An enzymatic assay for poly(ADP-ribose) polymerase-1 (PARP-1) via the chemical quantitation of NAD+: Application to the high-throughput screening of small molecules as potential inhibitors

Analytical Biochemistry

Volumn 326, Issue 1, 2004, Pages 78-86

  Putt, Karson S ^a   Hergenrother, Paul J ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DEATH; ENZYMES;

ANTI-CANCER THERAPIES; COMBINATORIAL LIBRARY; CONVENIENT ALTERNATIVES; FLUORESCENT COMPOUNDS; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT SCREENS; POLY(ADP-RIBOSE)-POLYMERASE 1 (PARP-1); SMALL MOLECULE INHIBITOR;

MOLECULES;

NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE 1; PROTEIN DERIVATIVE; UNCLASSIFIED DRUG;

ACCURACY; APOPTOSIS; ARTICLE; BIOTINYLATION; CATALYSIS; CATALYST; CELL DEATH; CELL FUNCTION; CELL SURVIVAL; COMPARATIVE STUDY; COMPLEX FORMATION; ENZYME ASSAY; IC 50; ISOTOPE LABELING; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; PRIORITY JOURNAL; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; SCREENING; TECHNIQUE;

EID: 0842324496     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.11.015     Document Type: Article

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