proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermenter production of a recombinant antibody fragment

Gene

Volumn 274, Issue 1-2, 2001, Pages 111-118

  Fiedler, Markus ^a   Skerra, Arne ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

E. coli expression; Immunoglobulin; Metabolic selection; Monoclonal antibody IN 1; Protein engineering

Indexed keywords

CHLORAMPHENICOL; IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT ANTIBODY; RECOMBINANT DNA; RECOMBINANT PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; AUXOTROPHY; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; BIOREACTOR; CHROMOSOME DELETION; CONTROLLED STUDY; DNA VECTOR; ESCHERICHIA COLI; FERMENTATION; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENETIC COMPLEMENTATION; GENETIC STABILITY; GENETIC TRANSCRIPTION; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECRETION; VIRUS RECOMBINANT;

ALDEHYDE OXIDOREDUCTASES; CELL DIVISION; CLONING, MOLECULAR; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; GENETIC MARKERS; GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN FRAGMENTS; MUTATION; OPERON; PHOSPHOTRANSFERASES (CARBOXYL GROUP ACCEPTOR); PLASMIDS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; PROBA;

EID: 0035934280     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00629-1     Document Type: Article

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