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High-affinity binding at surfaces often appears irreversible because of mass transport limited robinding. This apparent irreversibility can be reversed by washing the chip with solutions containing the probe or other molecules that bind to the sample.
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Higher coverages can be obtained depending on the amount of equilibration
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Higher coverages can be obtained depending on the amount of equilibration.
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40
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20244365554
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L = 2 nM and assume that labeling of the toxin does not influence its binding affinity
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