Direct analysis of nematode cis- and trans-spliceosomes: A functional role for U5 snRNA in spliced leader addition trans-splicing and the identification of novel Sm snRNPs

RNA

Volumn 2, Issue 8, 1996, Pages 735-745

  Maroney, Patricia A ^a   Yu, Yi Tao ^a,c   Jankowska, Marzena ^b   Nilsen, Timothy W ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WARSAW   (Poland)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

cis splicing; nematodes; spliceosomes; trans splicing; U snRNAs

Indexed keywords

SIGNAL PEPTIDE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; AUTORADIOGRAPHY; CROSS LINKING; EXON; NEMATODE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; RNA ANALYSIS; RNA SPLICING; SPLICEOSOME;

ANIMALS; ASCARIS LUMBRICOIDES; AUTOANTIGENS; BASE SEQUENCE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RIBONUCLEOPROTEIN, U5 SMALL NUCLEAR; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA SPLICING; RNA, HELMINTH; RNA, MESSENGER; RNA, SMALL NUCLEAR; SPLICEOSOMES;

NEMATODA;

EID: 0029853505     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Abmayr SM, Reed R, Maniatis T. 1988. Identification of a functional mammalian spliceosome containing unspliced pre-mRNA. Proc Natl Acad Sci USA 55:7216-7220.
2. Agabian N. 1990. Trans-splicing of nuclear pre-mRNAs. Cell 61:1157-1160.
3. Baserga SJ, Steitz JA. 1993. The diverse world of small ribonucleoproteins. In: Gesteland RF, Atkins JF, eds. The RNA world. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 359-381.
4. Bruzik JP, Steitz JA. 1990. Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system. Cell 62:889-899.
5. Bruzik JP, van Doren K, Hirsh D, Steitz JA. 1988. Trans-splicing involves a novel form of small ribonucleoprotein particles. Nature 335:559-562.
6. Crispino JD, Blencowe BJ, Sharp PA. 1994. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 265:1866-1869.
7. Denker JA, Maroney PA, Yu YT, Kanost RA, Nilsen TW. 1996. Multiple requirements for nematode spliced leader RNP function in traiis-splicing. RNA 2:746-755.
8. Grabowski PJ, Sharp PA. 1986. Affinity chromatography of splicing complexes: U2, U5, and U4+U6 small nuclear ribonucleoprotein particles in the spliceosome. Science 233:1294-1299.
9. Hall SL, Padgett RA. 1996. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science 271:1716-1718.
10. Hannon GJ, Maroney PA, Denker JA, Nilsen TW. 1990. Trans-splicing of nematode pre-messenger RNA in vitro. Cell 61:1247-1255.
11. Hannon GJ, Maroney PA, Nilsen TW. 1991. U small nuclear ribonucleoprotein requirements for nematode ris- and trans-splicing in vitro. J Biol Chem 266:22792-22695.
12. Hannon GJ, Maroney PA, Yu YT, Hannon GE, Nilsen TW. 1992. Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing. Science 258:1775-1780.
13. Lamm GM, Blencowe BJ, Sproat BS, Irabarren AM, Ryder U, Lamond AI. 1991. Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucleic Acids Res 19:3193-3198.
14. Lamond AI, Konarska PJ, Grabowski PJ, Sharp PA. 1988. Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci USA 85:411-415.
15. Lerner EA, Lerner MR, Janeway CAJ, Steitz JA. 1981. Monoclonal antibodies to nucleic acid-containing cellular constituents: Probes for molecular biology and autoimmune disease. Proc Natl Acad Sci USA 78:2737-2741.
16. Madhani HD, Guthrie C. 1994. Dynamic RNA-RNA interactions in the spliceosome. Annu Rev Genet 28:1-26.