Effective targeted gene 'knockdown' in zebrafish

Nature Genetics

Volumn 26, Issue 2, 2000, Pages 216-220

  Nasevicius, Aidas ^a   Ekker, Stephen C ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; MORPHOLINE DERIVATIVE;

ARTICLE; CYCLOPIA; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENE TECHNOLOGY; GENETIC DISORDER; HOLOPROSENCEPHALY; NONHUMAN; PORPHYRIA; PRIORITY JOURNAL; TRANSGENE; ZEBRA FISH;

ANIMAL; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; DISEASE MODELS, ANIMAL; FEMALE; GENOME; HUMAN; LUMINESCENT PROTEINS; MORPHOGENESIS; MORPHOLINES; OLIGODEOXYRIBONUCLEOTIDES, ANTISENSE; PORPHYRIA, ERYTHROHEPATIC; PROTEINS; SIGNAL TRANSDUCTION; UROPORPHYRINOGEN DECARBOXYLASE; ZEBRAFISH; ZYGOTE;

EID: 0033780376     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/79951     Document Type: Article

References (30)

1. Summerton, J. Morpholino antisense oligomers: the case for an RNase H-independent structural type. Biochim. Biophys. Acta 1489, 141-158 (1999).
2. Schulte-Merker, S., van Eeden, F.J., Halpern, M.E., Kimmel, C.B. & Nusslein-Volhard, C. no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene. Development 120, 1009-1015 (1994).
3. Schulte-Merker, S., Lee, K.J., McMahon, A.P. & Hammerschmidt, M. The zebrafish organizer requires chordino. Nature 387, 862-863 (1997).
4. Zhang, J., Talbot, W.S. & Schier, A.F. Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation. Cell 92, 241-251 (1998).
5. Lister, J.A., Robertson, C.P., Lepage, T., Johnson, S.L. & Raible, D.W. nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Development 126, 3757-3767 (1999).
6. Parichy, D.M., Rawls, J.F., Pratt, S.J., Whitfield, T.T. & Johnson, S.L. Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development. Development 126, 3425-3436 (1999).
7. Tavernarakis, N., Wang, S.L., Dorovkov, M., Ryazanov, A. & Driscoll, M. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nature Genet. 24, 180-183 (2000).
8. Wang, H., Long, Q., Marty, S.D., Sassa, S. & Lin, S. A zebrafish model for hepatoerythropoietic porphyria. Nature Genet. 20, 239-243 (1998).
9. Krauss, S., Concordet, J.P. & Ingham, P.W. A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. Cell 75, 1431-1444 (1993).
10. Ekker, S.C. et al. Patterning activities of vertebrate hedgehog proteins in the developing eye and brain. Curr. Biol. 5, 944-955 (1995).
11. Summerton, J. & Weller, D. Morpholino antisense oligomers: design, preparation, and properties. Antisense Nucleic Acid Drug Dev. 7, 187-195 (1997).
12. Arora, V. et al. c-Myc antisense limits rat liver regeneration and indicates role for c- Myc in regulating cytochrome P-450 3A activity. J. Pharmacol. Exp. Ther. 292, 921-928 (2000).
13. Qin, G., Taylor, M., Ning, Y.Y., Iversen, P. & Kobzik, L. In vivo evaluation of a morpholino antisense oligomer directed against tumor necrosis factor-α. Antisense Nucleic Acid Drug Dev. 10, 11-16 (2000).
14. Heasman, J., Kofron, M. & Wylie, C. β-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach. Dev. Biol. 222, 124-134 (2000).
15. Fisher, S., Amacher, S.L. & Halpern, M.E. Loss of cerebrum function ventralizes the zebrafish embryo. Development 124, 1301-1311 (1997).
16. Hammerschmidt, M. et al. dino and mercedes, two genes regulating dorsal development in the zebrafish embryo. Development 123, 95-102 (1996).
17. Gritsman, K. et al. The EGF-CFC protein one-eyed pinhead is essential for nodal signaling. Cell 91, 121-132 (1999).
18. Halpern, M.E. et al. Genetic interactions in zebrafish midline development. Dev. Biol., 187, 154-170 (1997).
19. Schier, A.F., Neuhauss, S.C., Heide, K.A., Talbot, W.S. & Driever, W. The one-eyed pinhead gene functions in mesoderm and endoderm formation in zebrafish and interacts with no tail. Development 124, 327-342 (1997).
20. Fekany, K. et al. The zebrafish bozozok locus encodes Dharma, a homeodomain protein essential for induction of gastrula organizer and dorsoanterior embryonic structures. Development 126, 1427-1438 (1999).
21. Koos, D.S. & Ho, R.K. The nieuwkoid/dharma homeobox gene is essential for bmp2b repression in the zebrafish pregastrula. Dev. Biol. 215, 190-207 (1999).
22. Haffter, P. et al. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development 123, 1-36 (1996).
23. Kappas, A., Sassa, S., Galbraith, R.A. & Nordmann, Y. The Metabolic Basis of Inherited Diseases (eds Scriver, C.R., Beaudet, A.L. & Sly, W.S.) 2103-2159 (McGraw-Hill, New York, 1995).
24. Wallis, D.E. & Muenke, M. Molecular mechanisms of holoprosencephaly. Mol. Genet. Metab. 68, 126-138 (1999).
25. Belloni, E. et al. Identification of Sonic hedgehog as a candidate gene responsible for holoprosencephaly. Nature Genet. 14, 353-356 (1996).
26. Roessler, E. et al. Mutations in the human Sonic Hedgehog gene cause holoprosencephaly. Nature Genet. 14, 357-360 (1996).
27. Schauerte, H.E. et al. Sonic hedgehog is not required for the induction of medial floor plate cells in the zebrafish. Development 125, 2983-2993 (1998).
28. Concordet, J.P. et al. Spatial regulation of a zebrafish patched homologue reflects the roles of sonic hedgehog and protein kinase A in neural tube and somite patterning. Development 122, 2835-2846 (1996).
29. Miller-Bertoglio, V., Fisher, S., Sanchez, A., Mullins, M. & Halpern, M.E. Differential regulation of chordin expression domains in zebrafish. Dev. Biol. 192, 537-550 (1998).
30. Sasai, Y. et al. Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Cell 79, 779-790 (1994).