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Volumn 285, Issue 5428, 1999, Pages 732-736

Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 12; LIPOPROTEIN; MEMBRANE RECEPTOR; NITRIC OXIDE SYNTHASE;

EID: 0033618562     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5428.732     Document Type: Article
Times cited : (1439)

References (64)
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    • Adherent monocytes were isolated as described previously (14). Cells were treated with no antibody, mouse anti-human TLR-2 neutralizing monoclonal antibody, or an isotype control mouse IgG1 (10 μg/ml) for 30 min before stimulation with LPS or 19-kD lipoprotein (50 ng/ml) and incubated for 16 hours. Supernatants were then harvested and assayed for IL-12 p40 by ELISA. CD40L- and interferon-γ (IFN-γ) - stimulated adherent monocytes were used to control for the anti-TLR-2 blocking of lipoprotein-induced IL-12. Adherent monocytes were treated with human IFN-γ (100 units/ml; Endogen, Cambridge, MA) for 16 hours to up-regulate CD40 expression. Cells were then treated with antibodies as stated above, then stimulated with soluble CD40L trimer (250 ng/ml; Immunex, Seattle, WA) for 16 hours. Neither antibody nor IFN-γ alone induced IL-12 production.
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    • We thank P. Sieling, G. Cheng, M. Mark, C. Nathan, and H. Herschman for scientific discussion; M. Gately (Hoffman-La Roche) for POD-46D; and C. Nathan (Cornell Medical College, New York, NY) for the iNOS promoter. Supported by the NIH (to R.L.M., P.J.B., B.R.B., M.V.N.), the Howard Hughes Medical Institute (to B.R.B. and S.T.S.), the United Nations Development Programme/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases (IMMLEP), and the Dermatology Research Foundation of California, Incorporated.


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