메뉴 건너뛰기




Volumn 297, Issue 5, 2000, Pages 1105-1120

A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin

Author keywords

Affinity maturation; Alkaline phosphatase; Anticalin; Colony screening; Phage display

Indexed keywords

ALKALINE PHOSPHATASE; BILIN BINDING PROTEIN; DIGOXIGENIN; LIPOCALIN; RECEPTOR PROTEIN; UNCLASSIFIED DRUG;

EID: 0034646561     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3646     Document Type: Article
Times cited : (102)

References (56)
  • 11
    • 0022539027 scopus 로고
    • Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris-buffer system without urea
    • (1986) Anal. Biochem. , vol.155 , pp. 83-88
    • Fling, S.P.1    Gregerson, D.S.2
  • 13
    • 0007905239 scopus 로고
    • Studies on the Epstein-Barr virus genome. PhD Thesis, Cambridge University, England
    • (1984)
    • Gibson, T.J.1
  • 28
    • 0032170797 scopus 로고    scopus 로고
    • Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates
    • (1998) J. Immunol. Methods , vol.218 , pp. 73-83
    • Konig, T.1    Skerra, A.2
  • 34
    • 0007899651 scopus 로고
    • A Short Course in Bacterial Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
    • (1992)
    • Miller, J.H.1
  • 37
    • 0027156152 scopus 로고
    • Functional expression of the uncomplexed serum retinol-binding protein in Escherichia coli: Ligand binding and reversible unfolding characteristics
    • (1993) J. Mol. Biol. , vol.230 , pp. 725-732
    • Muller, H.N.1    Skerra, A.2
  • 43
    • 0031879320 scopus 로고    scopus 로고
    • Improved folding of apo-retinol-binding protein in the periplasm of E. coli: Positive influences of dsbC coexpression and of an amino acid exchange in the vitamin A binding site
    • (1998) Protein Eng. , vol.11 , pp. 601-607
    • Schmidt, A.M.1    Bloss, I.2    Skerra, A.3
  • 44
    • 0028044571 scopus 로고
    • The bilin-binding protein of Pieris brassicae: cDNA sequence and regulation of expression reveal distinct features of this insect pigment protein
    • (1994) Eur. J. Biochem. , vol.219 , pp. 855-863
    • Schmidt, F.S.1    Skerra, A.2
  • 45
    • 0027499708 scopus 로고
    • The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment
    • (1993) Protein Eng. , vol.6 , pp. 109-122
    • Schmidt, T.G.M.1    Skerra, A.2
  • 48
  • 49
    • 0026718980 scopus 로고
    • Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity
    • (1992) Nucl. Acids Res. , vol.20 , pp. 3551-3554
    • Skerra, A.1
  • 50
    • 0028555357 scopus 로고
    • Use of the tetracycline promoter for the tightly regulated production of a murine anti body fragment in Escherichia coli
    • (1994) Gene , vol.151 , pp. 131-135
    • Skerra, A.1
  • 51
    • 0025783791 scopus 로고
    • Filter screening of antibody Fab fragments secreted from individual bacterial colonies: Specific detection of antigen, binding with a two-membrane system
    • (1991) Anal. Biochem. , vol.196 , pp. 151-155
    • Skerra, A.1    Dreher, M.L.2    Winter, G.3
  • 54
    • 0030780364 scopus 로고    scopus 로고
    • Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification
    • (1997) Protein Eng. , vol.10 , pp. 975-982
    • Voss, S.1    Skerra, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.