Patterning barriers to lateral diffusion in supported lipid bilayer membranes by blotting and stamping

Langmuir

Volumn 16, Issue 3, 2000, Pages 894-897

  Hovis, Jennifer S ^a   Boxer, Steven G ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEPOSITION; DIFFUSION; ELECTROSTATICS; HYDRATION; HYDROPHOBICITY; LIPIDS; ORGANIC POLYMERS; SURFACE TENSION; SURFACES; VAN DER WAALS FORCES;

BLOTTING; FLUID LIPID BILAYER MEMBRANES; POLYDIMETHYLSILOXANE;

BIOLOGICAL MEMBRANES;

EID: 0034620431     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la991175t     Document Type: Article

References (26)

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16. We note that the 106% expansion was seen for egg phosphatidylcholine bilayers. It is likely that the exact amount of expansion varies with different lipid compositions.
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21. We used the same lipids as in ref 14; different lipid compositions may expand to a different extent.
22. At the edges of the pattern the top and bottom leaflets must connect in some fashion to keep the hydrophobic interior away from water. The organization of the bilayer at the edges is not known; however, it is reasonable to assume that an unfavorable line energy results. This line energy should cause an isolated square section of bilayer to reorganize into a circular shape. We have observed that square grid patterns created by blotting and printed squares retain their shape for extended periods of time, suggesting that such large-scale reorganization on the surface is prevented, possibly by pinning defects on the surface.
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24. The number of barriers erased is a determined by a balance between the fractional expansion and the increased reservoir size that occurs every time a barrier is erased. This expansion does not occur indefinately. After less than ahour it terminates, resultingin the erasure of several barriers and leaving a much larger number intact.
25. In this way, structures with much greater complexity can be built up. Kung, L. A.; Hovis, J. S.; Boxer, S. G. To be submitted for publication.
26. Hovis, J. S.; Boxer, S. G. To be submitted for publication.