H., Williams B.R. Activation of the interferon system by short-interfering RNAs. Nat. Cell Biol. 5:2003;834-839