In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes

FEMS Microbiology Letters

Volumn 160, Issue 1, 1998, Pages 159-168

  Wagner, Michael ^a   Schmid, Michael ^a   Juretschko, Stefan ^a   Karl Heinz Trebesius, ^b   Bubert, Andreas ^c   Goebel, Werner ^c   Schleifer, Karl Heinz ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

16S rRNA; In situ hybridization; Listeria monocytogenes; mRNA; Virulence factor

Indexed keywords

ALCOHOL; DIGOXIGENIN; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN F(AB) FRAGMENT; LYSOZYME; MESSENGER RNA; PROTEINASE K; RIBOSOME RNA; RNA 16S; VIRULENCE FACTOR;

ARTICLE; BACTERIUM IDENTIFICATION; CELL HYBRIDIZATION; CONTROLLED STUDY; GENE AMPLIFICATION; LISTERIA MONOCYTOGENES; NONHUMAN; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL;

LISTERIA MONOCYTOGENES;

EID: 0032032304     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00570-3     Document Type: Article

References (28)

1. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K.-H. (1994) Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol. 60, 792-800.
2. Wagner, M., Rath, G., Amann, R., Koops, H.-P. and Schleifer, K.-H. (1995) In situ identification of ammonia-oxidizing bacteria. Syst. Appl. Microbiol. 17, 251-264.
3. Wagner, M., Amann, R., Kämpfer, P., Assmus, B., Hartman, A., Hutzler, P., Springer, N. and Schleifer, K.-H. (1994) Identification and in situ detection of gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17, 405-417.
4. Moter, A., Leist, G., Rudolph, R., Schrank, K., Choi, B.-K., Wagner, M. and Göbel, U.B. (1998) Fluorescence in-situ hybridization on in resin embedded tissue sections shows spatial distribution of yet uncultured treponemes (submitted).
5. Hahn, D., Amann, R.I. and Zeyer, J. (1993) Detection of mRNA in Streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes. Appl. Environ. Microbiol. 59, 2753-2757.
6. Hodson, R.E., Dustman, W.A., Garg, R.P. and Moran, M.A. (1995) In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol. 61, 4074-4082.
7. Hönerlage, W., Hahn, D. and Zeyer, J. (1995) Detection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization. Arch. Microbiol. 163, 235-241.
8. Hurek, T., Egener, T. and Reinhold-Hurek, B. (1997) Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the β subclass. J. Bacteriol. 179, 4172-4178.
9. Tolker-Nielsen, T., Holmstrøm, K. and Molin, S. (1997) Visualization of specific gene expression in single-cells of Salmonella typhimurium by in situ PCR. Appl. Environ. Microbiol. 63, 4195-4203.
10. Woese, C.R. (1987) Bacterial evolution. Microbiol. Rev. 51, 221-271.
11. Kuhn, M. and Goebel, W. (1995) Molecular studies of the virulence of Listeria monocytogenes. In: Genetic Engineering (Setlow, J.K., Ed.), Vol. 17, pp. 31-51. Plenum Press, New York.
12. Wuenscher, M.D., Köhler, S., Bubert, A., Gerike, U. and Goebel, W. (1993) The iap gene of Listeria monocytogenes is essential for cell viability and its gene product p60 has bacteriolytic activity. J. Bacteriol. 175, 3491-3501.
13. Bubert, A., Köhler, S. and Goebel, W. (1992) The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction. Appl. Environ. Microbiol. 58, 2625-2632.
14. Wang, R.F., Cao, W.-W. and Johnson, M.G. (1992) 16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods. Appl. Environ. Microbiol. 58, 2827-2831.
15. Manz, W., Amann, R., Ludwig, W., Wagner, M. and Schleifer, K.-H. (1992) Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15, 593-600.
16. Amann, R., Krumholz, L. and Stahl, D.A. (1990) Fluorescent oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology. J. Bacteriol. 172, 762-770.
17. Amann, R. (1995) In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Molecular Microbial Ecology Manual (Akkerman, A.D.C., van Elsas, J.D. and de Bruijn, F.J., Eds.), pp. 1-15. Kluwer Academic, Dordrecht.
18. Amann, R.I., Zarda, B., Stahl, D.A. and Schleifer, K.H. (1992) Identification of individual prokaryotic cells with enzyme-labeled, rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 58, 3007-3011.
19. Roller, C., Wagner, M., Amann, R., Ludwig, W. and Schleifer, K.-H. (1994) In situ probing of Gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides. Microbiology 140, 2849-2858.
20. Schönhuber, W., Fuchs, B., Juretschko, S. and Amann, R. (1997) Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl. Environ. Microbiol. 63, 3268-3273.
21. Lema, M.W., Brown, A. and Calkins, J.H. (1994) A general method for the extraction of DNA from bacteria. J. Microbiol. Methods 18, 167-172.
22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
23. Oelmüller, U., Krüger, N., Steinbüchel, A. and Friedrich, C.G. (1990) Isolation of prokaryotic RNA and detection of specific mRNA with biotinylated probes. J. Microbiol. Methods 11, 73-84.
24. Beimfohr, C., Krause, A., Amann, R., Ludwig, W. and Schleifer, K.-H. (1993) In situ identification of Lactococci, Enterococci and Streptococci. Syst. Appl. Microbiol. 16, 450-456.
25. De Los Reyes, F.L., Ritter, W. and Raskin, L. (1997) Group-specific small subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems. Appl. Environ. Microbiol. 63, 1107-1117.
26. Köhler, S., Leimeister-Wächter, M., Chakraborty, T., Lottspeich, F. and Goebel, W. (1990) The gene coding for the protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect. Immun. 58, 1943-1950.
27. Wesendorf, M.W. and Brelje, T.C. (1992) Which fluorophore is the brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas Red and cyanine 3.18. Histochemistry 98, 81-85.
28. Trebesius, K., Amann, R., Ludwig, W., Mühlegger, K. and Schleifer, K.-H. (1994) Identification of whole fixed bacterial cells with nonradioactive 23S rRNA-targeted polynucleotide probes. Appl. Environ. Microbiol. 60, 3228-3235.