High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression

Journal of Molecular Biology

Volumn 318, Issue 2, 2002, Pages 395-405

  Rungpragayphan, Suang ^a   Kawarasaki, Yasuaki ^a   Imaeda, Takao ^b   Kohda, Katsunori ^b   Nakano, Hideo ^a   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b Rd Management Section   (Japan)

Author keywords

In vitro coupled transcription translation; PCR library; Protein library; Single chain antibody; Single molecule PCR

Indexed keywords

ALBUMIN ANTIBODY; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY SCREENING; ARTICLE; GENE AMPLIFICATION; GENE CONSTRUCT; GENE EXPRESSION; GENETIC TRANSCRIPTION; IN VITRO STUDY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA TRANSLATION;

EID: 0036306953     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00094-3     Document Type: Article

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