Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex

Journal of Molecular Biology

Volumn 300, Issue 3, 2000, Pages 421-431

  Sato, M ^a   Gotow, T ^c   You, Z ^a   Komamura Kohno, Y ^a   Uchiyama, Y ^c   Yabuta, N ^b   Nojima, H ^b   Ishimi, Y ^a  

^a MITSUBISHI KASEI INST OF LIFE SCI   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

DNA helicase; Mcm proteins; Negative staining; Single stranded DNA binding; Toroidal structure

Indexed keywords

ADENOSINE TRIPHOSPHATASE; HELICASE; MINICHROMOSOME MAINTENANCE PROTEIN KINASE; NUCLEOPROTEIN; PROTEIN KINASE; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; URANYL ACETATE; VIRUS ANTIGEN;

ARTICLE; CONTROLLED STUDY; DNA BINDING; DNA REPLICATION; ELECTRON MICROSCOPY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN STRUCTURE; SIMIAN VIRUS 40;

EID: 0034647412     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3865     Document Type: Article

References (42)

1. A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast
2. Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast
3. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46
4. Purification of an MCM-containing complex as a component of the DNA replication licensing system
5. The role of MCM/P1 proteins in the licensing of DNA replication
6. A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase
7. Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases
8. The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication
9. mcm5/cdc46-bobl Bypasses the requirement for the S phase activator Cdc7p
10. A group of interacting yeast DNA replication proteins
11. Toroidal proteins: Running rings around DNA
12. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex
13. Model system for DNA replication of a plasmid DNA containing the autonomously replicating sequence from Saccharomyces cerevisiae
14. Binding of human minichromosome maintenance proteins with histone H3
15. Biochemical function of mouse minichromosome maintenance 2 protein
16. MCM proteins: Evolution, properties, and role in DNA replication
17. The role of MCM proteins in the cell cycle control of genome duplication
18. The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum ΔH contains DNA helicase activity
19. Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: Physical interaction between P1(MCM3) and CDC46 proteins
20. Mouse MCM proteins: Complex formation and transportation to the nucleus
21. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication
22. Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor
23. Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis
24. Mechanisms of helicase-catalized DNA unwinding
25. MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells
26. Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle
27. Stepwise regulated chromatin assembly of MCM2-7 proteins
28. A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins
29. Sequential MCM/P1 subcomplex assembly is required to form a heterohexamer with replication licensing activity
30. High-molecular-mass complexes of human minichromosome-maintenance proteins in mitotic cells
31. Six molecules of SV40 large T antigen assemble in a propeller-shaped particle around a channel
32. Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: In vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
33. Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7
34. The intrinsic DNA helicase activity of Methanobacterium thermoautotrophicum uH MCM protein
35. Multiple domains of fission yeast cdcl9p (MCM2) are required for its association with the core MCM complex
36. Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity
37. Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality
38. The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides
39. MCM proteins in DNA replication
40. Cdc7p-Dbf4p kinase binds to chromatin during S phase and is regulated by both the APC and the RAD53 checkpoint pathway
41. Biochemical analysis of the intrinsic Mcm4-Mcm6-Mcm7 DNA helicase activity
42. The hexameric E. coli DnaB helicase can exist in different quarternary states