A functional assay for G-protein-coupled receptors using stably transformed insect tissue culture cell lines

Analytical Biochemistry

Volumn 320, Issue 1, 2003, Pages 88-103

  Knight, Peter J K ^a   Pfeifer, Tom A ^a   Grigliatti, Tom A ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Aequorin; Calcium flux; Cell based assay; Concentration activity curve; Functional assay; G protein coupled receptor; G 16; High 5 cell; Insect tissue culture; Luminescence; Protein expression; Sf9 cell

Indexed keywords

DRUG PRODUCTS; LUMINESCENCE; MAMMALS; PROTEINS; TISSUE; TISSUE CULTURE;

AEQUORIN; CALCIUM FLUX; CELL-BASED ASSAYS; FUNCTIONAL ASSAYS; G PROTEIN COUPLED RECEPTORS; PROTEIN EXPRESSIONS;

CELLS;

G PROTEIN COUPLED RECEPTOR; LIGAND; PHOSPHOLIPASE C; PHOTOPROTEIN;

ANIMAL CELL; ARTICLE; BETA CHAIN; BIOASSAY; CELL DIFFERENTIATION; CELL LINE; CELL TRANSFORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DIMERIZATION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; TISSUE CULTURE;

BOVINAE; INSECTA;

EID: 0042697145     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00354-3     Document Type: Article

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