High mobility of proteins in the mammalian cell nucleus

Nature

Volumn 404, Issue 6778, 2000, Pages 604-609

  Phair, Robert D ^a   Misteli, Tom ^b  

^a BioInformatics Services   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN;

ANIMAL CELL; ARTICLE; BLEACHING; CELL NUCLEUS; CELL STRAIN BHK; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; KINETICS; NONHUMAN; PRIORITY JOURNAL;

BIOLOGICAL TRANSPORT; CELL LINE; CELL NUCLEUS; CHROMOSOMAL PROTEINS, NON-HISTONE; FLUORESCENCE; HELA CELLS; HIGH MOBILITY GROUP PROTEINS; HUMANS; KINETICS; NUCLEAR PROTEINS; RECOMBINANT FUSION PROTEINS;

ANIMALIA; MAMMALIA;

EID: 0034611785     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35007077     Document Type: Article

References (30)

1. Lamond, A. I. & Earnshaw, W. C. Structure and function in the nucleus. Science 280, 547-553 (1998).
2. Misteli, T. & Spector, D. L. The cellular organization of gene expression. Curr. Opin. Cell Biol. 10, 322-331 (1998).
3. Matera, A. G. Nuclear bodies: multifaceted subdomains of the interchromatin space. Trends Cell Biol. 9, 302-309 (1999).
4. Bustin, M. Regulation of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins. Mol. Cell. Biol. 19, 5237-5246 (1999).
5. Hock, R., Wilde, F., Scheer, U. & Bustin, M. Dynamic relocation of chromosomal protein HMG-17 in the nucleus is dependent on transcriptional activity. EMBO J. 17, 6992-7001 (1998).
6. Ge, H. & Manley, J. L. A protein factor, ASF, controls cell specific alternative splicing of SV40 early premRNA in vitro. Cell 62, 24-34 (1990).
7. Krainer, A. R., Conway, G. C. & Kozak, D. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites. Cell 62, 35-42 (1990).
8. Cáceres, J. F., Misteli, T., Screaton, G., Spector, P. L. & Krainer, A. R. Role of the modular domains of SR-proteins in subnuclear localization and alternative splicing specificity. J. Cell Biol. 138, 225-238 (1997).
9. Scheer, U. & Hock, R. Structure and function of the nucleolus. Curr. Opin. Cell Biol. 11, 385-390 (1999).
10. Olson, M. O. J., Dundre, M. & Szebeni, A. The nucleolus: An old factory with unexpected capabilities. Trends. Cell Biol. (in the press).
11. Ochs, R. L., Lischwe, M. A., Spohn, W. H. & Busch, H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol. Cell 54, 123-133 (1985).
12. Misteli, T., Cáceres, J. F. & Spector, P. I., The dynamics of a pre-mRNA splicing factor in living cells. Nature 387, 523-527 (1997).
13. White, J. & Stelzer, E. Photobleaching GFP reveals protein dynamics inside live cells. Trends Cell Biol. 9, 61-65 (1999).
14. Seksek, O., Biwersi, J. & Verkman, A. S. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus. J. Cell Biol. 138, 131-142 (1997).
15. Yokoe, H. & Meyer, T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nature Biotechnol. 14, 1252-1256 (1996).
16. Houtsmuller, A. B. et al. Action of DNA repair endonuclease FRCCI/XPF in living cells. Science 284, 958-961 (1999).
17. Kanda, T., Sullivan, K. F. & Wahl, G. M. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 26, 377-385 (1998).
18. Foster, D. M. et al. in Proceeding of the Simulation in Health Science Conference 87-90 (Society for Computer Simulation, San Diego, 1994).
19. Bell, B. M., Burke, J. V. & Schumitzky, A. A relative weighting method for estimating parameters and variances in multiple data sets. Computational Statistics and Data Analysis 22, 119-135 (1996).
20. Hanamura, A., Cáceres, J. F., Mayeda, A., Franza, B. A. & Krainer, A. R. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA 4, 430-444 (1998).
21. Politz, J. C., Browne, E. S., Wolf, D. E. & Pederson, T. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl Acad. Sci. USA 95, 6043-6048 (1998).
22. Daneholt, B. Pre-mRNP particles: From gene to nuclear pore. Curr. Biol. 11, R412-R415 (1999).
23. Politz, J. C., Tuft, R. A., Pederson, T. & Singer, R. H. Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells. Curr. Biol. 9, 285-291 (1999).
24. Singh, O. P., Bjorkroth, B., Masich, S., Wieslander, L. & Daneholt, B. The intranuclear movement of Balbiani ring premessenger ribonucleoprotein particles. Exp. Cell Res. 251, 135-146 (1999).
25. Sleeman, J. E., Platani, M., Kreiv, J. P., Lamond, A. I. Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein. Exp. Cell Res. 243, 290-304 (1998).
26. Boudonck, K., Dolan, L. & Shaw, P. J. The movement of coiled bodies visualized in living plant cells by the green fluorescent protein. Mol. Biol. Cell 10, 2297-2307 (1999).
27. Jolly, C., Usson, Y. & Morimoto, R. I. Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules. Prof. Natl Acad. Sci. USA 96, 6769-6774 (1999).
28. Misteli, T. & Spector, D. L. Serine/threonine phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors. Mol. Biol. Cell 7, 1559-1572 (1996).
29. Dingwall, C., Black, S. J., Kearsey, S. E., Cox, L. S. & Laskey, R. A. Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals. EMBO J. 6, 69-74 (1987).
30. Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E. & Webb, W. W. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16, 1055-1069 (1976).