Manipulating gene expressions by electroporation in the developing brain of mammalian embryos

Differentiation

Volumn 70, Issue 4-5, 2002, Pages 155-162

  Takahashi, Masanori ^a   Sato, Kenichi ^a   Nomura, Tadashi ^a   Osumi, Noriko ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Electroporation; in utero manipulation; Mammalian brain; Ultrasound guided manipulation; Whole embryo culture

Indexed keywords

ANIMAL TISSUE; ARTICLE; BRAIN DEVELOPMENT; CONTROLLED STUDY; ELECTROPORATION; EMBRYO; EMBRYO CULTURE; FETUS DEVELOPMENT; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE INTERACTION; GENE MUTATION; GENE TECHNOLOGY; GENE TRANSFER; GENETIC ANALYSIS; GENETIC MANIPULATION; IMAGING SYSTEM; MAMMAL; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; ULTRASOUND; ANIMAL; BRAIN; CULTURE TECHNIQUE; DRUG EFFECT; ECHOGRAPHY; FEMALE; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; MUTATION; PHYSIOLOGY; PREGNANCY; PRENATAL DEVELOPMENT; RHOMBENCEPHALON;

ANIMALIA; MAMMALIA; VERTEBRATA;

ANTISENSE OLIGONUCLEOTIDE; EYE PROTEIN; GREEN FLUORESCENT PROTEIN; HOMEODOMAIN PROTEIN; HU ANTIGEN; HU NEURONAL RNA BINDING PROTEINS; HU NEURONAL RNA-BINDING PROTEINS; NKX 2.2 HOMEDOMAIN PROTEIN; NKX-2.2 HOMEDOMAIN PROTEIN; PAIRED BOX TRANSCRIPTION FACTOR; PHOTOPROTEIN; REPRESSOR PROTEIN; RNA BINDING PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PAX6;

ANIMALS; BRAIN; CULTURE TECHNIQUES; ELECTROPORATION; EMBRYO; EYE PROTEINS; FEMALE; GENE EXPRESSION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; HU PARANEOPLASTIC ENCEPHALOMYELITIS ANTIGENS; LUMINESCENT PROTEINS; MICE; MUTATION; OLIGONUCLEOTIDES, ANTISENSE; PAIRED BOX TRANSCRIPTION FACTORS; PREGNANCY; RATS; REPRESSOR PROTEINS; RHOMBENCEPHALON; RNA-BINDING PROTEINS; TRANSCRIPTION FACTORS; ULTRASONOGRAPHY, PRENATAL;

EID: 0036595981     PISSN: 03014681     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-0436.2002.700405.x     Document Type: Review

References (58)

1. Akamatsu, W., Okano, H.J., Osumi, N., Inoue, T., Nakamura, S., Sakakibara, S., Miura, M., Matsuo, N., Darnell, R.B. and Okano, H. (1999) Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems. Proc Natl Acad Sci USA 96:9885-9890.
2. Augustine, K., Liu, E.T. and Sadler, T.W. (1993) Antisense attenuation of Wnt-1 and Wn-3a expression in whole embryo culture reveals roles for these genes in craniofacial, spinal cord, and cardiac morphogenesis. Develop Genet 14:500-520.
3. Briscoe, J., Pierani, A., Jessell, T.M. and Ericson, J. (2000) A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube. Cell 101:435-445.
4. + interneurons that require PAX6 for their development. Development 124:4493-4503.
5. Cockroft, D.L. (1990) Do section and culture of post-implantation embrups. In: Copp, A.J. and Cockroft, D.L. (eds) Mammalian Postimplantation Embryos: A Practical Approach. IRL, Press, Oxford, pp 15-40.
6. Ekker, S.C. and Larson, J.D. (2001) Morphant technology in model developmental systems. Genesis 30:89-93.
7. Endo, Y., Osumi, N. and Wakamatsu, Y. (2002) Bimodal functions of Notch-mediated signaling are involved in neural crest formation during avian ectoderm development. Development 129:863-873.
8. Ericson, J., Rashbass, P., Schedl, A., Brenner-Morton, S., Kawakami, A., van Heyningen, V., Jessell, T.M. and Briscoe, J. (1997) Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell 90:169-180.
9. Fukuchi-Shimogori, T. and Grove, E.A. (2001) Neocortex patterning by the secreted signaling molecule FGF8. Science 294:1071-1074.
10. Gaiano, N., Kohtz, J.D., Turnbull, D.H. and Fishell, G. (2000) A method for rapid gain-of-function studies in the mouse embryonic nervous system. Nat Neurosci 2:812-819.
11. Heasman, J., Holwill, S. and Wylie, C.C. (1991) Fertilization of cultured Xenopus oocytes and use in studies of maternally inherited molecules. Methods Cell Biol 36:213-230.
12. Heasman, J., Ginsberg, D., Geiger, B., Goldstone, K., Pratt, T., Yoshida-Noro, C. and Wylie, C. (1994) A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage. Development 120:49-57.
13. Heasman, J., Kofron, M. and Wylie, C. (2000) Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach. Dev Biol 222:124-134.
14. Heasman, J. (2002) Morpholino oligos: making sense of antisense? Dev Biol 243:209-214.
15. Howard, E.W., Newman, L.A., Oleksyn, D.W., Angerer, R.C. and Angerer, L.M. (2001) SpKr1: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128:365-375.
16. Inoue, T. and Krumlauf, R. (2001) An impulse to the brain-using in vivo electroporation. Nat Neurosci 4(Suppl):1156-1158.
17. Inoue, T., Tanaka, T., Takeichi, M., Chisaka, O., Nakamura, S. and Osumi, N. (2001a) Role of cadherins in maintaining the compartment boundary between the cortex and striatum during development. Development 128:561-569.
18. Inoue, T., Nakamura, S. and Osumi, N. (2001b) Current topics in comparative developmental biology of vertebrate brains. Neurosci Res 39:371-376.
19. Itasaki, N., Bel-Vialar, S. and Krumlauf, R. (1999) 'Shocking' developments in chick embryology: electroporation and in ovo gene expression. Nat Cell Biol 1:203-207.
20. Kageyama, R., Ishibashi, M. and Moriyoshi, K. (1996) Gene delivery to the nervous system by direct injection of retrovial vector. In: Latchman (ed) Genetic manipulation of the nervous system. Academic Press, London, pp 135-148.
21. Kinder, S.J., Tan, S.S. and Tam, P.P. (2000) Cell grafting and fate mapping of the early-somite-stage mouse embryo. Methods Mol Biol 135:425-437.
22. Kos, R., Reedy, M.V., Johnson, R.L. and Erickson, C.A. (2001) The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos. Development 128:1467-1479.
23. La Vail, J.H. and Cowan, W.M. (1971) The development of the chick optic tectum. I. Normal morphology and cytoarchitectonic development. Brain Res 28:391-419.
24. Liu, A., Joyner, A.L. and Turnbull, D.H. (1998) Alteration of limb and brain patterning in early mouse embryos by ultrasound-guided injection of Shh-expressing cells. Mech Dev 75:107-115.
25. Mastick, G.S. and Andrews, G.L. (2001) Pax6 regulates the identity of embryonic diencephalic neurons. Mol Cell Neurosci 17:190-207.
26. Matsuo, T., Osumi-Yamashita, N., Noji, S., Ohuchi, H., Koyama, E., Myokai, F., Matsuo, N., Taniguchi, S., Doi, H., Iseki, S., Ninomiya, Y., Fujiwara, M., Watanabe, T. and Eto, K. (1993) A mutation in the Pax-6 gene in rat small eye is associated with impaired migration of midbrain crest cells. Nat Genet 3:299-304.
27. Morriss-Kay, G.M. (1993) Postimplantation mammalian embryos. In: Stern, C.D. and Holland, P. (eds) Essential Developmental Biology A Practical Approach. IRL Press, Oxford, pp 55-66.
28. Muhr, J., Andersson, E., Persson, M., Jessell, T.M. and Ericson, J. (2001) Groucho-mediated transcriptional repression establishes progenitor cell pattern and neuronal fate in the ventral neural tube. Cell 104:861-873.
29. Muller, F., Albert, S., Blader, P., Fischer, N., Hallonet, M. and Strahle, U. (2000) Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. Development 127:3889-3897.
30. Muneoka, K., Wanek, N., Treviono, C. and Bryant, S.V. (1990) Exo utero surgery. In: Copp., A.J. and Cockroft, D.L. (eds) Mammalian Postimplantation Embryos: A Practical Approach. IRL, Press, Oxford, pp 41-59.
31. Muramatsu, T., Mizutani, Y., Ohmori, Y. and Okumura, J. (1997) Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem Biophys Res Commun 230:376-380.
32. Nakamura, H. and Funahashi, J. (2001) Introduction of DNA into chick embryos by in ovo electroporation. Methods 24:43-48.
33. Nasevicius, A. and Ekker, S.C. (2000) Effective targeted gene 'knockdown' in zebrafish. Nat Genet 26:216-220.
34. New, D.A.T (1990) Introduction. In: Copp., A.J. and Cockroft, D.L. (eds) Mammalian Postimplantation Embryos: A Practical Approach. IRL, Press, Oxford, pp 1-14.
35. Olsson, M., Campbell, K. and Turnbull, D.H. (1997) Specification of mouse telencephalic and mid-hindbrain progenitors following heterotopic ultrasound-guided embryonic transplantation. Neuron 19:761-772.
36. Osumi, N., Hirota, A., Ohuchi, H., Nakafuku, M., Iimura, T., Kuratani, S., Fujiwara, M., Noji, S. and Eto, K. (1997) Pax-6 is involved in the specification of hindbrain motor neuron subtype. Development 124:2961-2972.
37. Osumi, N. and Inoue, T. (2001) Gene transfer into cultured mammalian embryos by electroporation. Methods 24:35-42.
38. Osumi, N. and Nakafuku, M. (1998) Pax-6 is involved in specification of ventral cell types in the hindbrain. In: Uyemura, K., Kawamura, K. and Yazaki, T. (eds) Neural Development: Keio Univ. Symposia for Life Science and Medicine Vol. 2. Springer-Verlag, Tokyo, pp 1117-1124.
39. Osumi-Yamashita, N., Kuratani, S., Ninomiya, Y., Aoki, K., Iseki, S., Chareonvit, S., Doi, H., Fujiwara, M., Watanabe, T. and Eto, K. (1997) Cranial anomaly of homozygous rSey rat is associated with a defect in the migration pathway of midbrain crest cells. Dev Growth Differ 39:53-67.
40. Papaioannou, V.E. (1990) In utero manipulation. In: Copp, A.J. and Cockroft, D.L. (eds) Mammalian Postimplantation Embryos: A Practical Approach. IRL, Press, Oxford, pp 61-80.
41. Porter, A. (1998) Controlling your losses: conditional gene silencing in mammals. Trends Genet 14:73-79.
42. Puelles, L. and Rubenstein, J.L. (1993) Expression patterns of homeobox and other putative regulatory genes in the embryonic mouse forebrain suggest a neuromeric organization. Trends Neurosci 16:472-479.
43. Saito, T. and Nakatsuji, N. (2001) Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240:237-246.
44. Sakamoto, N., Fukuda, K., Watanuki, K., Sakai, D., Komano, T., Scotting, P.J. and Yasugi, S. (2000) Role for cGATA-5 in transcriptional regulation of the embryonic chicken pepsinogen gene by epithelial-mesenchymal interactions in the developing chicken stomach. Dev Biol 223:103-113.
45. Serbedzija, G.N. Fraser, S. and Bronner-Fraser, M. (1990) Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling. Development 108:605-612.
46. Summerton, J. (1999) Morpholino antisense oligomers: the case for an RNase H independent structural type. Biochim Biophys Acta 1489:141-158.
47. Summerton, S. and Weller, D. (1997) Morpholino antisense oligomers: design, preparation, and properties. Antisense Nucleic Acid Drug Dev 7:187-195.
48. Swartz, M., Eberhart, J., Mastick, G.S. and Krull, C.E. (2001) Sparking New Frontiers: Using in Vivo Electroporation for Genetic Manipulations. Dev Biol 233:13-21.
49. Tabata, H. and Nakajima, K. (2001) Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex. Neuroscience 103:865-872.
50. Takahashi, M. and Osumi, N. (2002) Pax6 regulates specification of ventral neurone subtypes in the hindbrain by establishing progenitor domains. Development 129:1327-1338.
51. Timmer, J., Johnson, J. and Niswander, L. (2001) The use of in ovo electroporation for the rapid analysis of neural-specific murine enhancers. Genesis 29:123-132.
52. Tomioka, N., Osumi, N., Sato, Y., Inoue, T., Nakamura, S., Fujisawa, H. and Hirata, T. (2000) Neocortical origin and tangential migration of guidepost neurons in the lateral olfactory tract. J Neurosci 20:5802-5812.
53. Tucker, R.P. (2001) Abnormal neural crest cell migration after the in vivo knockdown of tenascin-C expression with morpholino antisense oligonucleotides. Dev Dyn 222:115-119.
54. Turnbull, D.H. (2000) Ultrasound backscatter microscopy of mouse embryos. Methods Mol Biol 135:235-243.
55. Wichterle, H., Turnbull, D.H., Nery, S., Fishell, G. and Alvarez-Buylla, A. (2001) In utero fate mapping reveals distinct migratory pathways and fates of neurons born in the mammalian basal forebrain. Development 128:3759-3771.
56. Yamasaki, T., Kawaji, K., Ono, K., Bito, H., Hirano, T., Osumi, N. and Kengaku, M. (2001) Pax6 regulates granule cell polarization during parallel fiber formation in the developing cerebellum. Development 128:3133-3144.
57. Yasuda, K., Momose, T. and Takahashi, Y. (2000) Applications of microelectroporation for studies of chick embryogenesis. Dev Growth Differ 42:203-206.
58. Yasugi, S. and Nakamura, H. (2000) Gene transfer into chicken embryos as an effective system of analysis in developmental biology. Dev Growth Differ 42:195-197.