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Volumn 42, Issue 28, 2003, Pages 3299-3301

A self-sufficient peroxide-driven hydroxylation biocatalyst

Author keywords

Biocatalysis; Cytochrome P450; Enzymes; Oxidation; Peroxygenase

Indexed keywords

HYDROXYLATION; SUBSTRATES;

EID: 0043269709     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200351434     Document Type: Article
Times cited : (201)

References (42)
  • 7
    • 0000738406 scopus 로고    scopus 로고
    • f) S. S. Stahl, J. A. Labinger, J. E. Bercaw, Angew. Chem. 1998, 110, 2298; Angew. Chem. Int. Ed. 1998, 37, 2181;
    • (1998) Angew. Chem. Int. Ed. , vol.37 , pp. 2181
  • 27
    • 0042488976 scopus 로고    scopus 로고
    • note
    • BMP consists of the first 463 amino acids. A 6-His sequence was included at the C-terminus with no notable effect on activity.
  • 29
    • 0042989737 scopus 로고    scopus 로고
    • note
    • -1), while similar TONs are reached. As a result of evolution in DMSO, 21B3 activity is not inhibited by DMSO until concentrations exceed 10%.
  • 30
    • 0041487089 scopus 로고    scopus 로고
    • note
    • Both HF87A and 21B3 are approximately 100% selective for the C-12 position of 12-pNCA, and result in nearly complete conversion of hydroxylated products into pNP.
  • 33
    • 0031823396 scopus 로고    scopus 로고
    • CPO is reported to oxidize unactivated C-H bonds in the radical probe substrate (trans-2-phenyl-l-methylcyclopropane) with a TON of 20, where only 31% of the product was alcohol; see P. H. Toy, M. Newcomb, L. P. Hager, Chem. Res. Toxicol. 1998, 11, 816.
    • (1998) Chem. Res. Toxicol. , vol.11 , pp. 816
    • Toy, P.H.1    Newcomb, M.2    Hager, L.P.3
  • 38
    • 0041988117 scopus 로고    scopus 로고
    • note
    • These P450s have unique active sites which include an Arg residue (see ref. [22]). Only fatty acids are accepted as substrates, and the carboxylate group of the substrate is positioned into the active site upon substrate binding. It is believed that the carboxylate group and the Arg residue work together to bind peroxide and facilitate O-O bond cleavage (see ref. [21]).
  • 42
    • 0041988116 scopus 로고    scopus 로고
    • note
    • Crude lysate or purified protein can be used.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.