Testing for diffusion limitations in salt-activated enzyme catalysts operating in organic solvents

Biotechnology and Bioengineering

Volumn 58, Issue 6, 1998, Pages 654-657

  Bedell, Bruce A ^a   Mozhaev, Vadim V ^a   Clark, Douglas S ^b   Dordick, Jonathan S ^a  

^a UNIVERSITY OF IOWA   (United States)

^b University of California   (United States)

Author keywords

Enzyme activation; Lyophilization with salt; Nonaqueous media; Substrate diffusion; Subtilisin Carlsberg; Thermolysin

Indexed keywords

CATALYST ACTIVITY; DIFFUSION; ORGANIC SOLVENTS; POTASSIUM COMPOUNDS; REACTION KINETICS; SALTS; SUBSTRATES;

ENZYMATIC ACTIVITY; LYOPHILIZATION;

ENZYMES;

ORGANIC SOLVENT; SODIUM CHLORIDE; THERMOLYSIN;

ARTICLE; CATALYST; DIFFUSION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; FREEZE DRYING; NONHUMAN; REACTION ANALYSIS;

BACILLUS; CATALYSIS; DIFFUSION; ENZYME ACTIVATION; KINETICS; SALTS; SOLVENTS; SUBSTRATE SPECIFICITY; SUBTILISINS; THERMOLYSIN;

EID: 0032551298     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980620)58:6<654::AID-BIT12>3.0.CO;2-7     Document Type: Article

References (25)

1. Adlercreutz, P. 1996. Modes of using enzymes in organic media, pp. 9-42. In: A. M. P. Koskinen and A. M. Klibanov (eds.), Enzymatic reactions in organic media. Blackie, London.
2. Bell, G., Hailing, P. J., Moore, B. D., Partridge, J., Rees, D. G. 1995. Biocatalyst behavior in low-water systems. Trends Biotechnol. 13: 469-473.
3. Blanch, H. W., Clark D. S. 1996. Biochemical engineering. Marcel Dek- ker, New York.
4. Clark, D. S. 1994. Can immobilization be exploited to modify enzyme activity? T. Biotechnol. 12: 439-443.
5. Dabulis, K., Klibanov, A. M. 1993. Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnol. Bioeng. 41: 566-574.
6. Economou, C., Chen, K., Arnold, F. H. 1992. Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E. Biotechnol. Bioeng. 39: 658-662.
7. Fersht, A. 1985. Enzyme structure and mechanism, 2nd edition. Freeman. London.
8. Kamat, S., Beckman, E. J., Russell, A. J. 1994. Role of diffusion in nonaqueous enzymology. Theory. Enzyme Microb. Technol. 14: 265-271.
9. Khmelnitsky, Y. L., Welch, S. H., Clark, D. S., Dordick, J. S. 1994. Salts dramatically enhance activity of enzymes suspended in organic solvents. J. Am. Chem. Soc. 116: 2647-2648.
10. Klibanov, A. M. 1997. Why are enzymes less active in organic solvents than in water? Trends Biotechnol. 15: 97-101.
11. Lin, G., Liu, H.-C. 1995. Ultrasound-promoted lipase-catalyzed reactions. Tetrahed. Lett. 36: 6067-6068.
12. Meyer, J. D., Matsuura, J. E., Kendrick, B. S., Evans, E. S., Evans, G. J., Manning, M. C. 1995. Solution behavior of α-chymotrypsin dissolved in nonpolar organic solvents via hydrophobic ion-pairing. Biopolymers 35: 451-456.
13. Paradkar, V. M., Dordick, J. S. 1994. Aqueous-like activity of αchymotrypsin dissolved in nearly anhydrous organic solvents. J. Am. Chem. Soc. 116: 5009-5010.
14. Parida, S., Dordick, J. S. 1993. Tailoring lipase specificity by solvent and substrate chemistries. J. Org. Chem. 58: 3238-3244.
15. Rich, J. O., Bedell, B. A., Dordick, J. S. 1995. Controling enzymecatalyzed regioselectivity in sugar ester synthesis. Biotechnol. Bioeng. 45: 426-434.
16. Rich, J. O., Dordick, J. S. 1997. Controlling subtilisin activity and selectivity in organic media by imprinting with nucleophilic substrates. J. Am. Chem. Soc. 119: 3245-3252.
17. Schmitke, J. L., Wescott, C. R., Klibanov, A. M. 1996. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents. J. Am. Chem. Soc. 118: 3360-3365.
18. Suzawa, V. M., Khmelnitsky, Yu. L., Giarto, L., Dordick, J. S., Clark, D. S. 1995. Suspended and immobilized chymotrypsin in organic media: Structure-function relationships revealed by ESR spectroscopy. J. Am. Chem. Soc. 117: 8435-8440.
19. Vulfson, E. N., Sarney, D. B., Law, B. A. 1991. Enhancement of subtilisincatalyzed interesterification in organic solvents by ultrasound irradiation. Enzyme Microb. Technol. 13: 123-126.
20. Wangikar, P. P., Graycar, T. P., Estell, D. A., Clark, D. S., Dordick, J. S. 1993. Protein and solvent engineering of subtilisin BPN' in nearly anhydrous organic media. J. Am. Chem. Soc. 115: 12231-12237.
21. Wangikar, P. P., Michels, P. C., Clark, D. S., Dordick, J. S. 1997. Structure and function of subtilisin BPN' solubilized in organic solvents. J. Am. Chem. Soc. 119: 70-76.
22. Wangikar, P. P., Rich, J. O., Clark, D. S., Dordick, J. S. 1994. Probing enzymic transition state nydrophobicities. Biochemistry 34: 12302-12310.
23. Wescott, C. R., Klibanov, A. M. 1993. Solvent variation inverts substrate specificity of an enzyme. J. Am. Chem. Soc. 115: 1629-1631.
24. Xu, Z.-F., Affleck, R., Wangikar, P., Suzawa, V., Dordick, J. S., Clark, D. S. 1994. Transition state stabilization of subtilisins in organic media. Biotechnol. Bioeng. 43: 515-520.
25. Yang, Z., Russell, A. J. 1996. Fundamentals of non-aqueous enzymology, pp. 43-69. In: A. M. P. Koskinen and A. M. Klibanov (eds.), Enzymatic reactions in organic media. Blackie, London.