CDK- and Cdc45-dependent priming of the MCM complex on chromatin during S-phase in Xenopus egg extracts: Possible activation of MCM helicase by association with Cdc45

Genes to Cells

Volumn 8, Issue 2, 2003, Pages 145-161

  Masuda, Taro ^a   Mimura, Satoru ^a   Takisawa, Haruhiko ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; CYCLIN DEPENDENT KINASE; DNA; HELICASE; MINICHROMOSOME MAINTENANCE PROTEIN; PHOSPHATASE; PROTEIN CDC45; PROTEIN SUBUNIT; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CYCLE S PHASE; CHROMATIN; COMPLEX FORMATION; CONTROLLED STUDY; DNA REPLICATION; EGG; ENZYME ACTIVATION; ENZYME ACTIVITY; EUKARYOTE; IMMUNOPRECIPITATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; XENOPUS;

ANIMALS; CARRIER PROTEINS; CELL EXTRACTS; CHROMATIN; CYCLIN-DEPENDENT KINASES; DNA HELICASES; DNA REPLICATION; DNA-BINDING PROTEINS; ENZYME ACTIVATION; MODELS, BIOLOGICAL; NUCLEAR PROTEINS; OOCYTES; PHOSPHORYLATION; S PHASE; SACCHAROMYCES CEREVISIAE PROTEINS; XENOPUS;

ANIMALIA; EUKARYOTA;

EID: 0037323011     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2003.00621.x     Document Type: Article

References (45)

1. Aparicio, O.M., Weinstein, D.M. & Bell, S.P. (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91, 59-69.
2. Bell, S.P. & Dutta, A. (2002) DNA replication in eukaryotic cells. Annu. Rev. Biochem. 71, 333-374.
3. Chen, C.Y., Graham, J. & Yan, H. (2001) Evidence for a replication function of FFA-1, the Xenopus orthologue of Werner syndrome protein. J. Cell Biol. 152, 985-996.
4. Crevel, G., Ivetic, A., Ohno, K., Yamaguchi, M. & Cotterill, S. (2001) Nearest neighbour analysis of MCM protein complexes in Drosophila melanogaster. Nucl. Acids Res. 29, 4834-4842.
5. Findeisen, M., El-Denary, M., Kapitza, T., Graf, R. & Strausfeld, U. (1999) Cyclin A-dependent kinase activity affects chromatin binding of ORC, Cdc6, and MCM in egg extracts of Xenopus laevis. Eur. J. Biochem. 264, 415-426.
6. Fujita, M., Yamada, C., Tsurumi, T., Hanaoka, F., Matsuzawa, K. & Inagaki, M. (1998) Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase. J. Biol. Chem. 273, 17095-17101.
7. Hardy, C.F., Dryga, O., Seematter, S., Pahl, P.M. & Sclafani, R.A. (1997) mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. Proc. Natl. Acad. Sci. USA 94, 3151-3155.
8. Hendrickson, M., Madine, M., Dalton, S. & Gautier, J. (1996) Phosphorylation of MCM4 by cdc2 protein kinase inhibits the activity of the minichromosome maintenance complex. Proc. Natl. Acad. Sci. USA 93, 12223-12228.
9. Ishimi, Y. (1997) A DNA helicase activity is associated with an MCM4,-6, and -7 protein complex. J. Biol. Chem. 272, 24508-24513.
10. Ishimi, Y., Komamura, Y., You, Z. & Kimura, H. (1998) Biochemical function of mouse minichromosome maintenance 2 protein. J. Biol. Chem. 273, 8369-8375.
11. Ishimi, Y. & Komamura-Kohno, Y. (2001) Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4, 6, 7 helicase activity. J. Biol. Chem. 276, 34428-34433.
12. Ishimi, Y., Komamura-Kohno, Y., You, Z., Omori, A. & Kitagawa, M. (2000) Inhibition of Mcm4, 6, 7 helicase activity by phosphorylation with cyclin A/Cdk2. J. Biol. Chem. 275, 16235-16241.
13. Jares, P. & Blow, J.J. (2000) Xenopus cdc7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading. Genes Dev. 14, 1528-1540.
14. Koonin, E.V. (1993) A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Nucl. Acids Res. 21, 2541-2547.
15. Kubota, Y., Mimura, S., Nishimoto, S., Masuda, T., Nojima, H. & Takisawa, H. (1997) Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. EMBO J. 16, 3320-3331.
16. Kubota, Y., Mimura, S., Nishimoto, S., Takisawa, H. & Nojima, H. (1995) Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor. Cell 81, 601-609.
17. Kubota, Y. & Takisawa, H. (1993) Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts. J. Cell Biol. 123, 1321-1331.
18. Labib, K. & Diffley, J.F. (2001) Is the MCM2-7 complex the eukaryotic DNA replication fork helicase? Curr. Opin. Genet. Dev. 11, 64-70.
19. Labib, K., Tercero, J.A. & Diffley, J.F. (2000) Uninterrupted MCM2-7 function required for DNA replication fork progression. Science 288, 1643-1647.
20. Lee, J.K. & Hurwitz, J. (2000) Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe. J. Biol. Chem. 275, 18871-18878.
21. Lei, M., Kawasaki, Y., Young, M.R., Kihara, M., Sugino, A. & Tye, B.K. (1997) Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev. 11, 3365-3374.
22. Liao, S., Graham, J. & Yan, H. (2000) The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication. Genes Dev. 14, 2570-2575.
23. Liu, Q., Choe, W. & Campbell, J.L. (2000) Identification of the Xenopus laevis homolog of Saccharomyces cerevisiae DNA2 and its role in DNA replication. J. Biol. Chem. 275, 1615-1624.
24. Maiorano, D., Lemaitre, J.M. & Mechali, M. (2000) Stepwise regulated chromatin assembly of MCM2-7 proteins. J. Biol. Chem. 275, 8426-8431.
25. Masai, H. & Arai, K. (2002) Cdc7 kinase complex: a key regulator in the initiation of DNA replication. J. Cell. Physiol. 190, 287-296.
26. Masai, H., Matsui, E., You, Z., Ishimi, Y., Tamai, K. & Arai, K. (2000) Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 by Cdks. J. Biol. Chem. 275, 29042-29052.
27. Mimura, S., Masuda, T., Matsui, T. & Takisawa, H. (2000) Central role for Cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts. Genes Cells 5, 439-452.
28. Mimura, S. & Takisawa, H. (1998) Xenopus Cdc45-dependent loading of DNA polymerase α onto chromatin under the control of S-phase Cdk. EMBO J. 17, 5699-5707.
29. Musahl, C., Schulte, D., Burkhart, R. & Knippers, R. (1995) A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins. Eur. J. Biochem. 230, 1096-1101.
30. Nishitani, H. & Lygerou, Z. (2002) Control of DNA replication licensing in a cell cycle. Genes Cells 7, 523-534.
31. Pereverzeva, I., Whitmire, E., Khan, B. & Coue, M. (2000) Distinct phosphoisoforms of the Xenopus Mcm4 protein regulate the function of the Mcm complex. Mol. Cell. Biol. 20, 3667-3676.
32. Prokhorova, T.A. & Blow, J.J. (2000) Sequential MCM/P1 sub-complex assembly is required to form a heterohexamer with replication licensing activity. J. Biol. Chem. 275, 2491-2498.
33. Sato, M., Gotow, T., You, Z., Komamura-Kohno, Y., et al. (2000) Electron microscopic observation and single-stranded DNA binding activity of the Mcm4, 6, 7 complex. J. Mol. Biol. 300, 421-431.
34. Schulte, D., Richter, A., Burkhart, R., Musahl, C. & Knippers, R. (1996) Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins. Eur. J. Biochem. 235, 144-151.
35. Su, T.T., Feger, G. & O'Farrell, P.H. (1996) Drosophila MCM protein complexes. Mol. Biol. Cell 7, 319-329.
36. Takisawa, H., Mimura, S. & Kubota, Y. (2000) Eukaryotic DNA replication: from pre-replication complex to initiation complex. Curr. Opin. Cell. Biol. 12, 690-696.
37. Tercero, J.A., Labib, K. & Diffley, J.F. (2000) DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p. EMBO J. 19, 2082-2093.
38. Todorov, I.T., Attaran, A. & Kearsey, S.E. (1995) BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication. J. Cell Biol. 129, 1433-1445.
39. Tye, B.K. (2000) Insights into DNA replication from the third domain of life. Proc. Natl. Acad. Sci. USA 97, 2399-2401.
40. Uchiyama, M., Griffiths, D., Arai, K. & Masai, H. (2001) Essential role of Sna41/Cdc45 in loading of DNA polymerase α onto minichromosome maintenance proteins in fission yeast. J. Biol. Chem. 276, 26189-26196.
41. Walter, J.C. (2000) Evidence for sequential action of cdc7 and cdk2 protein kinases during initiation of DNA replication in Xenopus egg extracts. J. Biol. Chem. 275, 39773-39778.
42. Walter, J. & Newport, J. (2000) Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase α. Mol. Cell 5, 617-627.
43. You, Z., Komamura, Y. & Ishimi, Y. (1999) Biochemical analysis of the intrinsic Mcm4-Mcm6-Mcm7 DNA helicase activity. Mol. Cell. Biol. 19, 8003-8015.
44. Zou, L. & Stillman, B. (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280, 593-596.
45. Zou, L. & Stillman, B. (2000) Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase. Mol. Cell. Biol. 20, 3086-3096.