Modification of ribonuclease T1 specificity by random mutagenesis of the substrate binding segment

Biochemistry

Volumn 38, Issue 4, 1999, Pages 1371-1376

  Hubner, Bernd ^a,b   Haensler, Marion ^b   Hahn, Ulrich ^b  

^a UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE 2',3' PHOSPHATE; CYTIDINE; DINUCLEOSIDE PHOSPHATE; GUANINE; PURINE NUCLEOTIDE; RIBONUCLEASE T1;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ESTERIFICATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; DNA PRIMERS; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RIBONUCLEASE T1; SUBSTRATE SPECIFICITY;

EID: 0033604858     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9817515     Document Type: Article

References (45)

1. Choo, Y., and Klug, A. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 11163-11167.
2. Huang, L., Sera, T., and Schultz, P. G. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 3969-3973.
3. Hoogenboom, H., and Winter, G. (1992) J. Mol. Biol. 227, 387-381.
4. Barbas, C. F., III, Bain, J. D., Hoekstra, D. M., and Lerner, R. A. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 4457-4461.
5. Oliphant, A. R., and Struhl, K. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9094-9098.
6. Graham, L. D., Haggett, K. D., Jennings, P. A., LeBrocque, D. S., and Whittaker, R. G. (1993) Biochemistry 32, 6250-6258.
7. Viadiu, H., Osuna, J., Fink, A. L., and Soberon, X. (1995) J. Biol. Chem. 270, 781-787.
8. Hahn, U., and Heinemann, U. (1989) Concepts in Protein Engineering and Design (Wrede, P., and Schneider, G., Eds.) pp 109-168, de Gruyter, Berlin and New York.
9. Steyaert, J. (1997) Eur. J. Biochem. 247, 1-11.
10. Pace, C. N. (1990) Trends Biochem. Sci. 15, 14-17.
11. Pace, C. N., Heinemann, U., Hahn, U., and Saenger, W. (1991) Angew. Chem., Int. Ed. Engl. 30, 343-360.
12. Shirley, B. A., Stanssens, P., Hahn, U., and Pace, C. N. (1992) Biochemistry 31, 725-732.
13. Schmid, F. X., Frech, C., Scholz, C., and Walter, S. (1996) Biol. Chem. 377, 417-424.
14. Eckstein, F., Schulz, H. H., Ruterjans, H., Haar, W., and Maurer, W. (1972) Biochemistry 11, 3507-3512.
15. Heinemann, U., and Saenger, W. (1982) Nature 299, 27-31.
16. Grunert, H.-P., Zouni, A., Beineke, M., Quaas, R., Georgalis, Y., Saenger, W., and Hahn, U. (1991) Eur. J. Biochem. 197, 203-207.
17. Backmann, J., Doray, C. C., Grunert, H.-P., Landt, O., and Hahn, U. (1994) Biochem. Biophys. Res. Commun. 199, 213-219.
18. Gohda, K., Oka, K., Tomita, K., and Hakoshima, T. (1994) J. Biol. Chem. 269, 17531-17536.
19. Arni, R., Heinemann, U., Tokuoka, R., and Saenger, W. (1988) J. Biol. Chem. 263, 15358-15368.
20. Heydenreich, A., Koellner, G., Choe, H.-W., Cordes, F., Kisker, C., Schindelin, H., Adamiak, R., Hahn, U., and Saenger, W. (1993) Eur. J. Biochem. 218, 1005-1012.
21. Koepke, J., Maslowska, M., Heinemann, U., and Saenger, W. (1989) J. Mol. Biol. 206, 475-488.
22. Fushman, D., Ohlenschlager, O., and Ruterjans, H. J. (1994) J. Biomol. Struct. Dyn. 11, 1377-1402.
23. Grunert, H.-P., Landt, O., Zirpel-Giesebrecht, M., Backmann, J., Heinemann, U., Saenger, W., and Hahn, U. (1993) Protein Eng. 6, 739-744.
24. Loverix, S., Doumen, J., and Steyaert, J. (1997) J. Biol. Chem. 272, 9635-9639.
25. Hirono, S., and Kollman, P. A. (1991) Protein Eng. 4, 233-243.
26. Granzin, J., Puras-Lutzke, R., Landt, O., Grunert, H.-P., Heinemann, U., Saenger, W., and Hahn, U. (1992) J. Mol. Biol. 225, 533-542.
27. Landt, O., Grunert, H.-P., and Hahn, U. (1990) Gene 96, 125-128.
28. Quaas, R., McKeown, Y., Stanssens, P., Frank, R., Blöcker, R., and Hahn, U. (1988) Eur. J. Biochem. 173, 617-622.
29. Quaas, R., Grunert, H.-P., Kimura, M., and Hahn, U. (1988) Nucleosides Nucleotides 7, 619-623.
30. Ghrayeb, J., Kimura, H., Takahara, M., Hsiung, H., Masui, Y., and Inouye, M. (1984) EMBO J. 3, 2437-2442.
31. Inoue, H., Nojama, H., and Okayama, H. (1990) Gene 96, 23-28.
32. Quaas, R., Landt, O., Grunert, H.-P., Beineke, M., and Hahn, U. (1989) Nucleic Acids Res. 17, 3318.
33. Landt, O., Zirpel-Giesebrecht, M., Milde, A., and Hahn, U. (1992) J. Biotechnol. 24, 189-194.
34. Anfinsen, C. B., Redfield, R. R., Choate, W. L., Page, J., and Carroll, W. R. (1954) J. Biol. Chem. 207, 201-210.
35. Haensler, M., Hahn, U., and Jakubke, H.-D. (1997) Biol. Chem. 378, 115-118.
36. Kondo, N. S., Holmes, H. M., Stempel, L. M., and Ts'o, P. O. P. (1970) Biochemistry 9, 3479-3498.
37. Zabinsky, M., and Walz, F. G. (1976) Arch. Biochem. Biophys. 175, 558-564.
38. Sato, K., and Egami, F. (1957) J. Biochem. 44, 753-767.
39. Haensler, M., and Jakubke, H.-D. (1996) J. Pept. Sci. 2, 279-289.
40. Hakoshima, T., Toda, S., Sugio, S., Tomita, K., Nishikawa, S., Morioka, H., Fuchimura, K., Kimura, T., Uesugi, S., Ohtsuka, E., and Ikehara, M. (1988) Protein. Eng. 2, 55-61.
41. Hartley, R. W. (1980) J. Mol. Evol. 15, 355-358.
42. Hill, C., Dodson, G., Heinemann, U., Saenger, W., Mitsui, Y., Nakamura, K., Borisov, S., Tischenko, G., Polyakow, K., and Pavlowsky, S. (1983) Trends Biochem. Sci. 8, 364-369.
43. Walz, F. G., Jr., Osterman, H. L., and Libertin, C. (1979) Arch. Biochem. Biophys. 195, 95-102.
44. Ohgi, K., and Irie, M. (1975) J. Biochem. 77, 1085-1094.
45. Uchida, T., Arima, T., and Egami, F. (1970) J. Biochem. 67, 91-102.