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Volumn 9, Issue 5, 1998, Pages 523-527

Expression and recovery of functional G-protein-coupled receptors using baculovirus expression systems

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN;

EID: 0032192148     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0958-1669(98)80040-2     Document Type: Review
Times cited : (24)

References (48)
  • 3
    • 0023881356 scopus 로고
    • Trends in the development of baculovirus expression vectors
    • Luckow VA, Summers MD. Trends in the development of baculovirus expression vectors. Biotechnology. 6:1988;47-55.
    • (1988) Biotechnology , vol.6 , pp. 47-55
    • Luckow, V.A.1    Summers, M.D.2
  • 4
    • 0025193736 scopus 로고
    • The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector
    • Kuroda K, Geyer H, Geyer R, Doerfler W, Klenk H-D. The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology. 174:1990;418-429.
    • (1990) Virology , vol.174 , pp. 418-429
    • Kuroda, K.1    Geyer, H.2    Geyer, R.3    Doerfler, W.4    Klenk, H.-D.5
  • 5
    • 0028936425 scopus 로고
    • Expression of the extracellular domain of the thyrotropin receptor in the baculovirus system using a promoter active earlier than the polyhedrin promoter
    • Chazenbalk GD, Rapoport B. Expression of the extracellular domain of the thyrotropin receptor in the baculovirus system using a promoter active earlier than the polyhedrin promoter. J Biol Chem. 270:1995;1543-1549.
    • (1995) J Biol Chem , vol.270 , pp. 1543-1549
    • Chazenbalk, G.D.1    Rapoport, B.2
  • 6
    • 0024463925 scopus 로고
    • High-efficiency expression of a mammalian β-adrenergic receptors in baculovirus-infected insect cells
    • George ST, Arbabian MA, Ruoho AE, Kiely J, Malbon CC. High-efficiency expression of a mammalian β-adrenergic receptors in baculovirus-infected insect cells. Biochem Biophys Res Commun. 163:1989;1265-1269.
    • (1989) Biochem Biophys Res Commun , vol.163 , pp. 1265-1269
    • George, S.T.1    Arbabian, M.A.2    Ruoho, A.E.3    Kiely, J.4    Malbon, C.C.5
  • 8
    • 0026086012 scopus 로고
    • Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells
    • Parker EM, Kameyama K, Higashijima T, Ross EM. Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J Biol Chem. 266:1991;519-527.
    • (1991) J Biol Chem , vol.266 , pp. 519-527
    • Parker, E.M.1    Kameyama, K.2    Higashijima, T.3    Ross, E.M.4
  • 9
    • 0029045445 scopus 로고
    • Serotonergic antagonists differentially inhibit spontaneous activity and decrease ligand binding capacity of the rat 5HT2C receptor in Sf9 cells
    • Labrecque J, Fargin A, Bouvier M, Chidiac P, Dennis M. Serotonergic antagonists differentially inhibit spontaneous activity and decrease ligand binding capacity of the rat 5HT2C receptor in Sf9 cells. Mol Pharmacol. 48:1995;150-159.
    • (1995) Mol Pharmacol , vol.48 , pp. 150-159
    • Labrecque, J.1    Fargin, A.2    Bouvier, M.3    Chidiac, P.4    Dennis, M.5
  • 13
    • 0028876894 scopus 로고
    • Characterization of the rat m3 muscarinic acetylcholine receptor produced in insect cells infected with recombinant baculovirus
    • Vasudevan S, Hulme EC, Bach M, Haase W, Pavia J, Reilander H. Characterization of the rat m3 muscarinic acetylcholine receptor produced in insect cells infected with recombinant baculovirus. Eur J Biochem. 227:1995;466-475.
    • (1995) Eur J Biochem , vol.227 , pp. 466-475
    • Vasudevan, S.1    Hulme, E.C.2    Bach, M.3    Haase, W.4    Pavia, J.5    Reilander, H.6
  • 14
    • 0031569809 scopus 로고    scopus 로고
    • Palmitoylation of muscarinic acetylcholine receptor m2 subtypes: Reduction in their ability to activate G proteins by mutation of a putative palmitoylation site, cysteine 457, in the carboxyl-terminal tail
    • Hayashi MK, Haga T. Palmitoylation of muscarinic acetylcholine receptor m2 subtypes: reduction in their ability to activate G proteins by mutation of a putative palmitoylation site, cysteine 457, in the carboxyl-terminal tail. Arch Biochem Biophys. 340:1997;376-382.
    • (1997) Arch Biochem Biophys , vol.340 , pp. 376-382
    • Hayashi, M.K.1    Haga, T.2
  • 15
    • 0026540133 scopus 로고
    • Agonist-induced phosphorylation and desensitization of human m2 muscarinic cholinergic receptors in Sf9 insect cells
    • Richardson RM, Hosey MM. Agonist-induced phosphorylation and desensitization of human m2 muscarinic cholinergic receptors in Sf9 insect cells. J Biol Chem. 267:1992;22249-22255.
    • (1992) J Biol Chem , vol.267 , pp. 22249-22255
    • Richardson, R.M.1    Hosey, M.M.2
  • 17
    • 0028104820 scopus 로고
    • Phosphorylation and palmitoylation of the human D2L dopamine receptor in Sf9 cells
    • Ng GYK, O'Dowd BF, Caron M, Dennis M, Brann MR, George SR. Phosphorylation and palmitoylation of the human D2L dopamine receptor in Sf9 cells. J Neurochem. 63:1994;1589-1595.
    • (1994) J Neurochem , vol.63 , pp. 1589-1595
    • Ng, G.Y.K.1    O'Dowd, B.F.2    Caron, M.3    Dennis, M.4    Brann, M.R.5    George, S.R.6
  • 18
    • 0029919632 scopus 로고    scopus 로고
    • Expression of functional lutropin/choriogonadotropin receptor in the baculovirus system
    • Narayan P, Gray J, Puett D. Expression of functional lutropin/choriogonadotropin receptor in the baculovirus system. Mol Cell Endocrinol. 117:1996;95-100.
    • (1996) Mol Cell Endocrinol , vol.117 , pp. 95-100
    • Narayan, P.1    Gray, J.2    Puett, D.3
  • 19
    • 0032477746 scopus 로고    scopus 로고
    • Characterization of GRK2-catalyzed phosphorylation of the human substance P receptor in Sf9 membranes
    • Nishimura K, Warabi K, Roush ED, Frederick J, Schwinn DA, Kwatra MM. Characterization of GRK2-catalyzed phosphorylation of the human substance P receptor in Sf9 membranes. Biochemistry. 37:1998;1192-1198.
    • (1998) Biochemistry , vol.37 , pp. 1192-1198
    • Nishimura, K.1    Warabi, K.2    Roush, E.D.3    Frederick, J.4    Schwinn, D.A.5    Kwatra, M.M.6
  • 20
    • 0030806322 scopus 로고    scopus 로고
    • Two murine homologues of the human chemokine receptor CXCR4 mediating stromal cell-derived factor 1alpha activation of Gi2 are differentially expressed in vivo
    • Moepps B, Frodl R, Rodewald HR, Baggiolini M, Gierschik P. Two murine homologues of the human chemokine receptor CXCR4 mediating stromal cell-derived factor 1alpha activation of Gi2 are differentially expressed in vivo. Eur J Immunol. 27:1997;2102-2112.
    • (1997) Eur J Immunol , vol.27 , pp. 2102-2112
    • Moepps, B.1    Frodl, R.2    Rodewald, H.R.3    Baggiolini, M.4    Gierschik, P.5
  • 22
    • 0029787046 scopus 로고    scopus 로고
    • Agonist-induced modulation of inverse agonist efficacy at the β2-adrenergic receptor
    • Chidiac P, Nouet S, Bouvier M. Agonist-induced modulation of inverse agonist efficacy at the β2-adrenergic receptor. Mol Pharmacol. 50:1996;662-669.
    • (1996) Mol Pharmacol , vol.50 , pp. 662-669
    • Chidiac, P.1    Nouet, S.2    Bouvier, M.3
  • 23
    • 0028090035 scopus 로고
    • Reciprocal binding properties of 5-hydroxytryptamine type 2c receptor agonists and inverse agonists
    • Westphal R, Sanders-Bush E. Reciprocal binding properties of 5-hydroxytryptamine type 2c receptor agonists and inverse agonists. Mol Pharmacol. 46:1994;937-942.
    • (1994) Mol Pharmacol , vol.46 , pp. 937-942
    • Westphal, R.1    Sanders-Bush, E.2
  • 24
    • 0027959963 scopus 로고
    • High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype
    • Tiberi M, Caron MG. High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype. J Biol Chem. 269:1994;27925-27931.
    • (1994) J Biol Chem , vol.269 , pp. 27925-27931
    • Tiberi, M.1    Caron, M.G.2
  • 25
    • 0031029553 scopus 로고    scopus 로고
    • Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells
    • of special interest. This article illustrates that co-expression of GPCRs with distinct G-protein subunits in Sf9 cells can be used to assess the receptor - G-protein coupling selectivity.
    • Barr AJ, Brass LF, Manning DR. Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells. of special interest J Biol Chem. 272:1997;2223-2229 This article illustrates that co-expression of GPCRs with distinct G-protein subunits in Sf9 cells can be used to assess the receptor - G-protein coupling selectivity.
    • (1997) J Biol Chem , vol.272 , pp. 2223-2229
    • Barr, A.J.1    Brass, L.F.2    Manning, D.R.3
  • 26
    • 0030612004 scopus 로고    scopus 로고
    • Human 5-HT1 receptor subtypes exhibit distinct G protein coupling behaviors in membranes from Sf9 cells
    • of special interest. Reconstitution of G-protein subunits purified form Sf9 cells with membranes derived from Sf9 cells expressing specific GPCRs to assess the receptor - G-protein coupling selectivity. This article shows that using such in vitro reconstitution assay may offer a more quantitative assessment than using a whole cell co-expression system.
    • Clawges HM, Depree KM, Parker EM, Graber SG. Human 5-HT1 receptor subtypes exhibit distinct G protein coupling behaviors in membranes from Sf9 cells. of special interest Biochemistry. 36:1997;12930-12938 Reconstitution of G-protein subunits purified form Sf9 cells with membranes derived from Sf9 cells expressing specific GPCRs to assess the receptor - G-protein coupling selectivity. This article shows that using such in vitro reconstitution assay may offer a more quantitative assessment than using a whole cell co-expression system.
    • (1997) Biochemistry , vol.36 , pp. 12930-12938
    • Clawges, H.M.1    Depree, K.M.2    Parker, E.M.3    Graber, S.G.4
  • 28
    • 0028920262 scopus 로고
    • Palmitoylation of G-protein coupled receptors: A dynamic modification with functional consequences
    • Bouvier M, Moffett S, Loisel TP, Mouillac B, Hebert T, Chidiac P. Palmitoylation of G-protein coupled receptors: a dynamic modification with functional consequences. Biochem Soc Trends. 23:1995;116-120.
    • (1995) Biochem Soc Trends , vol.23 , pp. 116-120
    • Bouvier, M.1    Moffett, S.2    Loisel, T.P.3    Mouillac, B.4    Hebert, T.5    Chidiac, P.6
  • 29
    • 0029884416 scopus 로고    scopus 로고
    • Identification of the G protein-coupled receptor kinase phosphorylation sites in the human beta2-adrenergic receptor
    • Fredericks ZL, Pitcher JA, Lefkowitz RJ. Identification of the G protein-coupled receptor kinase phosphorylation sites in the human beta2-adrenergic receptor. J Biol Chem. 271:1996;13796-13803.
    • (1996) J Biol Chem , vol.271 , pp. 13796-13803
    • Fredericks, Z.L.1    Pitcher, J.A.2    Lefkowitz, R.J.3
  • 30
    • 0029811834 scopus 로고    scopus 로고
    • Palmitoylated cysteine 341 modulates phosphorylation of the β2-adrenergic receptor by cAMP-dependent protein kinase
    • Moffett S, Adam L, Bonin H, Loisel TP, Bouvier M, Mouillac B. Palmitoylated cysteine 341 modulates phosphorylation of the β2-adrenergic receptor by cAMP-dependent protein kinase. J Biol Chem. 271:1996;21490-21497.
    • (1996) J Biol Chem , vol.271 , pp. 21490-21497
    • Moffett, S.1    Adam, L.2    Bonin, H.3    Loisel, T.P.4    Bouvier, M.5    Mouillac, B.6
  • 31
    • 0028982276 scopus 로고
    • The beta-adrenergic receptor kinase (GRK2) is regulated by phospholipids
    • Onorato JJ, Gillis Me, Liu Y, Benovic JL, Ryoho AE. The beta-adrenergic receptor kinase (GRK2) is regulated by phospholipids. J Biol Chem. 270:1995;21346-21353.
    • (1995) J Biol Chem , vol.270 , pp. 21346-21353
    • Onorato, J.J.1    Gillis, Me.2    Liu, Y.3    Benovic, J.L.4    Ryoho, A.E.5
  • 34
    • 0029753014 scopus 로고    scopus 로고
    • Agonist stimulation increases the turnover rate of β2AR-bound palmitate and promotes receptor depalmitoylation
    • Loisel TP, Adam L, Hebert T, Bouvier M. Agonist stimulation increases the turnover rate of β2AR-bound palmitate and promotes receptor depalmitoylation. Biochemistry. 35:1996;15923-15932.
    • (1996) Biochemistry , vol.35 , pp. 15923-15932
    • Loisel, T.P.1    Adam, L.2    Hebert, T.3    Bouvier, M.4
  • 36
    • 0031569809 scopus 로고    scopus 로고
    • Palmitoylation of muscarinic acetylcholine receptor m2 subtypes: Reduction in their ability to activate G proteins by mutation of a putative palmitoylation site, cysteine 457, in the carboxyl-terminal tail
    • Hayashi MK, Haga T. Palmitoylation of muscarinic acetylcholine receptor m2 subtypes: reduction in their ability to activate G proteins by mutation of a putative palmitoylation site, cysteine 457, in the carboxyl-terminal tail. Arch Biochem Biophys. 340:1997;376-382.
    • (1997) Arch Biochem Biophys , vol.340 , pp. 376-382
    • Hayashi, M.K.1    Haga, T.2
  • 39
    • 0025728196 scopus 로고
    • Purification and functional characterization of the human beta2-adrenergic receptor produced in baculovirus-infected insect cells
    • Reilander H, Boege F, Vasudevan S, Maul G, Hekman M, Dees C, Hampe W, Helmreich EJM, Michel H. Purification and functional characterization of the human beta2-adrenergic receptor produced in baculovirus-infected insect cells. FEBS. 282:1991;441-444.
    • (1991) FEBS , vol.282 , pp. 441-444
    • Reilander, H.1    Boege, F.2    Vasudevan, S.3    Maul, G.4    Hekman, M.5    Dees, C.6    Hampe, W.7    Helmreich, E.J.M.8    Michel, H.9
  • 40
    • 0028809411 scopus 로고
    • Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor
    • Kobilka BK. Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor. Anal Biochem. 231:1995;269-271.
    • (1995) Anal Biochem , vol.231 , pp. 269-271
    • Kobilka, B.K.1
  • 42
    • 0029924772 scopus 로고    scopus 로고
    • Photoaffinity labeling of the human brain cholecystokinin receptor overexpressed in insect cells
    • Gimpl G, Anders J, Thiele C, Fahrenholtz F. Photoaffinity labeling of the human brain cholecystokinin receptor overexpressed in insect cells. Eur J Biochem. 237:1996;768-777.
    • (1996) Eur J Biochem , vol.237 , pp. 768-777
    • Gimpl, G.1    Anders, J.2    Thiele, C.3    Fahrenholtz, F.4
  • 43
    • 0026564303 scopus 로고
    • Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide
    • Guan X-M, Kobilka TS, Kobilka BK. Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide. J Biol Chem. 267:1992;21995-21998.
    • (1992) J Biol Chem , vol.267 , pp. 21995-21998
    • Guan, X.-M.1    Kobilka, T.S.2    Kobilka, B.K.3
  • 44
    • 0030859541 scopus 로고    scopus 로고
    • Agonist induced conformational changes in transmembrane domains III and VI of the β2-adrenergic receptor
    • of outstanding interest. Illustration of the use of GPCR purified to homogeneity from Sf9 cells for biophysical studies aimed at directly assessing the conformational changes occuring upon receptor activation.
    • Gether U, Lin S, Ghanouni P, Ballesteros JA, Weinstein H, Kobilka BK. Agonist induced conformational changes in transmembrane domains III and VI of the β2-adrenergic receptor. of outstanding interest EMBO J. 16:1997;6737-6747 Illustration of the use of GPCR purified to homogeneity from Sf9 cells for biophysical studies aimed at directly assessing the conformational changes occuring upon receptor activation.
    • (1997) EMBO J , vol.16 , pp. 6737-6747
    • Gether, U.1    Lin, S.2    Ghanouni, P.3    Ballesteros, J.A.4    Weinstein, H.5    Kobilka, B.K.6
  • 46
    • 0030700766 scopus 로고    scopus 로고
    • Recovery of homogeneous and functional beta-2 adrenergic receptors form extracellular baculovirus particles
    • of outstanding interest. The first report documenting that fully processed and functional GPCR are expressed at the surface of extracellular baculovirus particles released from baculovirus infected cells. This may offer an advantageous source of receptor for biophysical and structural studies.
    • Loisel TP, Ansanay H, St-Onge S, Gay B, Boulanger P, Strosberg AD, Marullo S, Bouvier M. Recovery of homogeneous and functional beta-2 adrenergic receptors form extracellular baculovirus particles. of outstanding interest Nat Biotechnol. 15:1997;1300-1304 The first report documenting that fully processed and functional GPCR are expressed at the surface of extracellular baculovirus particles released from baculovirus infected cells. This may offer an advantageous source of receptor for biophysical and structural studies.
    • (1997) Nat Biotechnol , vol.15 , pp. 1300-1304
    • Loisel, T.P.1    Ansanay, H.2    St-Onge, S.3    Gay, B.4    Boulanger, P.5    Strosberg, A.D.6    Marullo, S.7    Bouvier, M.8
  • 47
    • 0026646267 scopus 로고
    • Expression of feline leukaemia virus gp85 and Gag proteins and assembly into virus-like particles using the baculovirus expression system
    • Thomsen DR, Meyer AL, Post LE. Expression of feline leukaemia virus gp85 and Gag proteins and assembly into virus-like particles using the baculovirus expression system. J Gen Virol. 73:1992;1819-1824.
    • (1992) J Gen Virol , vol.73 , pp. 1819-1824
    • Thomsen, D.R.1    Meyer, A.L.2    Post, L.E.3
  • 48
    • 0029068263 scopus 로고
    • Incorporation of pseudorabies virus gD into human immunodeficiency virus type 1 Gag particles produced in baculovirus-infected cells
    • Garnier L, Ravallec M, Blanchard P, Chaabihi H, Bossy J-P, Dechauvelle G, Jestin A, Cerutti M. Incorporation of pseudorabies virus gD into human immunodeficiency virus type 1 Gag particles produced in baculovirus-infected cells. J Virol. 69:1995;4060-4068.
    • (1995) J Virol , vol.69 , pp. 4060-4068
    • Garnier, L.1    Ravallec, M.2    Blanchard, P.3    Chaabihi, H.4    Bossy, J.-P.5    Dechauvelle, G.6    Jestin, A.7    Cerutti, M.8


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