Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage

Nature Cell Biology

Volumn 5, Issue 3, 2003, Pages 255-260

  Lukas, Claudia ^a   Falck, Jacob ^a   Bartkova, Jirina ^a   Bartek, Jiri ^a   Lukas, Jiri ^a  

^a DANISH CANCER SOCIETY RESEARCH CENTER   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CHECKPOINT KINASE 2; DOUBLE STRANDED DNA; NIBRIN; NUCLEAR PROTEIN; PROTEIN P53; UNCLASSIFIED DRUG;

ARTICLE; ATAXIA TELANGIECTASIA; CELL CYCLE; CELL LINE; CELL NUCLEUS; CONTROLLED STUDY; DNA DAMAGE; DNA RESTRICTION; DNA STRAND BREAKAGE; DNA TRANSCRIPTION; GENE MUTATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

CELL CYCLE; CELL CYCLE PROTEINS; DNA DAMAGE; HUMANS; PHOSPHORYLATION; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 0037341599     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb945     Document Type: Article

References (30)

1. Rouse, J, & Jackson, S. P. Interface between the detection, signaling, and repair of DNA damage. Science 297, 547-551 (2002).
2. Bartek, J., Falck, J. & Lukas, J. CHK2 kinase-a busy messenger. Nature Rev. Mol. Cell Biol. 2, 877-886 (2001).
3. Limoli, C. L. & Ward, J. F. A new method for introducing double-strand breaks into cellular DNA. Radiat. Res. 13, 160-169 (1993).
4. Rogakou, E. P., Boon, C., Redon, C. & Bonner, W. M. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
5. Wang, Y. et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14, 927-939 (2000).
6. Falck, J., Petrini, J. H., Williams, B. R., Lukas, J. & Bartek, J. The DNA damage-dependent intra-S phase checkpoint is regulated by parallel pathways. Nature Genet. 30, 290-294 (2002).
7. Falck, J., Mailand, N., Syljuasen, R. G., Bartek, J. & Lukas, J. The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistent DNA synthesis. Nature 410, 842-847 (2001).
8. Ward, I. M., Wu, X. & Chen, J. Threonine 68 of Chk2 is phosphorylated at sites of DNA strand breaks. J. Biol. Chem. 276, 2971-2974 (2001).
9. Wang, B., Matsuoka, S., Carpenter, P. B. & Elledge, S. J. 53BP1, a mediator of the DNA damage checkpoint. Science 298, 1435-1438 (2002).
10. Lee, C. H. & Chung, J. H. The hCds1 (Chk2)-FHA domain is essential for a chain of phosphorylation events on hCds1 that is induced by ionizing radiation. J. Biol. Chem. 276, 30537-30541 (2001).
11. Wu, X., Webster, S. R. & Chen, J. Characterization of tumor-associated Chk2 mutations. J. Biol. Chem. 276, 47755-47758 (2001).
12. Kim, S. T., Xu, B. & Kastan, M. B. Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Genes Dev. 16, 560-570 (2002).
13. Yazdi, P. T. et al. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev. 16, 571-582 (2002).
14. D'Amours, D. & Jackson, S. P. The Mre11 complex: at the crossroads of DNA repair and checkpoint signalling. Nature Rev. Mol. Cell Biol. 3, 317-327 (2002).
15. Petrini, J. H. The Mre11 complex and ATM: collaborating to navigate S phase. Curr. Opin. Cell Biol. 12, 293-296 (2000).
16. Melo, J. A., Cohen, J. & Toczyski, D. P. Two checkpoint complexes are independently recruited to sites of DNA damage in vivo. Genes Dev. 15, 2809-2821 (2001).
17. Kanda, T., Sullivan, K. F. & Wahl, G. M. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 8, 377-385 (1998).
18. Falck, J. et al. Functional impact of concomitant versus alternative defects in the Chk2-p53 tumour suppressor pathway. Oncogene 20, 5503-5510 (2001).
19. Chehab, N. H., Malikzay, A., Appel, M. & Halazonetis, T. D. Chk2/Cds1 functions as a DNA damage checkpoint in G1 by stabilizing p53. Genes Dev. 14, 278-288 (2000).
20. Hirao, A. et al. Chk2 is a tumor suppressor that regulates apoptosis in both an ataxia telangiectasia mutated (ATM)-dependent and an ATM-independent manner. Mol. Cell. Biol. 22, 6521-6532 (2002).
21. Takai, H. et al. Chk2-deficient mice exhibit radioresistance and defective p53-mediated transcription. EMBO J. 21, 5195-5205 (2002).
22. Ahn, J. Y., Li, X., Davis, H. L. & Canman C. E. Phosphorylation of threonine 68 promotes oligomerization and autophosphorylation of the Chk2 protein kinase via the forkhead-associated domain. J. Biol. Chem. 277, 19389-19395 (2002).
23. Xu, X., Tsvetkov, L. M. & Stern, D. F. Chk2 activation and phosphorylation-dependent oligomerization. Mol. Cell. Biol. 22, 4419-4432 (2002).
24. Desai-Mehta, A., Cerosaletti, K. M. & Concannon, P. Distinct functional domains of Nibrin mediate Mre11 binding, focus formation, and nuclear localization. Mol. Cell. Biol. 21, 2184-2191 (2001).
25. Tauchi, H. et al. The forkhead-associated domain of Nbs1 is essential for nuclear foci formation after irradiation but not hRad50-hMre11-Nbs1 complex DNA repair activity. J. Biol. Chem. 276, 12-15 (2001).
26. Li, J, et al. Structural and functional versatility of the FHA domain in DNA-damage signalling by the tumor suppressor kinase Chk2. Mol. Cell 9, 1045-1054 (2002).
27. Essers, J. et al. Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage. EMBO J. 21, 2030-2037 (2002).
28. Norris, P. S. & Haas, M. A fluorescent p53GFP fusion protein facilitates its detection in mammalian cells while retaining the properties of wild-type p53. Oncogene 15, 2241-2247 (1997).
29. Stommel, J. M. et al. A leucine-rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking. EMBO J. 18, 1660-1672 (1999).
30. Lukas, C. et al. DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology. Cancer Res. 61, 4990-4993 (2001).