Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases

Applied and Environmental Microbiology

Volumn 64, Issue 4, 1998, Pages 1175-1179

  Ratajczak, Andreas ^a   Geißdörfer, Walter ^a   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALKENE; BACTERIAL DNA; BACTERIAL ENZYME; MEMBRANE ENZYME; OXYGENASE;

ACINETOBACTER; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL METABOLISM; BIODEGRADATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OXIDATION; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE;

ACINETOBACTER; ACINETOBACTER SP.; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0345451054     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.4.1175-1179.1998     Document Type: Article

References (28)

1. Asperger, O., and H.-P. Kleber. 1991. Metabolism of alkanes by Acinetobacter, p. 323-351. In K. J. Towner, E. Bergogne-Berezin, and C. A. Fewson (ed.), The biology of Acinetobacter. Plenum Press, New York, N.Y.
2. Asperger, O., A. Naumann, and H.-P. Kleber. 1981. Occurrence of cytochrome P-450 in Acinetobacter strains after growth on n-hexadecane. FEMS Microbiol. Lett. 11:309-312.
3. Bustos, S., and R. Schleif. 1993. Functional domains of the AraC protein. Proc. Natl. Acad. Sci. USA 90:5638-5642.
4. de Groot, A., J.-J. Krijger, A. Filloux, and J. Tommassen. 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas putida strain WCS358. Mol. Gen. Genet. 250:491-501.
5. Eaton, R. W. 1997. p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate. J. Bacteriol. 179:3171-3180.
6. Gallegos, M.-T., C. Michan, and J. L. Ramos. 1993. The XylS/AraC family of regulators. Nucleic Acids Res. 21:807-810.
7. Geissdorfer, W., S. C. Frosch, G. Haspel, S. Ehrt, and W. Hillen. 1995. Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1. Microbiology 141:1425-1432.
8. Geissdorfer, W., A. Ratajczak, and W. Hillen. 1997. Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1. Gene 186:305-308.
9. Geissdorfer, W., A. Ratajczak, and W. Hillen. 1998. Transcription of ppK from Acinetobacter sp. strain ADP1 encoding a putative polyphosphate kinase is induced by phosphate starvation. Appl. Environ. Microbiol. 64:896-901.
10. Gralton, E. M., A. L. Campbell, and E. L. Neidle. 1997. Directed introduction of DNA cleavage sites to produce a high-resolution genetic and physical map of the Acinetobacter sp. strain ADP1 (BD413UE) chromosome. Microbiology 143:1345-1357.
11. Hardegger, M., A. K. Koch, U. A. Ochsner, A. Fiechter, and J. Reiser. 1994. Cloning and heterologous expression of a gene encoding an alkane-induced extracellular protein involved in alkane assimilation from Pseudomonas aeruginosa. Appl. Environ. Microbiol. 60:3679-3687.
12. Hunger, M., R. Schmucker, K. Veerebrahma, and W. Hillen. 1990. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87:45-51.
13. Juni, E., and A. Janik. 1969. Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J. Bacteriol. 98:281-288.
14. Kok, R. G., V. M. Christoffels, B. Vosman, and K. J. Hellingwerf. 1994. A gene of Acinetobacter calcoaceticus BD413 encodes a periplasmic peptidylprolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth. Biochim. Biophys. Acta 1219:601-606.
15. Kokotek, W., and W. Lotz. 1989. Construction of a lac-Z-kanamycin-resistance cassette, useful for site-directed mutagenesis and as a promotor probe. Gene 84:467-471.
16. Lee, N. R., M. O. Hwang, G. H. Jung, Y. S. Kim, and K. H. Min. 1996. Physical structure and expression of alkBA encoding alkane hydroxylase and rubredoxin reductase from Pseudomonas maltophilia. Biochem. Biophys. Res. Commun. 218:17-21.
17. Maeng, J. H., Y. Sakai, Y. Tani, and N. Kato. 1996. Isolation and characterization of a novel oxygenase that catalyzes the first step of n-alkane oxidation in Acinetobacter sp. strain M-1. J. Bacteriol. 178:3695-3700.
18. Palmen, R., B. Vosman, P. Buijsman, C. K. Breek, and K. J. Hellingwerf. 1993. Physiological characterization of natural transformation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 139:295-305.
19. Parche, S., W. Geissdörfer, and W. Hillen. 1997. Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1. J. Bacteriol. 179:4631-4634.
20. Rose, R. E. 1988. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16:355.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y.
22. Shanklin, J., C. Achim, H. Schmidt, B. G. Fox, and E. Munck. 1997. Mossbauer studies of alkane omega-hydroxylase: evidence for a diiron cluster in an integral-membrane enzyme. Proc. Natl. Acad. Sci. USA 94:2981-2986.
23. Shanklin, J., E. Whittle, and B. G. Fox. 1994. Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33:12787-12794.
24. Strätz, M., M. Mau, and K. N. Timmis. 1996. System to study horizontal gene exchange among microorganisms without cultivation of recipients. Mol. Microbiol. 22:207-215.
25. Suzuki, M., T. Hayakawa, J. P. Shaw, M. Rekik, and S. Harayama. 1991. Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system. J. Bacteriol. 173:1690-1695.
26. van Beilen, J. B., D. Penninga, and B. Witholt. 1992. Topology of the membrane-bound alkane hydroxylase of Pseudomonas oleovorans. J. Biol. Chem. 267:9194-9201.
27. van Beilen, J. B., M. G. Wubbolts, and B. Witholt. 1994. Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 5:161-174.
28. White, P. J., I. S. Hunter, and C. A. Fewson. 1991. Codon usage in Acinetobacter structural genes, p. 251-257. In K. J. Towner, E. Bergogne-Berezin, and C. A. Fewson (ed.), The biology of Acinetobacter. Plenum Press, New York, N.Y.