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Direct structural and functional evaluation of the types of forces acting upon MscL during gating. Single channel and EPR data suggest that changes in transbilayer pressure profiles exert a critical role in channel activation. Hydrophobic mismatch does not, on its own, trigger channel opening, although shorter bilayers generate a favorable energetic bias, reducing the activation threshold.
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This paper provides a direct spectroscopic demonstration of the helix-tilt model of MscL gating. An extensive library of spin-labeled mutants was used to derive structural information on EPR-based dynamics and solvent accessibility changes under conditions that stabilized the closed, open and an intermediate closed state. These data were used to construct models of different conformations of the TM segments along the gating pathway.
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