Transient association of Ku with nuclear substrates characterized using fluorescence photobleaching

Journal of Immunology

Volumn 168, Issue 5, 2002, Pages 2348-2355

  Rodgers, William ^a   Jordan, Stephen J ^a   Capra, J Donald ^a  

^a OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; GREEN FLUORESCENT PROTEIN; KU ANTIGEN;

ANTIGEN STRUCTURE; ARTICLE; BLEACHING; CELL TRANSPORT; CONTROLLED STUDY; DIFFUSION; DNA BINDING; DNA REPAIR; DNA STRAND BREAKAGE; EXTRACTION; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; IRRADIATION; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN TRANSPORT;

EID: 0036498909     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: 10.4049/jimmunol.168.5.2348     Document Type: Article

References (49)

1. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
2. Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids
3. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II
4. The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen
5. Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells
6. Ku protein stimulates DNA end joining by mammalian DNA ligases: A direct role for Ku in repair of DNA double-strand breaks
7. The DNA-dependent protein kinase
8. Mechanism of interaction between Ku protein and DNA
9. Analysis of the mechanism of interaction of simian Ku protein with DNA
10. Production and characterization of recombinant human Ku antigen
11. Ku is a general inhibitor of DNA-protein complex formation and transcription
12. Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
13. Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination
14. Ku86 variant expression and function in multiple myeloma cells is associated with increased sensitivity to DNA damage
15. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates
16. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination
17. Growth retardation and leaky SCID phenotype of Ku70-deficient mice
18. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching
19. High mobility of proteins in the mammalian cell nucleus
20. The glucocorticoid receptor: Rapid exchange with regulatory sites in living cells
21. Nucleolar components involved in ribosome biogenesis cycle between the nucleolus and nucleoplasm in interphase cells
22. The transcription coactivator cbp is a dynamic component of the promyelocytic leukemia nuclear body
23. Making genes green: Creating green fluorescent protein (GFP) fusions with blunt-end PCR products
24. Glycolipid-enriched membrane domains are assembled into membrane patches by associating with the actin cytoskeleton
25. Measurement of the β-sheet-forming propensities of amino acids
26. Sequestration of mammalian Rad51-recombination protein into micronuclei
27. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
28. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement
29. Trafficking of endothelial nitric-oxide synthase in living cells: Quantitative evidence supporting the role of palmitoylation as a kinetic trapping mechanism limiting membrane diffusion
30. Action of DNA repair endonuclease ERCC1/XPF in living cells
31. Studying protein dynamics in living cells
32. Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions
33. Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells
34. Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles
35. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
36. Binding of matrix attachment regions to lamin B1
37. The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex
38. Ku is associated with the telomere in mammals
39. Ku acts in a unique way at the mammalian telomere to prevent end joining
40. The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities
41. The DNA-binding protein Hdflp (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae
42. Dimerization and nuclear localization of Ku proteins
43. Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers
44. Sir-Ku-itous routes to make ends meet
45. Compartmentalization of eukaryotic gene expression: Causes and effects
46. Functional compartmentalization of the nucleus
47. Dynamic binding of histone H1 to chromatin in living cells
48. Rapid exchange of histone H1.1 on chromatin in living human cells
49. Half a century of "the nuclear matrix"