메뉴 건너뛰기




Volumn 9, Issue 6, 1998, Pages 624-631

Intracellular detection assays for high-throughput screening

Author keywords

[No Author keywords available]

Indexed keywords

CELL ENZYME; CELL RECEPTOR; ION CHANNEL;

EID: 0032402832     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(98)80141-9     Document Type: Review
Times cited : (67)

References (58)
  • 1
    • 0028198301 scopus 로고
    • Tools for investigating functional interactions between ligands and G-protein-coupled receptors
    • Lerner MR. Tools for investigating functional interactions between ligands and G-protein-coupled receptors. Trends Neurosci. 17:1994;142-146.
    • (1994) Trends Neurosci , vol.17 , pp. 142-146
    • Lerner, M.R.1
  • 2
    • 0031613239 scopus 로고
    • Functional screening of multiuse peptide libraries using melanophore bioassay
    • Jayawickreme CK, Jayawickreme SP, Lerner MR. Functional screening of multiuse peptide libraries using melanophore bioassay. Methods Mol Biol. 87:1988;119-128.
    • (1988) Methods Mol Biol , vol.87 , pp. 119-128
    • Jayawickreme, C.K.1    Jayawickreme, S.P.2    Lerner, M.R.3
  • 3
    • 0039410502 scopus 로고    scopus 로고
    • Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): High throughput assays in mammalian cells
    • Brauner-Osborne H, Brann R. Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): high throughput assays in mammalian cells. Eur J Pharmacol. 1:1996;93-102.
    • (1996) Eur J Pharmacol , vol.1 , pp. 93-102
    • Brauner-Osborne, H.1    Brann, R.2
  • 4
    • 0026784129 scopus 로고
    • Lux genes and the applications of bacterial bioluminescence
    • Stewart SAB, Williams P. lux genes and the applications of bacterial bioluminescence. J Gen Microbiol. 138:1992;1289-1300.
    • (1992) J Gen Microbiol , vol.138 , pp. 1289-1300
    • Stewart, S.A.B.1    Williams, P.2
  • 6
    • 0000949438 scopus 로고
    • Introduction to the bioluminescence of Medusae, with special reference to the photoprotein aequorin
    • Johnson FH, Shimomura O. Introduction to the bioluminescence of Medusae, with special reference to the photoprotein aequorin. Methods Enzymol. 57:1978;271-291.
    • (1978) Methods Enzymol , vol.57 , pp. 271-291
    • Johnson, F.H.1    Shimomura, O.2
  • 7
    • 0029740012 scopus 로고    scopus 로고
    • Chemiluminescence: Sensitive detection technology for reporter gene assays
    • Bronstein I, Martin CS, Fortin JJ, Olesen CE, Voyta JC. Chemiluminescence: sensitive detection technology for reporter gene assays. Clin Chem. 42:1996;1542-1546.
    • (1996) Clin Chem , vol.42 , pp. 1542-1546
    • Bronstein, I.1    Martin, C.S.2    Fortin, J.J.3    Olesen, C.E.4    Voyta, J.C.5
  • 8
    • 0032404444 scopus 로고    scopus 로고
    • Aequorea victoria bioluminescence moves into an exciting new era
    • Kendall JM, Badminton MN. Aequorea victoria bioluminescence moves into an exciting new era. Trends Biotechnol. 16:1998;216-224.
    • (1998) Trends Biotechnol , vol.16 , pp. 216-224
    • Kendall, J.M.1    Badminton, M.N.2
  • 10
    • 0029346997 scopus 로고
    • Involvement of MAP kinase in insulin signalling revealed by non-invasive imaging of luciferase gene expression in single living cells
    • Rutter GA, White MRH, Tavare JM. Involvement of MAP kinase in insulin signalling revealed by non-invasive imaging of luciferase gene expression in single living cells. Curr Biol. 5:1995;890-899.
    • (1995) Curr Biol , vol.5 , pp. 890-899
    • Rutter, G.A.1    White, M.R.H.2    Tavare, J.M.3
  • 11
    • 0030671161 scopus 로고    scopus 로고
    • Fluorescence correlation spectroscopy: Diagnosis for sparse molecules
    • Maiti S, Haupts U, Webb WW. Fluorescence correlation spectroscopy: diagnosis for sparse molecules. Proc Natl Acad Sci USA. 94:1997;11753-11757.
    • (1997) Proc Natl Acad Sci USA , vol.94 , pp. 11753-11757
    • Maiti, S.1    Haupts, U.2    Webb, W.W.3
  • 12
    • 0030087710 scopus 로고    scopus 로고
    • Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
    • Hiem R, Tsien RY. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol. 6:1996;178-182.
    • (1996) Curr Biol , vol.6 , pp. 178-182
    • Hiem, R.1    Tsien, R.Y.2
  • 13
    • 0029670330 scopus 로고    scopus 로고
    • Improved green fluorescence protein by molecular evolution using DNA shuffling
    • Crameri A, Whitehorn EA, Tale E, Stemmer WPC. Improved green fluorescence protein by molecular evolution using DNA shuffling. Nat Biotechnol. 14:1996;315-319.
    • (1996) Nat Biotechnol , vol.14 , pp. 315-319
    • Crameri, A.1    Whitehorn, E.A.2    Tale, E.3    Stemmer, W.P.C.4
  • 15
    • 0032031530 scopus 로고    scopus 로고
    • Fluorescent-protein biosensors: New tools for drug discovery
    • Giuliano K, Taylor DL. Fluorescent-protein biosensors: new tools for drug discovery. Trends Biotechnol. 16:1998;135-140.
    • (1998) Trends Biotechnol , vol.16 , pp. 135-140
    • Giuliano, K.1    Taylor, D.L.2
  • 16
    • 0024336003 scopus 로고
    • Fluorescent indicator for cytosolic calcium based on rhodamine and fluorescein chromophores
    • Minta A, Kao JP, Tsien RY. Fluorescent indicator for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem. 264:1989;8171-8178.
    • (1989) J Biol Chem , vol.264 , pp. 8171-8178
    • Minta, A.1    Kao, J.P.2    Tsien, R.Y.3
  • 17
    • 0028295796 scopus 로고
    • Resonance energy transfer: Methods and applications
    • Wu P, Brand L. Resonance energy transfer: methods and applications. Anal Biochem. 218:1994;1-13.
    • (1994) Anal Biochem , vol.218 , pp. 1-13
    • Wu, P.1    Brand, L.2
  • 19
    • 0031127384 scopus 로고    scopus 로고
    • Improved indicators of cell membrane potential that use fluorescence energy transfer
    • of special interest. This report describes improved probes for FRET-based detection of cellular membrane potential. The combination of fast and sensitive ratiometric signals makes this class of probes the reagents of choice for many biological applications, including certain cell-based ion channel assays.
    • Gonzalez JE, Tsien RY. Improved indicators of cell membrane potential that use fluorescence energy transfer. of special interest Chem Biol. 4:1997;269-277 This report describes improved probes for FRET-based detection of cellular membrane potential. The combination of fast and sensitive ratiometric signals makes this class of probes the reagents of choice for many biological applications, including certain cell-based ion channel assays.
    • (1997) Chem Biol , vol.4 , pp. 269-277
    • Gonzalez, J.E.1    Tsien, R.Y.2
  • 20
    • 0032472195 scopus 로고    scopus 로고
    • Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter
    • of outstanding interest. A powerful combinaton of β-lactamase as a reporter gene and a designed, cell-trapped, fluorogenic FRET substrate is described that is amenable to single live cell analysis. The enzyme amplification, excellent spectroscopic properties, and ease of cellular staining allow detection of as little as 50 molecules per cell and efficient clonal selection by FACS.
    • Zlokarnik G, Negulescu PA, Knapp TE, Mere L, Burres N, Feng L, Whitney M, Roemer K, Tsien RY. Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter. of outstanding interest Science. 279:1998;84-88 A powerful combinaton of β-lactamase as a reporter gene and a designed, cell-trapped, fluorogenic FRET substrate is described that is amenable to single live cell analysis. The enzyme amplification, excellent spectroscopic properties, and ease of cellular staining allow detection of as little as 50 molecules per cell and efficient clonal selection by FACS.
    • (1998) Science , vol.279 , pp. 84-88
    • Zlokarnik, G.1    Negulescu, P.A.2    Knapp, T.E.3    Mere, L.4    Burres, N.5    Feng, L.6    Whitney, M.7    Roemer, K.8    Tsien, R.Y.9
  • 21
    • 0030610646 scopus 로고    scopus 로고
    • 2+ based on green fluorescent proteins and calmodulin
    • 2+ indicators that operate via FRET between two GFPs. This paper demonstrates that FRET-based GFP probes can be targeted to specific organelles in live cells and monitor cellular signals including protein - protein interactions.
    • 2+ indicators that operate via FRET between two GFPs. This paper demonstrates that FRET-based GFP probes can be targeted to specific organelles in live cells and monitor cellular signals including protein - protein interactions.
    • (1997) Nature , vol.388 , pp. 882-887
    • Miyawaki, A.1    Llopis, J.2    Heim, R.3    McCaffery, J.M.4    Adams, J.A.5    Ikura, M.6    Tsien, R.Y.7
  • 22
    • 0029134581 scopus 로고
    • Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer
    • Mathis G. Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin Chem. 41:1995;1391-1397.
    • (1995) Clin Chem , vol.41 , pp. 1391-1397
    • Mathis, G.1
  • 24
    • 0031466995 scopus 로고
    • Direct visualization of the translocation of the γ-subspecies of protein kinase c in living cells using fusion proteins with green fluorescent protein
    • Sakai N, Sasaki K, Ikegaki N, Shirai Y, Ono Y, Saito N. Direct visualization of the translocation of the γ-subspecies of protein kinase c in living cells using fusion proteins with green fluorescent protein. J Cell Biol. 139:1993;1465-1476.
    • (1993) J Cell Biol , vol.139 , pp. 1465-1476
    • Sakai, N.1    Sasaki, K.2    Ikegaki, N.3    Shirai, Y.4    Ono, Y.5    Saito, N.6
  • 25
    • 0030736417 scopus 로고    scopus 로고
    • A β-arrestin/green fluorescent protein biosensor for detecting G-protein-coupled receptor activation
    • of special interest. An elegant demonstration of GFP localization to detect GPCR activation. The authors linked GFP to β-arrestin, a cytoplasmic protein which associates with a wide variety of GPCRs when they become phosphorylated following activation. This is a potentially generic assay for GPCR activation.
    • Barak LS, Ferguson SG, Zhang J, Carons MG. A β-arrestin/green fluorescent protein biosensor for detecting G-protein-coupled receptor activation. of special interest J Biol Chem. 272:1997;27497-27500 An elegant demonstration of GFP localization to detect GPCR activation. The authors linked GFP to β-arrestin, a cytoplasmic protein which associates with a wide variety of GPCRs when they become phosphorylated following activation. This is a potentially generic assay for GPCR activation.
    • (1997) J Biol Chem , vol.272 , pp. 27497-27500
    • Barak, L.S.1    Ferguson, S.G.2    Zhang, J.3    Carons, M.G.4
  • 26
    • 19644375284 scopus 로고    scopus 로고
    • FLIPR: A new instrument for accurate, high throughput optical screening
    • Shroeder KS, Neagle BD. FLIPR: a new instrument for accurate, high throughput optical screening. J Biomol Screening. 1:1996;75-80.
    • (1996) J Biomol Screening , vol.1 , pp. 75-80
    • Shroeder, K.S.1    Neagle, B.D.2
  • 27
    • 0032580202 scopus 로고    scopus 로고
    • Calcium oscillations increase the efficiency and specificity of gene expression
    • Dolmetsch RE, Xu K, Lewis RS. Calcium oscillations increase the efficiency and specificity of gene expression. Nature. 392:1998;933-936.
    • (1998) Nature , vol.392 , pp. 933-936
    • Dolmetsch, R.E.1    Xu, K.2    Lewis, R.S.3
  • 28
    • 0028897113 scopus 로고
    • Transcriptional regulation by extracellular signals: Mechanisms and specificity
    • Hill CS, Treisman R. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell. 80:1995;199-211.
    • (1995) Cell , vol.80 , pp. 199-211
    • Hill, C.S.1    Treisman, R.2
  • 30
    • 0027245830 scopus 로고
    • Substitution of three amino acids switches receptor specificity of Gqa to that of Gia
    • Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR. Substitution of three amino acids switches receptor specificity of Gqa to that of Gia. Nature. 363:1993;274-276.
    • (1993) Nature , vol.363 , pp. 274-276
    • Conklin, B.R.1    Farfel, Z.2    Lustig, K.D.3    Julius, D.4    Bourne, H.R.5
  • 31
    • 0028981057 scopus 로고
    • 16 couple a wide variety of receptors to phospholipase C
    • 16 couple a wide variety of receptors to phospholipase C. J Biol Chem. 270:1995;15175-15180.
    • (1995) J Biol Chem , vol.270 , pp. 15175-15180
    • Offermans, S.1    Simon, M.2
  • 32
    • 0031260472 scopus 로고    scopus 로고
    • A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor
    • 16 to generate a Ca-based assay for a variety of GPCRs in CHO cells. Larger signals found using mitochondrial-targeted apoaequorin compared with cytoplasmic form.
    • 16 to generate a Ca-based assay for a variety of GPCRs in CHO cells. Larger signals found using mitochondrial-targeted apoaequorin compared with cytoplasmic form.
    • (1997) Anal Biochem , vol.252 , pp. 115-126
    • Stables, J.1    Green, A.2    Marshall, F.3    Fraser, N.4    Knight, N.5    Sautel, M.6    Milligan, G.7    Lee, M.8    Rees, S.9
  • 33
    • 0031940393 scopus 로고    scopus 로고
    • Controlling signaling with specific designed Gi-coupled receptor
    • of special interest. A demonstration of the use of a chimeric Gqi G-protein to direct a Gi-type GPCR to a Ca signal. Receptor profiling carried out using HTS FLIPR technology and the Ca indicator Fluo-3.
    • Coward P, Wada HG, Falk MS, Chan SDH, Meng F, Akil H, Conklin BR. Controlling signaling with specific designed Gi-coupled receptor. of special interest Proc Natl Acad Sci USA. 95:1998;352-357 A demonstration of the use of a chimeric Gqi G-protein to direct a Gi-type GPCR to a Ca signal. Receptor profiling carried out using HTS FLIPR technology and the Ca indicator Fluo-3.
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 352-357
    • Coward, P.1    Wada, H.G.2    Falk, M.S.3    Chan, S.D.H.4    Meng, F.5    Akil, H.6    Conklin, B.R.7
  • 34
    • 0030176924 scopus 로고    scopus 로고
    • 16 as a universal G protein adapter: Implications for agonist screening strategies
    • 16 as a universal G protein adapter: implications for agonist screening strategies. Trends Pharmacol Sci. 17:1996;235-237.
    • (1996) Trends Pharmacol Sci , vol.17 , pp. 235-237
    • Milligan, G.1    Marshall, F.2    Rees, S.3
  • 35
    • 0031758718 scopus 로고    scopus 로고
    • Fluorescence techniques for measuring ion channel activity
    • of special interest. A lucid discussion of functional Ca-based screens for a variety of ligand and voltage-gated ion channels. Cell engineering is used to generate Ca signals from channels that did not flux Ca.
    • Velicelebi G, Stauderman KA, Varney MA, Akong M, Hess SD, Johnson ED. Fluorescence techniques for measuring ion channel activity. of special interest Methods Enzymol. 294:1998;20-47 A lucid discussion of functional Ca-based screens for a variety of ligand and voltage-gated ion channels. Cell engineering is used to generate Ca signals from channels that did not flux Ca.
    • (1998) Methods Enzymol , vol.294 , pp. 20-47
    • Velicelebi, G.1    Stauderman, K.A.2    Varney, M.A.3    Akong, M.4    Hess, S.D.5    Johnson, E.D.6
  • 37
    • 0028350832 scopus 로고
    • Characterization of the steady state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBac4(3)) in model systems and cells
    • Epps DE, Wolfe M, Groppi V. Characterization of the steady state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBac4(3)) in model systems and cells. Chem Phys Lipid. 69:1994;137.
    • (1994) Chem Phys Lipid , vol.69 , pp. 137
    • Epps, D.E.1    Wolfe, M.2    Groppi, V.3
  • 39
    • 0032087370 scopus 로고    scopus 로고
    • New assay technologies for high-throughput screening
    • Silverman L, Campbell, Broach JR. New assay technologies for high-throughput screening. Curr Opin Chem Biol. 2:1998;397-403.
    • (1998) Curr Opin Chem Biol , vol.2 , pp. 397-403
    • Silverman, L.1    Campbell2    Broach, J.R.3
  • 40
    • 0028850321 scopus 로고
    • Quantitative imaging of green fluorescent protein in cultured cells: Comparison of microscopic techniques, use in fusion proteins and detection limits
    • Niswender KD, Blackman SM, Rohde L, Magnuson MA, Piston DW. Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J Microsc. 180:1995;109-116.
    • (1995) J Microsc , vol.180 , pp. 109-116
    • Niswender, K.D.1    Blackman, S.M.2    Rohde, L.3    Magnuson, M.A.4    Piston, D.W.5
  • 41
    • 0029898330 scopus 로고    scopus 로고
    • Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of green fluorescent protein
    • Mitra RD, Silva CM, Youvan DC. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of green fluorescent protein. Gene. 173:1996;13-17.
    • (1996) Gene , vol.173 , pp. 13-17
    • Mitra, R.D.1    Silva, C.M.2    Youvan, D.C.3
  • 43
    • 0032503999 scopus 로고    scopus 로고
    • Specific covalent labeling of recombinant protein molecules inside live cells
    • of outstanding interest. The authors describe a membrane-permeant fluorescein derivative (FIAsH) with two trivalent arsenic atoms that selectively labels recombinant proteins containing a designed tetracysteine domain inside living cells. The approach offers several major advantages for biological applications, compared to GFP for example, such as compact size and potential to label domains within proteins. FIAsH could also serve as a FRET partner for GFP-tagged proteins.
    • Griffin BA, Adams SR, Tsien RY. Specific covalent labeling of recombinant protein molecules inside live cells. of outstanding interest Science. 281:1998;269-272 The authors describe a membrane-permeant fluorescein derivative (FIAsH) with two trivalent arsenic atoms that selectively labels recombinant proteins containing a designed tetracysteine domain inside living cells. The approach offers several major advantages for biological applications, compared to GFP for example, such as compact size and potential to label domains within proteins. FIAsH could also serve as a FRET partner for GFP-tagged proteins.
    • (1998) Science , vol.281 , pp. 269-272
    • Griffin, B.A.1    Adams, S.R.2    Tsien, R.Y.3
  • 44
    • 0031885535 scopus 로고    scopus 로고
    • Green fluorescent protein as a noninvasive intracelullar pH indicator
    • Kneen M, Farinas J, Li Y, Verkman AS. Green fluorescent protein as a noninvasive intracelullar pH indicator. Biophys J. 74:1998;1591.
    • (1998) Biophys J , vol.74 , pp. 1591
    • Kneen, M.1    Farinas, J.2    Li, Y.3    Verkman, A.S.4
  • 45
    • 0031865351 scopus 로고    scopus 로고
    • Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer
    • Mahajan NP, Linder K, Berry G, Gordon GW, Heim R, Herman B. Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nat Biotechnol. 16:1998;547-552.
    • (1998) Nat Biotechnol , vol.16 , pp. 547-552
    • Mahajan, N.P.1    Linder, K.2    Berry, G.3    Gordon, G.W.4    Heim, R.5    Herman, B.6
  • 46
    • 0030738524 scopus 로고
    • Monitoring protein - Protein interactions in intact eukaryotic cell by β-galactosidase complementation
    • Rossi F, Charlton CA, Blau HM. Monitoring protein - protein interactions in intact eukaryotic cell by β-galactosidase complementation. Proc Natl Acad Sci USA. 94:1995;8405-8410.
    • (1995) Proc Natl Acad Sci USA , vol.94 , pp. 8405-8410
    • Rossi, F.1    Charlton, C.A.2    Blau, H.M.3
  • 47
    • 0002231229 scopus 로고    scopus 로고
    • Fluorescence polarization assays for the detection of proteases and their inhibitors
    • Jolley M. Fluorescence polarization assays for the detection of proteases and their inhibitors. J Biomol Screening. 1:1996;33-38.
    • (1996) J Biomol Screening , vol.1 , pp. 33-38
    • Jolley, M.1
  • 48
    • 0029091561 scopus 로고
    • Fluorescence correlations, single molecule detection and large numbering. Applications in biotechnology
    • Rigler R. Fluorescence correlations, single molecule detection and large numbering. Applications in biotechnology. J Biotechnol. 41:1995;177-186.
    • (1995) J Biotechnol , vol.41 , pp. 177-186
    • Rigler, R.1
  • 49
    • 0031016380 scopus 로고    scopus 로고
    • Fluorescence correlation spectroscopy (FCS) - A highly sensitive method to analyze drug/target interactions
    • Sterrer S, Henco K. Fluorescence correlation spectroscopy (FCS) - a highly sensitive method to analyze drug/target interactions. J Recept Signal Transduct Res. 17:1997;511-520.
    • (1997) J Recept Signal Transduct Res , vol.17 , pp. 511-520
    • Sterrer, S.1    Henco, K.2
  • 50
    • 0032539688 scopus 로고    scopus 로고
    • Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
    • Kettling U, Koltermann A, Schwille P, Eigen M. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc Natl Acad Sci USA. 95:1998;1416-1420.
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 1416-1420
    • Kettling, U.1    Koltermann, A.2    Schwille, P.3    Eigen, M.4
  • 51
    • 0027227462 scopus 로고
    • Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
    • Mathis G. Rare earth cryptates and homogeneous fluoroimmunoassays with human sera. Clin Chem. 39:1993;1953-1959.
    • (1993) Clin Chem , vol.39 , pp. 1953-1959
    • Mathis, G.1
  • 52
    • 0028809366 scopus 로고
    • Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays
    • Dickson EF, Pollak A, Diamandis EP. Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays. J Photochem Photobiol B. 27:1995;3-19.
    • (1995) J Photochem Photobiol B , vol.27 , pp. 3-19
    • Dickson, E.F.1    Pollak, A.2    Diamandis, E.P.3
  • 53
    • 0031126530 scopus 로고    scopus 로고
    • Light on high-throughput screening: Fluorescence-based assay technologies
    • Rogers M. Light on high-throughput screening: fluorescence-based assay technologies. Drug Discov Today. 2:1997;156-160.
    • (1997) Drug Discov Today , vol.2 , pp. 156-160
    • Rogers, M.1
  • 54
    • 0030666868 scopus 로고    scopus 로고
    • The chemical-biological interface: Developments in automated and miniaturized screening technology
    • Houston JG, Banks M. The chemical-biological interface: developments in automated and miniaturized screening technology. Curr Opin Biotechnol. 8:1997;734-740.
    • (1997) Curr Opin Biotechnol , vol.8 , pp. 734-740
    • Houston, J.G.1    Banks, M.2
  • 55
    • 0032518347 scopus 로고    scopus 로고
    • A fluorescence polarization competition immunoassay for tyrosine kinases
    • Seethala R, Menzel R. A fluorescence polarization competition immunoassay for tyrosine kinases. Anal Biochem. 255:1998;257-262.
    • (1998) Anal Biochem , vol.255 , pp. 257-262
    • Seethala, R.1    Menzel, R.2
  • 56
    • 0002714048 scopus 로고    scopus 로고
    • Biological characterization of neurokinin antagonist discovery through screening of a combinatorial library
    • Appell KC, Chung TDY, Solly KJ, Chelsky D. Biological characterization of neurokinin antagonist discovery through screening of a combinatorial library. J Biomol Screening. 3:1998;19-27.
    • (1998) J Biomol Screening , vol.3 , pp. 19-27
    • Appell, K.C.1    Chung, T.D.Y.2    Solly, K.J.3    Chelsky, D.4
  • 57
    • 0031873720 scopus 로고    scopus 로고
    • Miniaturization technologies in HTS: How fast, how small, how soon?
    • Burbaum JJ. Miniaturization technologies in HTS: how fast, how small, how soon? Drug Discov Today. 3:1998;313-322.
    • (1998) Drug Discov Today , vol.3 , pp. 313-322
    • Burbaum, J.J.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.