Efficient gene transfer into the embryonic mouse brain using in vivo electroporation

Developmental Biology

Volumn 240, Issue 1, 2001, Pages 237-246

  Saito, Tetsuichiro ^a   Nakatsuji, Norio ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

DNA transfer; DsRed; Electroporation; Embryonic mouse brain; Exo utero; EYFP; Fluorescent protein; Forced gene expression; In utero; In vivo

Indexed keywords

BRAIN PROTEIN; DNA; MESSENGER RNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; CONTROLLED STUDY; DNA VECTOR; ELECTRIC POTENTIAL; ELECTROPORATION; EMBRYO; FEMALE; FLUORESCENCE; GENE EXPRESSION; GENE FUNCTION; GENE TRANSFER; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0035584249     PISSN: 00121606     EISSN: None     Source Type: Journal    
DOI: 10.1006/dbio.2001.0439     Document Type: Article

References (24)

1. Autoradiographic study of cell migrations during histogenesis of cerebral cortex in mouse
2. The migration of neuroblasts in the developing cerebral cortex
3. Retrovirus vectors and their applications in neurobiology
4. Ligand-activated site-specific recombination in mice
5. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter
6. A method for rapid gain-of-function studies in the mouse embryonic nervous system
7. Genome-wide, large-scale production of mutant mice by ENU mutagenesis
8. Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system
9. "Shocking" developments in chick embryology: Electroporation and in ovo gene expression
10. Generation of destabilized green fluorescent protein as a transcription receptor
11. Exo utero surgery
12. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo
13. Efficient selection for high-expression transfectants with a novel eukaryotic vector
14. Ecdyson-inducible gene expression in mammalian cells and transgenic mice
15. A systematic, genome-wide, phenotype-driven mutagenesis program for gene function studies in the mouse
16. Hes1 and Hes5 as Notch effectors in mammalian neuronal differentiation
17. Specification of mouse telencephalic and mid-hindbrain progenitors following heterotopic ultrasound-guided embryonic transplantation
18. Identification of novel paired homeodomain protein related to C. elegans unc-4 as a potential downstream target of MASH1
19. Viral vectors for modulating gene expression in neurons
20. Sparking new frontiers: Using in vivo electroporation for genetic manipulation
21. Efficient in utero gene transfer system to the developing mouse brain using electroporation: Visualization of neuronal migration in the developing cortex
22. Subregion- and cell type-restricted gene knockout in mouse brain
23. Direct gene transfer into mouse muscle in vivo
24. Cortical degeneration in the absence of neurotrophin signaling: Dendritic retraction and neuronal loss after removal of the receptor TrkB