Effects of unpaired cysteines on yield, solubility and activity of different recombinant antibody constructs expressed in E. coli

Journal of Immunological Methods

Volumn 242, Issue 1-2, 2000, Pages 101-114

  Schmiedl, Andreas ^a   Breitling, Frank ^b   Winter, Christoph H ^a   Queitsch, Iris ^a   Dübel, Stefan ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Antibody engineering; Bispecific antibodies; dsFv; scFv; Streptavidin

Indexed keywords

CYSTEINE; DISULFIDE; HISTIDINE; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; RECOMBINANT ANTIBODY; STREPTAVIDIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID COMPOSITION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHEMICAL MODIFICATION; ESCHERICHIA COLI; GENETIC ENGINEERING; IMMUNOGLOBULIN VARIABLE REGION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SOLUBILITY;

AMINO ACID SEQUENCE; ANTIBODY FORMATION; ANTIGEN-ANTIBODY REACTIONS; CYSTEINE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUPPORT, NON-U.S. GOV'T;

ESCHERICHIA COLI;

EID: 0034726412     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00243-X     Document Type: Article

References (48)

1. Adams G.P., McCartney J.E., Tai M.-S., Oppermann H., Huston J.S., Stafford W.F., Bookman M.A., Fand I., Houston L.L., Weiner L.M. Highly specific in vivo tumor targeting by monovalent and divalent forms of 741F8 anti-c-erb B-2 single-chain Fv. Cancer Res. 53:1993;4026.
2. Alberini C.M., Bet P., Milstein C., Sitia R. Secretion of immunoglobulin M assembly intermediates in the presence of reducing agents. Nature. 347:1990;485.
3. Bauskin A.R., Alkalay I., Ben-Neriah Y. Redox regulation of a protein tyrosine kinase in the endoplasmic reticulum. Cell. 66:1991;685.
4. 2. Proc. Natl. Acad. Sci. USA. 90:1993;457.
5. Bird R.E., Hardman K.D., Jacobson J.W., Johnson S., Kaufman B.M., Lee S.-M., et al. Single-chain antigen-binding proteins. Science. 242:1988;423.
6. Breitling F., Dübel S. Cloning and expression of single chain fragments (scFv) from mouse and rat hybridomas. Reischl U. Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine. Vol. 13:1997;Humana Press, Totowa, NY.
7. Breitling F., Dübel S. Recombinant Antibodies. 2000;John Wiley and Sons, New York.
8. Brinkmann U., Gallo M., Brinkmann E., Kunwar S., Pastan I. A recombinant immunotoxin that is active on prostate cancer cells and that is composed of the Fv region of monoclonal antibody PR1 and a truncated form of Pseudomonas exotoxin. Proc. Natl. Acad. Sci. USA. 90:1993;7538.
9. Carter P., Kelley R.F., Rodrigues M.L., Snedecor B., Covarrubias M., Velligan M., et al. High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Biotech. 10:1992;163.
10. Colcher D., Pavlinkova G., Beresford G., Booth B.J., Batra S.K. Single-chain antibodies in pancreatic cancer. Ann. NY Acad. Sci. 880:1999;263.
11. Collesi C., Santoro M.M., Gaudino G., Comoglio P.M. A splicing variant of the RON transcript induces constitutive tyrosine kinase activity and an invasive phenotype. Mol. Cell Biol. 16:1996;5518.
12. Cumber A.J., Ward E.S., Winter G., Parnell G., Wawrzynczak E.J. Comparative stabilities in vitro and in vivo of a recombinant mouse antibody FvCys fragment and a bisFvCys conjugate. J. Immun. 149:1992;120.
13. Debinski W., Pastan I. Recombinant F(ab′) C242-Pseudomonas exotoxin, but not the whole antibody-based immunotoxin, causes regression of a human colorectal tumor xenograft. Clin. Cancer Res. 1:(9):1995;1015.
14. Dübel S., Breitling F., Fuchs P., Klewinghaus I., Little M. A family of vectors for surface display and production of antibodies. Gene. 128:1993;97.
15. Dübel S., Breitling F., Klewinghaus I., Little M. Regulated secretion and purification of recombinant antibodies in E. coli. Cell. Biophys. 21:1992;69.
16. Dübel S., Breitling F., Kontermann R., Schmidt T., Skerra A., Little M. Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). J. Immunol. Meth. 178:1995;201.
17. Esser V., Russell D.W. Transport-deficient mutations in the low density lipoprotein receptor. Alterations in the cysteine-rich and cysteine-poor regions of the protein block intracellular transport. J. Biol. Chem. 263:1988;13276.
18. Evan G.I., Lewis G.K., Ramsay G., Bishop J.M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell Biol. 5:1985;3610.
19. Fra A.M., Fagioli C., Finazzi D., Sitia R., Alberini C.M. Quality control of ER synthesized proteins: an exposed thiol group as a three-way switch mediating assembly, retention and degradation. EMBO J. 12:1993;4755.
20. Friedman P.N., Chace D.F., Trail P.A., Siegall C.B. Antitumor activity of a single-chain immunotoxin BR96 sFv-PE40 against established breast and lung tumor xenografts. J. Immun. 150:1993;3054.
21. Goshorn S.C., Svensson H.P., Kerr D.E., Somerville J.E., Senter P.D., Fell H.P. Genetic construction, expression, and characterization of a single-chain anti-carcinoma antibody fused to β-lactamase. Cancer Res. 53:1993;2123.
22. Huston J.S., Levinson D., Mudgett-Hunter M., Tai M.-S., Novotny J., Margolies M.N., et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA. 85:1988;5879.
23. Kerem A., Kronman C., Bar-Nun S., Shafferman A., Velan B. Interrelations between assembly and secretion of recombinant human acetylcholinesterase. J. Biol. Chem. 268:1993;180.
24. Kipriyanov S.M., Dübel S., Breitling F., Kontermann R.E., Heymann S., Little M. Bacterial expression and refolding of single-chain Fv fragments with C-terminal cysteines. Cell. Biophys. 26:1995;187-204.
25. Kipriyanov S., Dübel S., Breitling F., Kontermann R.E., Little M. Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: ]Production of bivalent and biotinylated mini-antibodies. Molec. Immun. 31:1994;1047.
26. Kontermann R.E., Liu Z., Schulze R.A., Sommer K.A., Queitsch I., Dübel S., et al. Characterization of the epitope recognised by a monoclonal antibody directed against the largest subunit of Drosophila RNA polymerase II. Biol. Chem. Hoppe-Seyler. 376:1995;473.
27. Krämer A., Haars R., Kabisch R., Will H., Bautz F.A., Bautz E.K.F. Monoclonal Antibody Directed Against RNA Polymerase II of Drosophila melanogaster. Molec. gen. Genet. 180:1980;193.
28. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature. 227:1970;680.
29. Lei S.-P., Lin H.-C., Wang S.-S., Callaway J., Wilcox G. Characterization of the Erwinia carotovora pelB gene and its product pectate lyase. J. Bacteriol. 169:1987;4379.
30. Li J.Y., Sugimura K., Boado R.J., Lee H.J., Zhang C., Dübel S., et al. Genetically engineered brain drug delivery vectors: cloning, expression, and in vivo application of an anti-transferrin receptor single chain antibody-streptavidin fusion gene and protein. Protein Eng. 12:1999;787.
31. Liberatore M., Neri D., Neri G., Pini A., Iurilli A.P., Ponzo F., et al. Efficient one-step direct labelling of recombinant antibodies with technetium-99m. Eur. J. Nucl. Med. 22:1995;1326.
32. Lilius G., Person M., Bülow L., Mosbach K. Metal affinity precipitation of proteins carrying genetically attached polyhistidine tails. Eur. J. Biochem. 198:1991;499.
33. Marks J.D., Griffiths A.D., Malmqvist M., Clackson T.P., Bye J.M., Winter G. By-passing immunization: building high affinity human antibodies by chain shuffling. Biotech. 10:1992;779.
34. Milenic D.E., Yokota T., Filpula D.R., Finkelman M.A.J., Dodd S.W., Wood J.F., et al. Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51:1991;6363.
35. Olson T.S., Lane M.D. A common mechanism for posttranslational activation of plasma membrane receptors? FASEB J. 3:1989;1618.
36. Reiter Y., Brinkmann U., Jung S.H., Pastan I., Lee B. Disulfide stabilization of antibody Fv: computer predictions and experimental evaluation. Protein Eng. 8:1995;1323.
37. 2 formation in Escherichia coli and for improved in vitro stability. J. Immun. 151:1993;6954.
38. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning. A Laboratory Manual. 2nd ed :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
39. Sitia R., Neuberger M., Alberini C., Bet P., Fra A., Valetti C., et al. Developmental regulation of IgM secretion: the role of the carboxy-terminal cysteine. Cell. 60:1990;781.
40. Towbin H., Staehelin T., Gordon I. Electrophoretic transfer of protein from polyacrylamid gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. USA. 76:1980;4350.
41. Verhaar M.J., Keep P.A., Hawkins R.E., Robson L., Casey J.L., Pedley B., et al. Technetium-99m radiolabeling using a phage-derived single-chain Fv with a C-terminal cysteine. Nucl. Med. 37:1996;868.
42. Waibel R., Alberto R., Willuda J., Finnern R., Schibli R., Stichelberger A., et al. Stable one-step technetium-99m labeling of his-tagged recombinant proteins with a novel Tc(I)-carbonyl complex. Nat. Biotechnol. 17:1999;897.
43. Wang D., Li Q., Hudson W., Berven E., Uckun F., Kersey J.H. Generation and characterization of an anti-CD19 single-chain Fv immunotoxin composed of C-terminal disulfide-linked dgRTA. Bioconjug. Chem. 8:1997;878.
44. Whitlow M., Fipula D. Single-chain Fv proteins and their fusion proteins. Methods: A Companion to Meth. Enzym. 2:1991;97.
45. Wunderlich M., Glockshuber R. Redox properties of protein disulfide isomerase (DsbA) from Escherichia coli. Protein Sci. 2:1993;717.
46. Wunderlich M., Glockshuber R. In vivo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). J. Biol. Chem. 268:1993;24547.
47. Yamamoto T., Bishop R.W., Brown M.S., Goldstein J.L., Russell D.W. Deletion in cysteine-rich region of LDL receptor impedes transport to cell surface in WHHL rabbit. Science. 232:1986;1230.
48. Yokota T., Milenic D.E., Whitlow M., Schlom J. Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 52:1992;3402.