Lack of sugar discrimination by human Pol μ requires a single glycine residue

Nucleic Acids Research

Volumn 31, Issue 15, 2003, Pages 4441-4449

  Ruiz, José F ^a   Juárez, Raquel ^a   García Díaz, Miguel ^a   Terrados, Gloria ^a   Picher, Angel J ^a   González Barrera, Sergio ^a   Fernández de Henestrosá, Antonio R ^a   Blanco, Luis ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE; DNA DIRECTED DNA POLYMERASE; DNA POLYMERASE; GLYCINE; NUCLEIC ACID; PRIMER DNA; PRIMER RNA; RIBONUCLEOTIDE; SUGAR; CARBOHYDRATE; DNA; DNA POLYMERASE MU; DNA POLYMERASE X; RNA; RNA PRIMERS;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA REPAIR; DNA STRAND BREAKAGE; HUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING; AMINO ACID SEQUENCE; BASE PAIRING; BIOSYNTHESIS; CHEMISTRY; DNA TEMPLATE; ENZYME SPECIFICITY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE PAIRING; BASE SEQUENCE; CARBOHYDRATES; DEOXYRIBONUCLEOTIDES; DNA; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; GLYCINE; HUMANS; MOLECULAR SEQUENCE DATA; RIBONUCLEOTIDES; RNA; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC;

EID: 0042662911     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg637     Document Type: Article

References (32)

1. Joyce, C.M. (1997) Choosing the right sugar: How polymerases select a nucleotide substrate. Proc. Natl Acad. Sci. USA, 94, 1619-1622.
2. Astatke, M., Ng, K., Grindley, N.D.F. and Joyce, C.M. (1998) A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides. Proc. Natl Acad. Sci. USA, 95, 3402-3407.
3. Cases-González, C.E., Gutiérrez-Rivas, M. and Menéndez-Arias, L. (2000) Coupling ribose selection to fidelity of DNA synthesis. The role of Tyr-115 of human immunodeficiency virus type 1 reverse transcriptase. J. Biol. Chem., 275, 19759-19767.
4. Gao, G., Orlova, M., Georgiadis, M.M., Hendrickson, W.A. and Goff, S.P. (1997) Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection. Proc. Natl Acad. Sci. USA, 94, 407-411.
5. Bonnin, A, Lázaro, J.M., Blanco, L. and Salas, M. (1999) A single tyrosine prevents insertion of ribonucleotides in the eukaryotic-type phi29 DNA polymerase. J. Mol. Biol., 290, 241-251.
6. Patel, P.H. and Loeb, L.A. (2000) Multiple amino acid substitutions allow DNA polymerases to synthesize RNA. J. Biol. Chem., 275, 40266-40272.
7. Ogawa, M., Tosaka, A., Ito, Y., Yoshida, S. and Suzuki, M. (2001) Enhanced ribonucleotide incorporation by an O-helix mutant of Thermus aquaticus DNA polymerase I. Mutat. Res., 485, 197-207.
8. Bassing, C.H., Swat, W. and Alt, F.W. (2002) The mechanism and regulation of chromosomal V(D)J recombination. Cell, 109, S45-S55.
9. Kato, K.I., Goncalves, J.M., Houts, G.E. and Bollum, F.J. (1967) Deoxynucleotide-polymerizing enzymes of calf thymus gland. II. Properties of the terminal deoxynucleotidyltransferase. J. Biol. Chem., 242, 2780-2789.
10. Boulé, J.B., Rougeon, F. and Papanicolaou, C. (2001) Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. J. Biol. Chem., 276, 31388-31393.
11. Holm, L. and Sander, C. (1995) DNA polymerase β belongs to an ancient nucleotidyltransferase superfamily. Trends Biochem. Sci., 20, 345-347.
12. Aravind, L. and Koonin, E.V. (1999) DNA polymerase β-like nucleotidyltransferase superfamily: identification of three new families, classification and evolutionary history. Nucleic Acids Res., 27, 1609-1618.
13. SenGupta, D.N., Zmudzka, B.Z., Kumar, P., Cobianchi, F., Skowronski, J. and Wilson, S.H. (1986) Biochem. Biophys. Res. Commun. 136, 341-347.
14. García-Díaz, M., Domínguez, O., López-Fernández, L.A., Laín de Lera, T., Saníger, M.L., Ruiz, J.F., Párraga, M., García-Ortiz, M.J., Kirchhoff, T., del Mazo, J., Bernad, A. and Blanco, L. (2001) DNA polymerase lambda (Pol λ), a novel eukaryotic DNA polymerase with a potential role in meiosis. J. Mol. Biol., 301, 851-867.
15. Domínguez, O., Ruiz, J.F., Laín de Lera, T., García-Díaz, M., González, M.A., Kirchhoff, T., Martínez, A.C., Bernad, A. and Blanco, L. (2000) DNA polymerase mu (Pol μ), homologous to TdT, could act as a DNA mutator in eukaryotic cells. EMBO J., 19, 1731-1742.
16. Ruiz, J.F., Domínguez, O., Laín de Lera, T., García-Díaz, M., Bernad, A. and Blanco, L. (2001) DNA polymerase mu, a candidate hypermutase? Phil. Trans. R. Soc. Land. B, 356, 99-109.
17. Zhang, Y., Wu, X., Yuan, F., Xie, Z. and Wang, Z. (2001) DNA polymerase μ. Mol. Cell. Biol., 21, 7995-8006.
18. Mahajan, K.N., Nick McElhinny, S.A., Mitchell, B.S. and Ramsden, D.A. (2002) Association of DNA polymerase μ (pol μ) with ku and ligase IV: role for pol μ in end-joining double-strand break repair. Mol. Cell. Biol., 22, 5194-5202.
19. Zhang, Y., Wu, X., Guo, D., Rechkoblit, O., Taylor, J.S., Geacintov, N.E. and Wang, Z. (2002) Lesion bypass activities of human DNA polymerase μ. J. Biol. Chem., 277, 44582-44587.
20. Havener, J.M., Nick McElhinny, S.A., Bassett, E., Gauger, M., Ramsden, D.A. and Chaney, S.G. (2003) Translesion synthesis past platinum DNA adducts by human DNA polymerase μ. Biochemistry, 42, 1777-1788.
21. Pelletier, H., Sawaya, M.R., Kumar, A., Wilson, S.H. and Kraut, J. (1994) Structures of ternary complexes of rat DNA polymerase β, a DNA template-primer, and ddCTP. Science, 264, 1891-1903.
22. Delarue, M., Boulé, J.M., Lescar, J., Expert-Bezançon, N., Jourdan, N., Sukumar, N., Rougeon, F. and Papanicolaou, C. (2002) Crystal structure of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase. EMBO J., 21, 427-439.
23. Guex, N. and Peitsch, M.C. (1997) SWISS-MODEL and the Swiss-Pdb Viewer: an environment for comparative protein modeling. Electrophoresis, 18, 2714-2723.
24. Burgers, P.M.J., Koonin, E.V., Bruford, E., Blanco, L., Burtis, K.C., Christman, M.F., Copeland, W.C., Friedberg, E.C., Hanaoka, F., Hinkle, D.C. et al. (2001) Eukaryotic DNA polymerases: proposal for a revised nomenclature. J. Biol. Chem., 276, 43487-43490.
25. Bollum, F.J. (1962) Oligodeoxyribonucleotide-primed reactions catalyzed by calf thymus polymerase. J. Biol. Chem., 237, 1945-1949.
26. Kunkel, T.A. (1985) The mutational specificity of DNA polymerases-alpha and gamma during in vitro DNA synthesis. J. Biol. Chem., 260, 12866-12874.
27. García-Díaz, M., Bebenek, K., Sabariegos, R., Domínguez, O., Rodríguez, J., Kirchhoff, T., García-Palomero, E., Picher, A.J., Juárez, R., Ruiz, J.F., Kunkel, T.A. and Blanco, L. (2002) DNA polymerase λ, a novel DNA repair enzyme in human cells. J. Biol. Chem., 277, 13184-13191.
28. Meuth, M. (1984) The genetic consequences of nucleotide precursor pool imbalance in mammalian cells. Mutat. Res., 126, 107-112.
29. Oliveros, M., Yáñez, R.J., Salas, M.L., Viñuela, E. and Blanco, L. (1997) Characterization of an African swine fever virus 20-kDa DNA polymerase involved in DNA repair. J. Biol. Chem., 272, 30899-30910.
30. Nick McElhinny, S.A. and Ramsden, D.A. (2003) Polymerase mu is a DNA-directed DNA/RNA polymerase. Mol. Cell. Biol., 23, 2309-2315.
31. Han, S., Dillon, S.R., Zheng, B., Shimoda, M., Schlissel, M.S. and Kelsoe, G. (1997) V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science, 278, 301-305.
32. Mahajan, K.N., Gangi-Peterson, L., Sorscher, D.H., Wang, J., Gathy, K.N., Mahajan, N.P., Reeves, W.H. and Mitchell, B.S. (1999) Association of terminal deoxynucleotidyl transferase with Ku. Proc. Natl Acad. Sci. USA, 96, 13926-13931.