Expression of recombinant proteins in a lipid A mutant of Escherichia coli BL21 with a strongly reduced capacity to induce dendritic cell activation and maturation

Journal of Immunological Methods

Volumn 272, Issue 1-2, 2003, Pages 199-210

  Cognet, Isabelle ^a   De Coignac, Amélie Benoit ^a   Magistrelli, Giovanni ^a   Jeannin, Pascale ^a   Aubry, Jean Pierre ^a   Maisnier Patin, Karine ^a   Caron, Gersende ^a   Chevalier, Sylvie ^b   Humbert, Frédéric ^a   Nguyen, Thien ^a   Beck, Alain ^a   Velin, Dominique ^a   Delneste, Yves ^a   Malissard, Martine ^a   Gauchat, Jean François ^a,c  

^a Centre d'Immunologie Pierre Fabre   (France)

^b ANGERS UNIVERSITY HOSPITAL   (France)

^c UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

E. coli BL21; Endotoxin; Homologous recombination; Lipid A; Lipopolysaccharide; Recombinant proteins

Indexed keywords

CD86 ANTIGEN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; LIPID A; LIPOPOLYSACCHARIDE; PROTEIN MD 2; RECOMBINANT PROTEIN; TOLL LIKE RECEPTOR 4; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ARTICLE; CELL ACTIVATION; CELL MATURATION; CONTAMINATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENDRITIC CELL; ESCHERICHIA COLI; GENE INACTIVATION; IMMUNE RESPONSE; MYRISTYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REDUCTION; RISK FACTOR; SALMONELLA; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

ANTIGENS, CD; ANTIGENS, CD86; ANTIGENS, SURFACE; BASE SEQUENCE; CELL DIFFERENTIATION; DENDRITIC CELLS; DNA, BACTERIAL; DROSOPHILA PROTEINS; ESCHERICHIA COLI; GENES, BACTERIAL; HUMANS; INTERLEUKIN-8; LIPID A; LYMPHOCYTE ANTIGEN 96; MEMBRANE GLYCOPROTEINS; MUTAGENESIS, INSERTIONAL; MUTATION; NF-KAPPA B; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; TOLL-LIKE RECEPTOR 4; TOLL-LIKE RECEPTORS; TUMOR NECROSIS FACTOR-ALPHA;

ESCHERICHIA COLI; SALMONELLA;

EID: 0037438154     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00506-9     Document Type: Article

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