Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms

Journal of Microbiological Methods

Volumn 41, Issue 2, 2000, Pages 85-112

  Moter, Annette ^a   Göbel, Ulf B ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

16S rRNA; FISH; Fluorescence; In situ hybridization; Probes

Indexed keywords

RNA 16S;

BACTERIUM; BLOOD CULTURE; ENVIRONMENT; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGUS; HUMAN; INTESTINE FLORA; METHODOLOGY; MICROORGANISM; MOUTH FLORA; NONHUMAN; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; REVIEW; TECHNIQUE; VETERINARY MEDICINE; WASTE WATER;

EID: 0343192453     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(00)00152-4     Document Type: Note

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119. Schönhuber W., Zarda B., Eix S., Rippka R., Herdmann M., Ludwig W., Amann R. In situ identification of Cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 65:1999;1259-1267.
120. Schramm A., De Beer D., Wagner M., Amann R. Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor. Appl. Environ. Microbiol. 64:1998;3480-3485.
121. Schramm A., De Beer D., van den Heuvel J.C., Ottengraf S., Amann A. Microscale distribution of populations and activities of Nitrosoira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors. Appl. Environ. Microbiol. 65:1999;3690-3696.
122. Schramm A., Santegoeds C.M., Nielsen H.K., Ploug H., Wagner M., Pribyl M., Wanner J., Amann R., de Beer D. On the occurence of anoxic microniches, denitrification, and sulfate reduction in aerated activated sludge. Appl. Environ. Microbiol. 65:1999;4189-4196.
123. Schuppler M., Wagner M., Schön G., Göbel U.B. In situ identification of nocardioform actinomycetes in activated sludge using fluorescent rRNA-targeted oligonucleotide probes. Microbiology. 144:1998;249-259.
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126. Snaidr J., Amann R., Huber I., Ludwig W., Schleifer K.-H. Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl. Environ. Microbiol. 63:1997;2884-2896.
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135. Steinert M., Emödi L., Amann R., Hacker J. Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii. Appl. Environ. Microbiol. 63:1997;2047-2053.
136. Sterflinger K., Hain M. In situ hybridization with rRNA targeted probes as a new tool for the detection of black yeasts and meristematic fungi. Stud. Mycol. 43:1999;23-30.
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145. Wagner M., Assmus B., Hartmann A., Hutzler P., Amann R. In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy. J. Microsc. 176:1994;181-187.
146. Wagner M., Schmid M., Juretschko S., Trebesius K.-H., Bubert A., Goebel W., Schleifer K.-H. In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes. FEMS Microbiol. Lett. 160:1998;159-168.
147. Wallner G., Amann R., Beisker W. Optimizing fluorescent in situ hybridization of suspended cells with rRNA-targeted oligonucleotide probes for the flow cytometric identification of microorganisms. Cytometry. 14:1993;136-143.
148. Wallner G., Steinmetz I., Bitter-Suermann D., Amann R. Combination of rRNA-targeted hybridization probes and immuno-probes for the identification of bacteria by flow cytometry. System Appl. Microbiol. 19:1996;569-576.
149. Wallner G., Fuchs B., Spring S., Beisker W., Amann R. Flow sorting of microorganisms for molecular analysis. Appl. Environ Microbiol. 63:1997;4223-4231.
150. Warren K.C., Coyne K.J., Waite J.H., Cary S.C. Use of methacrylate de-embedding protocols for in situ hybridization on semithin plastic sections with multiple detection strategies. J. Histochem. Cytochem. 46:1998;149-155.
151. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:1991;697-703.
152. Weiss P., Schweitzer B., Amann R., Simon M. Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow). Appl. Environ. Microbiol. 62:1996;1998-2005.
153. Wessendorf M.W., Brelje T.C. Which fluorophore is the brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas Red, and cyanine 3.18. Histochemistry. 98:1992;81-85.
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159. Zarda B., Amann R., Wallner W., Schleifer K.-H. Identification of single bacterial cells using digoxigenin-labelled, rRNA-targeted oligonucleotides. J. Gen. Microbiol. 137:1991;2823-2830.
160. Zimmer C., Wähnert U. Nonintercalating DNA-binding ligands: specificity of the interaction and their use as tools in biophysical, biochemical and biological investigations of the genetic materials. Prog. Biophys. Mol. Biol. 47:1986;31-112.