Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents?

Biotechnology and Bioengineering

Volumn 53, Issue 4, 1997, Pages 351-362

  Griebenow, Kai ^a   Klibanov, Alexander M ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

excipients; Fourier transform infrared (FTIR); lyophilization; organic solvents; subtilisin

Indexed keywords

CATALYST ACTIVITY; CONFORMATIONS; DEHYDRATION; ENZYME KINETICS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; MOLECULAR STRUCTURE; ORGANIC SOLVENTS; SOLUTIONS;

DIMETHYL SULFOXIDE (DMSO); ENZYME DENATURATION; LIGANDS; LYOPHILIZATION; SUBTILISIN;

ENZYMES;

SUBTILISIN;

ARTICLE; BIOENGINEERING; ENZYME ACTIVITY; ENZYME CONFORMATION; INFRARED SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE; QUANTITATIVE ASSAY;

EID: 0342275236     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970220)53:4<351::AID-BIT1>3.0.CO;2-M     Document Type: Article

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