Multiple roles for SR proteins in trans splicing

Molecular and Cellular Biology

Volumn 22, Issue 15, 2002, Pages 5337-5346

  Furuyama, Suzanne ^a   Bruzik, James P ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; PROTEIN; PROTEIN SR; SERINE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; REACTION ANALYSIS; RNA BINDING; SPLICEOSOME; TRANS SPLICING;

ANIMALS; ASCARIS LUMBRICOIDES; CAPSID; CAPSID PROTEINS; CELL LINE; CELL-FREE SYSTEM; MACROMOLECULAR SUBSTANCES; NUCLEAR PROTEINS; NUCLEASE PROTECTION ASSAYS; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RNA SPLICING; RNA, SPLICED LEADER; RNA-BINDING PROTEINS; SPLICEOSOMES;

EID: 0036318192     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.15.5337-5346.2002     Document Type: Article

References (62)

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4. Enhancer-dependent interaction between 5′ and 3′ splice sites in trans
5. Trans splicing involves a novel form of small nuclear ribonucleoprotein particles
6. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
7. Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro
8. Protein phosphatase 1 can modulate alternative 5′ splice site selection in a HeLa splicing extract
9. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors
10. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution
11. Selection of alternative 5′ splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1
12. The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase
13. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3′ splice site
14. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
15. Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation
16. Sorting out the complexity of SR protein functions
17. 65 in enhancer-dependent splicing
18. A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers
19. Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing
20. A serine kinase regulates intracellular localization of splicing factors in the cell cycle
21. Trans splicing of nematode pre-messenger RNA in vitro
22. U small nuclear ribonucleoprotein requirements for nematode cis and trans splicing in vitro
23. The function of multisite splicing enhancers
24. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
25. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
26. Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor
27. Regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins
28. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors
29. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
30. The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes
31. Functional recognition of 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
32. Nuclease protection of RNAs containing site-specific labels: A rapid method for mapping RNA-protein interactions
33. Direct analysis of nematode cis- and trans-spliceosomes: A functional role for U5 snRNA in spliced leader addition trans-splicing and the identification of novel Sm snRNPs
34. Uncovering the role of Ser/Thr protein phosphorylation in nuclear pre-mRNA splicing
35. Ser/Thr-specific protein phosphatases are requires for both catalytic steps of pre-mRNA splicing
36. Regulation of mammalian spliceosome assembly by a protein phosphorylation mechanism
37. The type 2C Ser/Thr phosphatase PP2Cγ is a pre-mRNA splicing factor
38. Trans-splicing
39. The protein kinase Clk/Sty directly modulates SR protein activity: Both hyper- and hypophosphorylation inhibit splicing
40. 3′ splice site recognition in nematode trans-splicing involves enhancer-dependent recruitment of U2 snRNP
41. SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome
42. Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I
43. Developmental regulation of SR protein phosphorylation and activity
44. Regulation of SR protein localization during development
45. SR proteins are required for nematode trans-splicing in vitro
46. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
47. SR proteins are sufficient for exon bridging across an intron
48. Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: Creation of an SRp40-specific splicing enhancer
49. SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain
50. Adenosine phosphorothioates (ATPαS and ATPγS) differentially affect the two steps of mammalian pre-mRNA splicing
51. The C. elegans trans-spliced leader RNA is bound to Sm and has a trimethylguanosine cap
52. Trans-spliced leader RNA exists as small nuclear ribonucleoprotein particles in Caenorhabditis elegans
53. SRPK2: A differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
54. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
55. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing
56. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2
57. Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5′ exon
58. Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae
59. SR proteins: A conserved family of pre-mRNA splicing factors
60. Pre-mRNA splicing in the absence of an SR protein RS domain
61. Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins
62. 35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing