Both ATP sites of human P-glycoprotein are essential but not symmetric

Biochemistry

Volumn 38, Issue 42, 1999, Pages 13887-13899

  Hrycyna, Christine A ^a   Ramachandra, Muralidhara ^a,b   Germann, Ursula A ^a,c   Cheng, Priscilla Wu ^a,d   Pastan, Ira ^a   Gottesman, Michael M ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

^c VERTEX PHARMACEUTICALS   (United States)

^d JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; BINDING ENERGY; CELLS; CONFORMATIONS; DRUG INTERACTIONS; ENZYME INHIBITION; HYDROLYSIS; MUTAGENESIS;

CELL SURFACE; CONFORMATIONAL CHANGE; GLYCOPROTEIN; NUCLEOTIDE BINDING DOMAINS;

PROTEINS;

ADENOSINE TRIPHOSPHATE; GLYCOPROTEIN P; MUTANT PROTEIN; PRAZOSIN;

ARTICLE; BINDING SITE; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; DRUG TRANSPORT; HUMAN; HUMAN CELL; HYDROLYSIS; ISOTOPE LABELING; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN STRUCTURE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; ANTIBODIES, MONOCLONAL; ASPARAGINE; ASPARTIC ACID; AZIDES; BINDING SITES; BIOLOGICAL TRANSPORT; BODY TEMPERATURE; CELL MEMBRANE; CONSENSUS SEQUENCE; ENZYME ACTIVATION; FREEZING; HELA CELLS; HUMANS; P-GLYCOPROTEIN; PEPTIDE FRAGMENTS; PHOSPHORUS RADIOISOTOPES; PHOTOAFFINITY LABELS; POINT MUTATION; PRAZOSIN; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; VANADATES; VERAPAMIL;

EID: 0345583699     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi991115m     Document Type: Article

References (55)

1. Gottesman, M. M., Hrycyna, C. A., Schoenlein, P. V., Germann, U. A., and Pastan, I. (1995) Annu. Rev. Genet. 29, 607-649.
2. Gottesman, M. M., and Pastan, I. (1993) Annu. Rev. Biochem. 62, 385-427.
3. Chen, C.-j., Chin, J. E., Ueda, K., Clark, D. P., Pastan, I., Gottesman, M. M., and Roninson, I. B. (1986) Cell 47, 381-389.
4. Gottesman, M. M., and Pastan, I. (1988) J. Biol. Chem. 263, 12163-12166.
5. Hrycyna, C. A., Airan, L. E., Germann, U. A., Ambudkar, S. V., Pastan, I., and Gottesman, M. M. (1998) Biochemistry 37, 13660-13673.
6. Raviv, Y., Pollard, H. B., Bruggemann, E. P., Pastan, I., and Gottesman, M. M. (1990) J. Biol. Chem. 265, 3975-3980.
7. Higgins, C. F. (1992) Annu. Rev. Cell Biol. 8, 67-113.
8. Walker, J. E., Saraste, M., Runswick, M. J., and Gay, N. J. (1982) EMBO J. 1, 945-951.
9. Ames, G. F. (1986) Annu. Rev. Biochem. 55, 397-425.
10. Hyde, S. C., Emsley, P., Hartshorn, M. J., Mimmack, M. M., Gileadi, U., Pearce, S. R., Gallagher, M. P., Gill, D. R., Hubbard, R. E., and Higgins, C. F. (1990) Nature 346, 362-365.
11. Bakos, E., Klein, I., Welker, E., Szabo, K., Muller, M., Sarkadi, B., and Varadi, A. (1997) Biochem. J. 323, 777-783.
12. Hoof, T., Demmer, A., Hadam, M. R., Riordan, J. R., and Tummler, B. (1994) J. Biol. Chem. 269, 20575-20583.
13. Gottesman, M. M., Dey, S., Hrycyna, C. A., Ramachandra, M., Pastan, I., and Ambudkar, S. V. (1999) Annu. Rev. Pharmacol. Toxicicol. 39, 361-398.
14. Loo, T. W., and Clarke, D. M. (1995) J. Biol. Chem. 270, 21449-21452.
15. Azzaria, M., Schurr, E., and Gros, P. (1989) Mol. Cell. Biol. 9, 5289-5297.
16. Muller, M., Bakos, E., Welker, E., Varadi, A., Germann, U. A., Gottesman, M. M., Morse, B. S., Roninson, I. B., and Sarkadi, B. (1996) J. Biol. Chem. 271, 1877-1883.
17. Urbatsch, I. L., Sankaran, B., Bhagat, S., and Senior, A. E. (1995) J. Biol. Chem. 270, 26956-26961.
18. Senior, A. E., al-Shawi, M. K., and Urbatsch, I. L. (1995) FEBS Lett. 377, 285-289.
19. Senior, A. E., and Bhagat, S. (1998) Biochemistry 37, 831-836.
20. Hrycyna, C. A., Ramachandra, M., Ambudkar, S. V., Ko, Y. H., Pedersen, P. L., Pastan, I., and Gottesman, M. M. (1998) J. Biol. Chem. 273, 16631-16634.
21. Gadsby, D. C., and Nairn, A. C. (1999) Physiol. Rev. 79, S77-S107.
22. Wu, Y. C., and Horvitz, H. R. (1998) Cell 93, 951-960.
23. Ramachandra, M., Ambudkar, S. V., Gottesman, M. M., Pastan, I., and Hrycyna, C. A. (1996) Mol. Biol. Cell 7, 1485-1498.
24. Jones, D. H., and Howard, B. H. (1990) Biotechniques 8, 178-183.
25. Pastan, I., Gottesman, M. M., Ueda, K., Lovelace, E., Rutherford, A. V., and Willingham, M. C. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 4486-4490.
26. Elroy-Stein, O., and Moss, B. (1991) in Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kinston, R. E., Moore, D. D., Smith, J. A., Seidman, J. C., and Struhl, K., Eds.) pp 16.19.1-16.19.9, John Wiley and Sons, New York.
27. Earl, P. E., Cooper, N., and Moss, B. (1991) in Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kinston, R. E., Moore, D. D., Smith, J. A., Seidman, J. C., and Struhl, K., Eds.) pp 16.16.1-16.16.7, John Wiley and Sons, New York.
28. Ramachandra, M., Gottesman, M. M., and Pastan, I. (1998) Methods Enzymol. 292, 441-455.
29. Ramachandra, M., Ambudkar, S. V., Chen, D., Hrycyna, C. A., Dey, S., Gottesman, M. M., and Pastan, I. (1998) Biochemistry 37, 5010-5019.
30. Hrycyna, C. A., Ramachandra, M., Pastan, I., and Gottesman, M. M. (1998) Methods Enzymol. 292, 456-473.
31. Hamada, H., and Tsuruo, T. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7785-7789.
32. Georges, E., Bradley, G., Gariepy, J., and Ling, V. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 152-156.
33. Bruggemann, E. P., Chaudhary, V., Gottesman, M. M., and Pastan, I. (1991) Biotechniques 10, 202-209.
34. Weber, J., Hammond, S. T., Wilke-Mounts, S., and Senior, A. E. (1998) Biochemistry 37, 608-614.
35. Hung, L. W., Wang, I. X., Nikaido, K., Liu, P. Q., Ames, G. F., and Kim, S. H. (1998) Nature 396, 703-707.
36. Hrycyna, C. A., Zhang, S., Ramachandra, M., Ni, B., Pastan, I., and Gottesman, M. M. (1996) in Multidrug Resistance in Cancer Cells (Gupta, S., and Tsuruo, T., Eds.) pp 29-37, John Wiley & Sons, Ltd., New York.
37. Beck, W. T., Mueller, T. J., and Tanzer, L. R. (1979) Cancer Res. 39, 2070-2076.
38. Germann, U. A., Willingham, M. C., Pastan, I., and Gottesman, M. M. (1990) Biochemistry 29, 2295-2303.
39. Schinkel, A. H., Kemp, S., Dolle, M., Rudenko, G., and Wagenaar, E. (1993) J. Biol. Chem. 268, 7474-7481.
40. Urbatsch, I. L., Beaudet, L., Carrier, I., and Gros, P. (1998) Biochemistry 37, 4592-4602.
41. Bruggemann, E. P., Germann, U. A., Gottesman, M. M., and Pastan, I. (1989) J. Biol. Chem. 264, 15483-15488.
42. Dey, S., Ramachandra, M., Pastan, I., Gottesman, M. M., and Ambudkar, S. V. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 10594-10599.
43. Urbatsch, I. L., Sankaran, B., Weber, J., and Senior, A. E. (1995) J. Biol. Chem. 270, 19383-19390.
44. Cremo, C. R., Long, G. T., and Grammer, J. C. (1990) Biochemistry 29, 7982-7990.
45. Mechetner, E. B., Schott, B., Morse, B. S., Stein, W. D., Druley, T., Davis, K. A., Tsuruo, T., and Roninson, I. B. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 12908-12913.
46. Arceci, R. J., Stieglitz, K., and Bierer, B. E. (1992) Blood 80, 1528-1536.
47. Sharom, F. J. (1995) J. Bioenerg. Biomembr. 27, 15-22.
48. Ambudkar, S. V., Lelong, I. H., Zhang, J., Cardarelli, C. O., Gottesman, M. M., and Pastan, I. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8472-8476.
49. Georges, E., Zhang, J. T., and Ling, V. (1991) J. Cell. Physiol. 148, 479-484.
50. Al-Shawi, M. K., Urbatsch, I. L., and Senior, A. E. (1994) J. Biol. Chem. 269, 8986-8992.
51. Al-Shawi, M. K., and Senior, A. E. (1993) J. Biol. Chem. 268, 4197-4206.
52. Loo, T. W., and Clarke, D. M. (1995) J. Biol. Chem. 270, 22957-22961.
53. Senior, A. E., and Gadsby, D. C. (1997) Semin. Cancer Biol. 8, 143-150.
54. Shapiro, A. B., and Ling, V. (1998) Eur. J. Biochem. 254, 189-193.
55. Ambudkar, S. V., Cardarelli, C. O., Pashinsky, I., and Stein, W. D. (1997) J. Biol. Chem. 272, 21160-21166.