Pre-mRNA splicing in the absence of an SR protein RS domain

Genes and Development

Volumn 14, Issue 24, 2000, Pages 3166-3178

  Zhu, J ^a   Krainer, A R ^a  

^a Watson School of Biological Sciences   (United States)

Author keywords

Pre mRNA splicing; RS domain; SF2 ASF; Splicing enhancer; SR proteins; U2AF

Indexed keywords

MESSENGER RNA PRECURSOR; PROTEIN; SF2 ASF PROTEIN; SR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENHANCER REGION; ENZYME SPECIFICITY; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RNA SPLICING;

BASE SEQUENCE; ENHANCER ELEMENTS (GENETICS); EXONS; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; PEPTIDE FRAGMENTS; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICE SITES; RNA SPLICING; RNA, MESSENGER;

EID: 0034671932     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.189500     Document Type: Article

References (71)

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3. Exonic splicing enhancers: Mechanism of action, diversity and role in human genetic diseases
4. Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains
5. Mammalian pre-mRNA splicing factors
6. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity
7. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
8. Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro
9. hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing
10. RNA splicing specificity determined by the coordinated action of RNA recognition motifs in SR proteins
11. Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing
12. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors
13. hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer
14. Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA
15. Protein phosphorylation plays an essential role in the regulation of alternative splicing and sex determination in Drosophila
16. A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection
17. Selection of alternative 5′ splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1
18. A protein factor ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro
19. A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2
20. Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing
21. Evidence for substrate-specific requirement of the splicing factor U2AF(35) and for its function after polypyrimidine tract recognition by U2AF(65)
22. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors
23. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
24. Binding of the Drosophila sex-lethal gene product to the alternative splice site of transformer primary transcript
25. U1 snRNP-ASF/SF2 interaction and 5′ splice site recognition: Characterization of required elements
26. Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor
27. Regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins
28. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors
29. The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs)
30. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites
31. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
32. Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
33. The structure and function of proteins involved in mammalian pre-mRNA splicing
34. Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles
35. Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development
36. Distinct factor requirements for exonic splicing enhancer function and binding of U2AF to the polypyrimidine tract
37. Exonic splicing enhancer motif recognized by human SC35 under splicing conditions
38. SC35-mediated reconstitution of splicing in U2AF-depleted nuclear extract
39. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
40. Mammalian in vitro splicing assays
41. Preparation of HeLa cell nuclear and cytosolic S100 extracts for in vitro splicing
42. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
43. Inhibition of msl-2 splicing by sex-lethal reveals interaction between U2AF35 and the 3′ splice site AG
44. RNA splicing: What has phosphorylation got to do with it?
45. The protein kinase Clk/Sty directly modulates SR protein activity: Both hyper- and hypophosphorylation inhibit splicing
46. The organization of 3′ splice-site sequences in mammalian introns
47. Mechanisms of fidelity in pre-mRNA splicing
48. A role for exon sequences and splice-site proximity in splice-site selection
49. Exon definition may facilitate splice site selection in RNAs with multiple exons
50. SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome
51. Molecular genetic analysis of the heterodimeric splicing factor U2AF: The RS domain on either the large or small Drosophila subunit is dispensable in vivo
52. Developmental regulation of SR protein phosphorylation and activity
53. Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA
54. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
55. Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: Creation of an SRp40-specific splicing enhancer
56. Modulation of 5′ splice site choice in pre-messenger RNA by two distinct steps
57. The SR protein family: Pleiotropic functions in pre-mRNA splicing
58. Interaction of U2AF65 RS region with pre-mRNA branch point and promotion of base pairing with U2 snRNA
59. SRPK2: A differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
60. Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing
61. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
62. The role of exon sequences in splice site selection
63. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
64. Functional recognition of the 3′ splice site AG by the splicing factor U2AF35
65. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing
66. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2
67. Phosphorylation regulates in vivo interaction and molecular targeting of serine/arginine-rich pre-mRNA splicing factors
68. Biochemical characterization of U2 snRNP auxiliary factor: An essential pre-mRNA splicing factor with a novel intranuclear distribution
69. Both subunits of U2AF recognize the 3′ splice site in Caenorhabditis elegans
70. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing
71. Functional domains of the human splicing factor ASF/SF2