Comparison of theoretical and experimental data to evaluate substrate diffusional limitations for crown ether- and methyl-β -cyclodextrin-activated serine protease subtilisin Carlsberg in tetrahydrofuran

Biotechnology and Bioengineering

Volumn 84, Issue 3, 2003, Pages 324-331

  Santos, Angélica M ^a   González, Maryliz ^a   Pacheco, Yamaris ^a   Griebenow, Kai ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

Crown ether; Cyclodextrin; Diffusional limitations; Non aqueous enzymology; Subtilisin Carlsberg

Indexed keywords

CATALYST ACTIVITY; CHEMICAL ACTIVATION; CROWN ETHERS; DIFFUSION; ORGANIC SOLVENTS; PARTICLE SIZE ANALYSIS; PROTEINS; SUBSTRATES;

DIFFUSIONAL LIMITATIONS;

BIOTECHNOLOGY;

CROWN ETHER; METHYL BETA CYCLODEXTRIN; N ACETYLPHENYLALANINE ETHYL ESTER; ORGANIC SOLVENT; PHENYLALANINE DERIVATIVE; PROPANOL; SUBTILISIN; TETRAHYDROFURAN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DIFFUSION; ENZYME ACTIVITY; ENZYME SUBSTRATE; ENZYMOLOGY; FREEZE DRYING; PARTICLE SIZE; TRANSESTERIFICATION;

BETA-CYCLODEXTRINS; COENZYMES; COMPUTER SIMULATION; CROWN ETHERS; CYCLODEXTRINS; ENZYME ACTIVATION; ENZYME STABILITY; FURANS; MICROSCOPY, ELECTRON, SCANNING; MODELS, CHEMICAL; PARTICLE SIZE; SERINE ENDOPEPTIDASES; SOLUTIONS; SOLVENTS; SUBSTRATE SPECIFICITY; SUBTILISINS;

EID: 0141756274     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10777     Document Type: Article

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