Functional analysis by imaging of melanophore pigment dispersion of chimeric receptors constructed by recombinant polymerase chain reaction

Brain Research Protocols

Volumn 2, Issue 1, 1997, Pages 59-68

  McClintock, Timothy S ^a   Lerner, Michael R ^b  

^a UNIVERSITY OF KENTUCKY   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

G protein; G protein coupled receptor; Olfaction; Signal transduction

Indexed keywords

CATECHOLAMINE RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN;

ANIMAL CELL; ARTICLE; CHIMERA; MELANOPHORE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION; SMELLING;

ANIMALS; CELL CULTURE TECHNIQUES; CLONE CELLS; DNA PRIMERS; DNA, RECOMBINANT; GTP-BINDING PROTEINS; INDICATORS AND REAGENTS; MELANOPHORES; POLYMERASE CHAIN REACTION; RECEPTORS, ADRENERGIC, BETA-2; RECEPTORS, ODORANT; RECOMBINANT FUSION PROTEINS; TRANSFECTION; XENOPUS;

EID: 0031395288     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00030-5     Document Type: Article

References (20)

1. Anders R.A., Leof E.B. Chimeric granulocyte/macrophage colony-stimulating factor/transforming growth factor-β (TGF-β) receptors define a model system for investigating the role of homomeric and heteromeric receptors in TGF-β signalling. J. Biol. Chem. 271:1996;21758-21766.
2. Carrithers M.D., Lerner M.R. Synthesis and characterization of bivalent peptide ligands targeted to G-protein-coupled receptors. Chem. Biol. 3:1996;537-542.
3. Cusack B., Groshan K., McCormick D.J., Pang Y.-P., Perry R., Phung C.-T., Souder T., Richelson E. Chimeric rat/human neurotensin receptors localize a region of the receptor sensitive to binding of a novel, species-specific, picomolar affinity peptide. J. Biol. Chem. 271:1996;15054-15059.
4. Dascal N. The use of Xenopus oocytes for the study of ion channels. Crit. Rev. Biochem. 22:1987;317-387.
5. Graminski G.F., Jayawickreme C.K., Potenza M.N., Lerner M.R. Pigment dispersion in frog melanophores can be induced by a phorbol ester or stimulation of a recombinant receptor that activates phospholipase C. J. Biol. Chem. 268:1993;5957-5964.
6. Graminski G.F., Lerner M.R. A rapid bioassay for platelet-derived growth factor β-receptor tyrosine kinase function. Biotechnology. 12:1994;1008-1011.
7. R. Higuchi, Recombinant PCR, in: M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (Eds.), PCR Protocols: a Guide to Methods and Applications, Academic Press, London, 1990, pp. 177-183.
8. Jayawickreme C.K., Quillan J.M., Graminski G.F., Lerner M.R. Discovery and structure-function analysis of β-melanocyte-stimulating hormone antagonists. J. Biol. Chem. 269:1994;29846-29854.
9. m of transport activity. J. Biol. Chem. 267:1992;22550-22555.
10. Lerner M.R. Tools for investigating functional interactions between ligands and G-protein coupled receptors. Trends Neurosci. 17:1994;142-146.
11. M.R. Lerner, M.N. Potenza, G.F. Graminski, T.S. McClintock, C.K. Jayawickreme, S. Karne, A New Tool for Investigating G-Protein Coupled Receptors. CIBA Foundation Symposium: The Molecular Basis of Smell and Taste Transduction, Vol. 179, Wiley, Chichester, 1993, pp. 76-87.
12. McClintock T.S., Graminski G.F., Potenza M.N., Jayawickreme C.K., Roby-Shemkovitz A., Lerner M.R. Functional expression of recombinant G-protein coupled receptors monitored by video imaging of pigment movement in melanophores. Anal. Biochem. 209:1993;298-305.
13. McClintock T.S., Landers T.M., Gimelbrant A.A., Jayawickreme C.K., Fuller L.Z., Jackson B.A., Lerner M.R. Functional expression of olfactory-adrenergic receptor chimeras and intracellular retention of heterologously expressed olfactory receptors. Mol. Brain Res. 48:1997;270-278.
14. McClintock T.S., Rising J., Lerner M.R. Melanophore pigment dispersion responses to agonists show two patterns of sensitivity to inhibitors of cAMP-dependent protein kinase and protein kinase C. J. Cell. Physiol. 167:1996;1-7.
15. s protein-coupled receptors using a recombinant melanophore-based bioassay. Anal. Biochem. 206:1992;315-322.
16. 3 dopamine receptors. J. Neurosci. 14:1994;1463-1476.
17. Quillan J.M., Jayawickreme C.K., Lerner M.R. Combinatorial diffusion assay used to identify topically active melanocyte-stimulating hormone receptor antagonists. Proc. Natl. Acad. Sci. USA. 92:1995;2894-2898.
18. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Plainview, NY, 1989, pp. 1.38-1.46.
19. Sugden D., Rowe S.J. Protein kinase C activation antagonizes melatonin-induced pigment aggregation in Xenopus laevis melanophores. J. Cell Biol. 119:1992;1515-1521.
20. Wang W.W., Shahrestanifar M., Jin J., Howells R.D. Studies on mu and delta opioid receptor selectivity utilizing chimeric and site-mutagenized receptors. Proc. Natl. Acad. Sci. USA. 92:1995;12436-12440.