Electrophysiological evidence for the coexistence of α1 and α6 subunits in a single functional GABA(A) receptor

Journal of Neurochemistry

Volumn 74, Issue 6, 2000, Pages 2590-2596

  Sigel, Erwin ^a   Baur, Roland ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Furosemide; Recombinant DNA; Transient expression; Xenopus oocytes

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; DIAZEPAM; FLUMAZENIL; FUROSEMIDE; GLYCINE RECEPTOR; NICOTINIC RECEPTOR; SEROTONIN RECEPTOR;

ALLOSTERISM; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BRAIN ELECTROPHYSIOLOGY; DOSE RESPONSE; IMMUNOCYTOCHEMISTRY; NONHUMAN; OOCYTE; PRIORITY JOURNAL; RECEPTOR BINDING; XENOPUS;

ALLOSTERIC SITE; ANIMALS; BENZODIAZEPINES; CELLS, CULTURED; CEREBELLUM; DIURETICS; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHYSIOLOGY; FLUMAZENIL; FUROSEMIDE; GABA MODULATORS; GAMMA-AMINOBUTYRIC ACID; GENE EXPRESSION; LIGANDS; MEMBRANE POTENTIALS; NEURONS; OOCYTES; RECEPTORS, GABA-A; RECOMBINANT PROTEINS; XENOPUS LAEVIS;

ANIMALIA;

EID: 0034106074     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2000.0742590.x     Document Type: Article

References (43)

1. A receptors containing both α1 and α3 subunits. J. Biol. Chem. 271, 27902-27911.
2. A receptor deduced from mutation-induced rectification. Neuroreport 13, 285-288.
3. A receptors: classification on the basis of subunit structure and receptor function. Pharmacol. Rev. 50, 291-313.
4. A receptors display association of gamma 2-subunit with alpha 1- and beta 2/3-subunits. J. Biol. Chem. 266, 4478-4483.
5. Bonnert T. P., McKernan R. M., Farrar S., Le Bourdellès B., Heavens R. P., Smith D. W., Hewson L., Rigby M. R., Sirinathsinghji D. J. S., Brown N., Wafford K. A., and Whiting P. J. (1999) θ, a novel γ-aminobutyric acid type A receptor subunit. Proc. Natl. Acad. Sci. USA 96, 9891-9896.
6. Buhr A. and Sigel E. (1997) A point mutation in the γ2 subunit of γ-aminobutyric acid type A receptors results in altered benzodiazepine site specificity. Proc. Natl. Acad. Sci. USA 94, 8824-8829.
7. A receptor. J. Neurosci. 16, 5415-5424.
8. A receptor. J. Neurochem. 70, 2188-2194.
9. A receptor subunit. Nature 385, 820-823.
10. Fritschy J. M., Benke D., Mertens S., Oertel W. H., Bachi T., and Möhler H. (1992) Five subtypes of type A gamma-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunit-specific antibodies. Proc. Natl. Acad. Sci. USA 89, 6726-6730.
11. Galzi J.-L. and Changeux J. P. (1994) Neurotransmitter-gated ion channels as unconventional allosteric proteins. Curr. Opin. Struct. Biol. 4, 554-565.
12. A receptor subunit in tissues of the reproductive system. J. Biol. Chem. 272, 15346-15350.
13. A receptor subunit requires a monomeric subunit of α6, or γ2. J. Biol. Chem. 270, 26063-26066.
14. A receptors containing α6 subunits. J. Neurosci. 18, 2449-2457.
15. A receptor maintaining their individual benzodiazepine-binding specificities. J. Neurochem. 66, 685-691.
16. Korpi E. R., Kuner T., Seeburg P. H., and Lüddens H. (1995) Selective antagonist for the cerebellar granule cell-specific γ-aminobutyric acid type A receptor. Mol. Pharmacol. 47, 283-289.
17. A receptor subunit mRNAs in the rat brain. II. Olfactory bulb and cerebellum. J. Neurosci. 12, 1063-1076.
18. Li M. and de Blas A. L. (1997) Coexistence of two β subunit isoforms in the same γ-aminobutyric acid type A receptor. J. Biol. Chem. 272, 16564-16569.
19. A receptor. FEBS Lett. 246, 145-148.
20. A receptor selective for a behavioural alcohol antagonist. Nature 346, 648-651.
21. A receptor channels. Annu. Rev. Neurosci. 17, 569-602.
22. A-receptor expressed from rat brain α- and β-subunit cDNAs display potentiation by benzodiazepine receptor ligands. Mol. Brain Res. 8, 199-208.
23. A receptor. J. Neurosci. 10, 2330-2337.
24. A receptor structure and physiological properties in cultured cerebellar granule cells. Neuron 13, 149-158.
25. A-receptor subtypes really occur in the brain? Trends Neurosci. 19, 139-143.
26. A receptors in cultured murine cerebellar granule cells. J. Physiol. (Lond.) 503, 353-369.
27. A receptor is concentrated in both inhibitory and excitatory on cerebellar granule cells. J. Neurosci. 16, 103-114.
28. Nusser Z., Sieghart W., and Somogyi P. (1998) Segregation of different GABAA receptors to synaptic and extrasynaptic membranes of cerebellar granule cells. J. Neurosci. 18, 1693-1703.
29. A receptor subpopulations. J. Biol. Chem. 268, 3753-3757.
30. A receptors of adult rat cerebellum demonstrates two α subunits per receptor oligomer. J. Biol. Chem. 270, 21285-21290.
31. Pritchett D. B. and Seeburg P. H. (1991) γ-Aminobutyric acid type A receptor point mutation increases the affinity of compounds for the benzodiazepine site. Proc. Natl. Acad. Sci. USA 88, 1421-1425.
32. A receptor research. Synapse 21, 189-274.
33. Schofield P. R., Darlison M. G., Fujita N., Burt D. R., Stephenson F. A., Rodriguez H., Rhee L. M., Ramachandran J., Reale V., Glencorse T. A., Seeburg P. H., and Barnard E. A. (1987) Sequence and functional expression of the GABA-A receptor shows a ligand-gated receptor superfamily. Nature 328, 221-227.
34. A receptor subtypes. Pharmacol. Rev. 47, 181-233.
35. Sigel E. (1987) Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid. J. Physiol. (Lond.) 386, 73-90.
36. A, receptors. Trends Phamacol. Sci. 18, 425-429.
37. Sigel E., Stephenson F. A., Mamalaki C., and Barnard E. A. (1983) A γ-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex, purification and partial characterization. J. Biol. Chem. 258, 6965-6971.
38. A receptors on channel function. Neuron 5, 703-711.
39. Somogyi P., Fritschy J. M., Benke D., Roberts J. D., and Sieghart W. (1996) The gamma 2 subunit of the GABAA receptor is concentrated in synaptic junctions containing the alpha 1 and beta 2/3 subunits in hippocampus, cerebellum and globus pallidus. Neuropharmacology 35, 1425-1444.
40. Thompson S. A., Arden S. A., Marshall G., Wingrove P. B., Whiting P. J., and Wafford K. A. (1999) Residues in transmembrane domains I and II determine γ-aminobutyric acid type A receptor subtype-selective antagonism by furosemide. Mol. Pharmacol. 55, 993-999.
41. A receptor α6 subunit. J. Neurosci. 16, 3630-3640.
42. A receptor subtype. J. Neurosci. 17, 2728-2737.
43. A receptors is essential for benzodiazepine agonist binding. J. Biol. Chem. 267, 1426-1429.