ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR

Seminars in Cancer Biology

Volumn 8, Issue 3, 1997, Pages 143-150

  Senior, Alan E ^a   Gadsby, David C ^b  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Catalytic cycles; CFTR; P glycoprotein

Indexed keywords

ABC TRANSPORTER; ADENOSINE TRIPHOSPHATE; ANION CHANNEL; GLYCOPROTEIN P; TRANSMEMBRANE CONDUCTANCE REGULATOR;

CATALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; DRUG HYDROLYSIS; DRUG TRANSPORT; GLYCOSYLATION; HYDROLYSIS; MULTIDRUG RESISTANCE;

EID: 0031156388     PISSN: 1044579X     EISSN: None     Source Type: Journal    
DOI: 10.1006/scbi.1997.0065     Document Type: Conference Paper

References (60)

1. 1. Gottesman MM, Pastan I (1993) Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu Rev Biochem 62:385-427
2. 2. Gros P. Buschman E (1993) The mouse multidrug resistance gene family: structural and functional analysis. Int Rev Cytol 137C:169-197
3. 3. Shapiro AB, Ling V (1995) Using purified P-glycoprotein to understand multidrug resistance. J Bioenerg Biomembr 27:7-13
4. 4. Stein WD (1997) Kinetics of the multidrug transporter (P-glycoprotein) and its reversal. Physiol Rev 77:545-590
5. 5. Ford JM, Hait WN (1990) Pharmacology of drugs that alter multidrug resistance in cancer. Pharmacol Rev 42:155-199
6. 6. Ford JM (1996) Experimental reversal of P-glycoprotein-mediated multidrug-resistance by pharmacological chemosensitisers. Eur J Cancer 32A:991-1001
7. 7. Roepe PD, Wei LY, Hoffman MM, Fritz F (1996) Altered drug translocation mediated by the MDR protein: direct, indirect, or both? J Bioenerg Biomembr 28:541-555
8. 8. Schindler M, Grabski S, Hoff E, Simon SM (1996) Defective pH regulation of acidic compartments in human breast cancer cells (MCF-7) is normalized in adriamycin-resistant cells (MCF-7adr). Biochemistry 35:2811-2817
9. 9. Loo TW, Clarke DM (1995) Membrane topology of a cysteineless mutant of human P-glycoprotein. J Biol Chem 270:843-848
10. 10. Kast C, Canfield V, Levenson R, Gros P (1996) Transmembrane organisation of mouse P-glycoprotein determined by epitope insertion and immunofluorescence. J Biol Chem 271:9240-9248
11. 11. Riordan JR, Rommens JM, Kerem B, Alon N, Rozmahel R, Grzelczak Z, Zielenski J, Lok S, Plavsic N, Chou JL, et al (1989) Identification of the cystic fibrosis gene: Cloning and characterization of the complementary DNA. Science 245:1066-1072
12. 12. Welsh MJ, Anderson MP, Rich DP, Berger HA, Denning GM, Ostedgaard LS, Sheppard DN, Cheng SH, Gregory RJ, Smith AE (1992) CFTR: a chloride channel with novel regulation. Neuron 8:821-829
13. 13. Riordan JR (1993) The cystic fibrosis transmembrane conductance regulator. Annu Rev Physiol 55:609-630
14. 14. Gadsby DC, Nagel G, Hwang TC (1995) The CFTR chloride channel of mammalian heart. Annu Rev Physiol 57:387-416
15. 15. Stutts MJ (1996) Regulation of other airway epithelial ion channels by CFTR (Dodge JA et al, eds), in Cystic Fibrosis -Current Topics Vol. 3. John Wiley, Chichester, U.K.
16. 16. Anderson MP, Berger HA, Rich DP, Gregory RJ, Smith AE, Welsh MJ (1991) Nucleoside triphosphates are required to open the CFTR chloride channel. Cell 67:775-784
17. - channel resembles the CFTR. Nature 360:81-84
18. 18. Schinkel AH, Kemp S, Dolle M, Rudenko G, Wagenaar E (1993) N-glycosylation and deletion mutants of the human MDR1 P-glycoprotein. J Biol Chem 268:7474-7481
19. 19. Germann UA (1996) P-glycoprotein - A mediator of multidrug resistance in tumour cells. Eur J Cancer 32A:927-944
20. 20. Marshall J, Fang S, Ostedgaard LS, O'Riordan CR, Ferrara D, Amara JF, Hoppe H, Scheule RK, Welsh MJ, Smith AE, Cheng SH (1994) Stoichiometry of recombinant CFTR in epithelial cells and its functional reconstitution into cells in vitro. J Biol Chem 269:2987-2995
21. - channel in CHO cells stably expressing the cystic fibrosis gene. Nature 352:628-631
22. - channel. Cell 66:1027-1036
23. 23. Picciotto M, Cohn J, Bertuzzi G, Greengard P, Nairn AC (1992) Phosphorylation of CFTR. J Biol Chem 267:12742-12752
24. - channel after mutagenesis of all PKA consensus phosphorylation sites. J Biol Chem 268:11304-11311
25. - channel by negative charge in the R-domain. J Biol Chem 268:20259-20267
26. 26. Jia YL, Mathews CJ, Hanrahan JW (1997) Phosphorylation by protein kinase C is required for acute activation of CFTR by protein kinase A. J Biol Chem 272:4978-4984
27. 27. Gadsby DC, Nairn AC (1994) Regulation of CFTR channel activity. Trends Biochem Sci 19:513-518
28. - channels by phosphorylation and ATP hydrolysis. Proc Natl Acad Sci USA 91:4698-4702
29. 29. Wilkinson DJ, Strong TV, Mansoura MK, Wood DL, Smith SS, Collins FS, Dawson DC (1997) CFTR activation: additive effects of stimulatory and inhibitory phosphorylation sites in the R domain. Am J Physiol (in press)
30. 30. Senior AE, Al-Shawi MK, Urbatsch IL (1995) The catalytic cycle of P-glycoprotein. FEBS Lett 377:285-289
31. 31. Liu R, Sharom FJ (1997) Fluorescence studies of the nucleotide binding domains of the P-glycoprotein multidrug transporter. Biochemistry 36:2836-2843
32. 32. Urbatsch IL, Sankaran B, Weber J, Senior AE (1995) P-glycoprotein is stably inhibited by vanadate-induced trapping of nucleotide at a single catalytic site. J Biol Chem 270:19383-19390
33. 33. Urbatsch IL, Sankaran B, Bhagat S, Senior AE (1995) Both P-glycoprotein nucleotide binding sites are catalytically active. J Biol Chem 270:26956-26961
34. 34. Sankaran B, Bhagat S, Senior AE (1997) Inhibition of P-glycoprotein ATPase activity by beryllium fluoride. Biochemistry 36:6847-6853
35. 35. Al-Shawi MK, Senior AE (1993) Characterisation of the adenosine triphosphatase activity of Chinese hamster P-glycoprotein. J Biol Chem 268:4197-4206
36. 36. Urbatsch IL, Al-Shawi MK, Senior AE (1994) Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein. Biochemistry 33:7069-7076
37. 37. Al-Shawi MK, Urbatsch IL, Senior AE (1994) Covalent inhibitors of P-glycoprotein ATPase activity. J Biol Chem 269:8986-8992
38. 38. Loo TW, Clarke DM (1995) Covalent modification of human P-glycoprotein mutants containing a single cysteine in either nucleotide binding fold abolishes drug-stimulated ATPase activity. J Biol Chem 270:22957-22961
39. 39. Loo TW, Clarke DM (1995) Rapid purification of human P-glycoprotein mutants expressed transiently in HEK293 cells by nickel-chelate chromatography and characterisation of their drug-stimulated ATPase activities. J Biol Chem 270:21449-21452
40. 40. Muller M, Bakos E, Welker E, Varadi A, Germann UA, Gottesman MM, Morse BS, Roninson IB, Sarkadi B (1996) Altered drug-stimulated ATPase activity in mutants of the human multidrug-resistance protein. J Biol Chem 271:1877-1883
41. 41. Azzaria M, Schurr E, Gros P (1989) Discrete mutations introduced in the predicted nucleotide binding sites of the mdr1 gene abolish its ability to confer multidrug resistance. Mol Cell Biol 9:5289-5297
42. 42. Scarborough GA (1995) Drug-stimulated ATPase activity of the human P-glycoprotein. J Bioenerg Biomemb 27:37-41
43. 43. Liu R, Sharom FJ (1996) Site-directed fluorescence labelling of P-glycoprotein on cysteine residues in the nucleotide binding domains. Biochemistry 35:11865-11873
44. 44. Urbatsch IL, Senior AE (1995) Effects of lipids on ATPase activity of purified Chinese hamster P-glycoprotein. Arch Biochem Biophys 316:135-140
45. 45. Rosenberg MF, Callaghan R, Ford RC, Higgins CF (1997) Structure of the multidrug-resistance P-glycoprotein to 2.5 nm resolution determined by electronmicroscopy and image analysis. J Biol Chem 272:10685-10694
46. 46. Li C, Ramjeesingh M, Wang W, Garami E, Hewryk M, Lee D, Rommens JM, Galley K, Bear C (1996) ATPase activity of CFTR. J Biol Chem 271:28463-28468
47. 47. Gunderson KL, Kopito RR (1995) Conformational states of CFTR associated with channel gating: the role of ATP binding and hydrolysis. Cell 82:231-239
48. - -channel opening and closing rates, confirming hydrolysis of two ATP molecules per gating cycle. Biophys J 70:127a
49. 49. Shultz BD, Bridges RJ, Frizzell RA (1996) Lack of conventional ATPase properties in CFTR chloride channel gating. J Membr Biol 151:63-75
50. 50. Gunderson KL, Kopito RR (1994) Effects of pyrophosphate and nucleotide analogs support a role for ATP hydrolysis in CFTR channel gating. J Biol Chem 269:19349-19353
51. 51. Carson MR, Travis SM, Welsh MJ (1995) The two nucleotidebinding domains of CFTR have distinct functions in controlling channel activity. J Biol Chem 270:1711-1717
52. 52. Manavalan, P, Dearborn DG, McPherson JM, Smith AE (1995) Sequence homologies between nucleotide binding regions of CFTR and G-proteins suggest structural and functional similarities. FEBS Lett 366:87-91
53. 53. Carson MR, Welsh MJ (1995) Structural and functional similarities between CFTR and GTP-binding proteins. Biophys J 69:2443-2448
54. - channel gating to an ATP hydrolysis cycle. Neuron 12:473-482
55. 55. Venglarik CJ, Schultz BD, Frizzell RA, Bridges RJ (1994) ATP alters current fluctuations of CFTR: evidence for a 3-state activation mechanism. J Gen Physiol 104:123-146
56. - channels: a kinetic analysis of channel regulation. Biophys J 66:1398-1403
57. 57. Senior AE, Al-Shawi MK, Urbatsch IL (1995) ATP hydrolysis by multidrug resistance protein from Chinese hamster ovary cells. J Bioenerg Biomemb 27:31-36
58. 58. Sharom FJ, Yu X, Chu HWK, Doige CA (1995) Characterisation of the ATPase activity of P-glycoprotein from multidrug resistant Chinese hamster ovary cells. Biochem J 308:381-390
59. 1-ATPase. Biochim Biophys Acta 1319:19-58
60. 60. Boguski MS, McCormick F (1993) Proteins regulating Ras and its relatives. Nature 366:643-654