The relative amount of cRNA coding for γ 2 subunits affects stimulation by benzodiazepines in GABAA receptors expressed in Xenopus oocytes

Neuropharmacology

Volumn 43, Issue 4, 2002, Pages 695-700

  Boileau, A J ^a   Baur, R ^b   Sharkey, L M ^a   Sigel, Erwin ^b   Czajkowski, C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Benzodiazepines; Diazepam; Functional expression; GABAA receptors; Xenopus oocyte

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; BENZODIAZEPINE; COMPLEMENTARY RNA; DRUG RECEPTOR; RECOMBINANT PROTEIN;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CHLORIDE CURRENT; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG POTENTIATION; EMBRYO; GENE EXPRESSION; GENETIC CODE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; RAT; XENOPUS;

ANIMALS; BENZODIAZEPINES; CELL LINE; ELECTROPHYSIOLOGY; GABA MODULATORS; HUMANS; MEMBRANE POTENTIALS; OOCYTES; PATCH-CLAMP TECHNIQUES; RECEPTORS, GABA-A; RNA, COMPLEMENTARY; STIMULATION, CHEMICAL; XENOPUS;

EID: 0036753758     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(02)00036-9     Document Type: Article

References (41)

1. Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J. GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. Nat. Neurosci. 4:2001;908-916.
2. Baumann S.W., Baur R., Sigel E. Subunit arrangement of gamma-aminobutyric acid type A receptors. J. Biol. Chem. 276:2001;36275-36280.
3. A receptors display association of gamma 2-subunit with alpha 1- and beta 2/3-subunits. J. Biol. Chem. 266:1991;4478-4483.
4. Benke D., Fritschy J.M., Trzeciak A., Bannwarth W., Mohler H. Distribution, prevalence, and drug binding profile of gamma-aminobutyric acid type A receptor subtypes differing in the beta-subunit variant. J. Biol. Chem. 269:1994;27100-27107.
5. Boileau A.J., Czajkowski C. Identification of transduction elements for benzodiazepine modulation of the GABA(A) receptor: three residues are required for allosteric coupling. J. Neurosci. 19:1999;10213-10220.
6. Boileau A.J., Kucken A.M., Evers A.R., Czajkowski C. Molecular dissection of benzodiazepine binding and allosteric coupling using chimeric gamma-aminobutyric acid type A receptor subunits. Mol. Pharmacol. 53:1998;295-303.
7. Buhr A., Sigel E. A point mutation in the gamma2 subunit of gamma-aminobutyric acid type A receptors results in altered benzodiazepine binding site specificity. Proc. Natl. Acad. Sci. USA. 94:1997;8824-8829.
8. Buhr A., Schaerer M.T., Baur R., Sigel E. Residues at positions 206 and 209 of the alpha1 subunit of gamma-aminobutyric AcidA receptors influence affinities for benzodiazepine binding site ligands. Mol. Pharmacol. 52:1997;676-682.
9. A receptor. J. Neurosci. 16:1996;5415-5424.
10. A receptor clustering and modulates the channel kinetics. Proc. Natl. Acad. Sci. USA. 97:2000;11557-11562.
11. Connolly C.N., Uren J.M., Thomas P., Gorrie G.H., Gibson A., Smart T.G., Moss S.J. Subcellular localization and endocytosis of homomeric gamma2 subunit splice variants of gamma-aminobutyric acid type A receptors. Mol. Cell. Neurosci. 13:1999;259-271.
12. 2+. Neuron. 5:1990;781-788.
13. A receptor subtypes requires the gamma 2 subunit and gephyrin. Nat. Neurosci. 1:1998;563-571.
14. Farrar S.J., Whiting P.J., Bonnert T.P., McKernan R.M. Stoichiometry of a ligand-gated ion channel determined by fluorescence energy transfer. J. Biol. Chem. 274:1999;10100-10104.
15. A receptors: an allosteric, state- dependent mechanism determined by the gamma-subunit. J. Physiol. 506:1998;609-625.
16. A receptor subunit requires a monomeric subunit of alpha 6 or gamma 2. J. Biol. Chem. 270:1995;26063-26066.
17. Krieg P.A., Melton D.A. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Research. 12:1984;7057-7070.
18. Krieg P.A., Melton D.A. In vitro RNA synthesis with SP6 RNA polymerase. Methods Enzymol. 155:1987;397-415.
19. A receptor subunit mRNAs in the rat brain. II. Olfactory bulb and cerebellum. J. Neurosci. 12:1992;1063-1076.
20. A receptor microscopic affinity. Neuropharmacology. 35:1996;1383-1392.
21. Liman E.R., Tytgat J., Hess P. Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs. Neuron. 9:1992;861-871.
22. A receptor. FEBS Lett. 246:1989;145-148.
23. A-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands. Brain Res. Mol. Brain Res. 8:1990;199-208.
24. A receptor. J. Neurosci. 10:1990;2330-2337.
25. A receptors in cultured murine cerebellar granule cells. J. Physiol. (Lond.). 503:1997;353-369.
26. Pajor A.M. Sequence and functional characterization of a renal sodium/dicarboxylate cotransporter. J, Biol. Chem. 270:1995;5779-5785.
27. A receptors in central synapses. J. Neurosci. 19:1999;578-588.
28. A receptor subunit for benzodiazepine pharmacology. Nature. 338:1989;582-585.
29. Robertson G.A., Warmke J.M., Ganetzky B. Potassium currents expressed from Drosophila and mouse eag cDNAs in Xenopus oocytes. Neuropharmacology. 35:1996;841-850.
30. Sigel E. Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid. J. Physiol. 386:1987;73-90.
31. A receptors. Trends Pharmacol. Sci. 18:1997;425-429.
32. Sigel E., Baur R. Allosteric modulation by benzodiazepine receptor ligands of the GABAA receptor channel expressed in Xenopus oocytes. J. Neurosci. 8:1988;289-295.
33. A) receptor. J. Neurochem. 74:2000;2590-2596.
34. A receptors on channel function. Neuron. 5:1990;703-711.
35. A receptors. Biochem. J. 310:1995;1-9.
36. A receptor: structural rearrangements detected during channel gating. J. Neurosci. 21:2001;4977-4986.
37. A receptor subtype. J. Neurosci. 17:1997;2728-2737.
38. Wagner D.A., Czajkowski C. Structure and dynamics of the GABA binding pocket: A narrowing cleft that constricts during activation. J. Neurosci. 21:2001;67-74.
39. A receptors via two distinct and separable mechanisms. Nat. Neurosci. 3:2000;1274-1281.
40. Yisraeli J.K., Melton D.A. Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases. Methods Enzymol. 180:1989;42-50.
41. Zhang L., Weiner J.L., Carlen P.L. Potentiation of gamma-aminobutyric acid type A receptor-mediated synaptic currents by pentobarbital and diazepam in immature hippocampal CA1 neurons. J. Pharmacol. Exp. Ther. 266:1993;1227-1235.