An in situ hybridization protocol for detection and identification of planktonic bacteria

Systematic and Applied Microbiology

Volumn 19, Issue 3, 1996, Pages 403-406

  Glöckner, Frank Oliver ^a   Amann, Rudolf ^a   Alfreider, Albin ^b   Pernthaler, Jakob ^b   Psenner, Roland ^b   Trebesius, Karlheinz ^a   Schleifer, Karl Heinz ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

CY3; Epifluorescence microscopy; In situ hybridization; Oligonucleotide probe; Planktonic bacteria; vRNA

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; POLYCARBONATE;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; FILTER; FLUORESCENCE; IMAGING; IN SITU HYBRIDIZATION; LAKE; NONHUMAN; OLIGONUCLEOTIDE PROBE; PLANKTON; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS);

EID: 0029859085     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0723-2020(96)80069-5     Document Type: Article

References (20)

1. Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., Stahl, D. A.: Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56, 1919-1925 (1990a)
2. Amann, R. I., Krumholz, L., Stahl, D. A.: Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172, 762-770 (1990b)
3. Amann, R. I., Ludwig, W., Schleifer, K.-H.: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143-169 (1995)
4. DeLong, E. F., Wickham, G. S., Pace, N. R.: Phylogenetic stains: ribosomal RNA-based probes for the identification of single microbial cells. Science 243, 1360-1363 (1989)
5. Ferguson, R. L., Buckley, E. N., Palumbo, A. V.: Response of marine bacterioplankton to differential filtration and confinement. Appl. Environ. Microbiol. 47, 49-55 (1984)
6. Hicks, R., Amann, R. I., Stahl, D. A.: Dual staining of natural bacterioplankton with 4′,6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences. Appl. Environ. Microbiol. 58, 2158-2163 (1992)
7. Hobbie, J. E., Daley, R. J., Jasper, S.: Use of Nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33, 1225-1228 (1977)
8. Jones, J. G.: The effect of environmental factors on estimated viable and total populations of planktonic bacteria in lakes and experimental enclosures. Freshwater Biology 7, 67-91 (1977)
9. Kepner, R. L., Jr., Pratt, J. R.: Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol. Rev. 58, 603-615 (1994)
10. Kogure, K., Simidu, U., Taga, N.: A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25, 415-420 (1979)
11. Lee, S., Malone, C., Kemp, P. F.: Use of multiple 16S rRNA-targeted fluorescent probes to increase signal strength and measure cellular RNA from natural planktonic bacteria. Mar. Ecol. Prog. Ser. 101, 193-201 (1993)
12. Manz, W., Amann, R., Ludwig, W., Wagner, M., Schleifer, K.-H.: Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15, 593-600 (1992)
13. Murphy, J., Riley, J. P.: A modified solution method for the determination of phosphate in natural waters. Analyt. Chem. Acta 27, 31-36 (1962)
14. Schut, F., DeVries, E. J., Gottschal, J. C., Robertson, B. R., Harder, W., Prins, R. A., Button, D. K.: Isolation of typical marine bacteria by dilution culture: growth, maintenance, and characteristics of isolates under laboratory conditions. Appl. Environ. Microbiol. 59, 2150-2160, (1993)
15. Southwick, P. L., Ernst, L. A., Tauriello, E. W., Parker, S. R., Mujumdar, R. B., Mujumdar, S. R., Clever, H. A., Waggoner, A. S.: Cyanine dye labeling reagents - carboxymethylindocyanine succinimidyl esters. Cytometry 11, 418-430 (1990)
16. Staley, J. T., Konopka, A.: Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats. Ann. Rev. Microbiol. 39, 321-346 (1985)
17. Vollenweider, R. A.: Scientific fundamentals of eutrophication of lakes and flowing waters with special reference to phosphorus and nitrogen. OECD/DAS/CSI 68.27, 1-159 (1968)
18. Wagner, M., Amann, R., Lemmer, H., Schleifer, K. H.: Probing activated sludge with proteobacteria-specific oligonucleotides: inadequacy of culture-dependent methods for describing microbial community structure. Appl. Environ. Microbiol. 59, 1520-1525 (1993)
19. Wallner, G., Amann, R., Beisker, W.: Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14, 136-143 (1993)
20. Wessendorf, M. W., Brelje, T. C.: Which fluorophore is brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas Red, and cyanine 3.18. Histochemistry 98, 81-85 (1992)