Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation

Molecular and Cellular Biology

Volumn 22, Issue 13, 2002, Pages 4622-4637

  Kim, Young Kyeung ^a   Bourgeois, Cyril F ^a   Isel, Catherine ^a   Churcher, Mark J ^a   Karn, Jonathan ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5,6 DICHLOROBENZIMIDAZOLE RIBOSIDE; CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE INHIBITOR; CYCLIN T1; NUCLEOTIDE; PHOSPHOPROTEIN PHOSPHATASE 1; PYRANOSIDE; RNA POLYMERASE II; TRANSACTIVATOR PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; GENETIC TRANSCRIPTION; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; TRANSCRIPTION INITIATION;

BASE SEQUENCE; CHROMOSOMAL PROTEINS, NON-HISTONE; CYCLIN-DEPENDENT KINASE 9; CYCLIN-DEPENDENT KINASES; DICHLORORIBOFURANOSYLBENZIMIDAZOLE; ENZYME INHIBITORS; GENE PRODUCTS, TAT; HELA CELLS; HIV-1; HUMANS; ISOPROPYL THIOGALACTOSIDE; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHORYLATION; REPETITIVE SEQUENCES, AMINO ACID; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSCRIPTIONAL ELONGATION FACTORS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0036275982     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.13.4622-4637.2002     Document Type: Article

References (74)

1. TFIIH is negatively regulated by cdk8-containing mediator complexes
2. Requirement for TFIIH kinase activity in transcription by RNA polymerase II
3. Transcription elongation through DNA arrest sites: A multistep process involving both RNA polymerase II subunit RPB9 and TFIIS
4. Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat
5. Conformation of the RNA polymerase II C-terminal domain: Circular dichroism of long and short fragments
6. Spt-5 cooperates with Tat by preventing premature RNA release at terminator sequences
7. Flavopiridol inhibits p-TEFb and blocks HIV-1 replication
8. Specific interaction of Tat with the human but not rodent pTEFb complex mediates the species-specific Tat activation of HIV-1 transcription
9. Tat activates human immunodeficiency virus type 1 transcriptional elongation independent of TFIIH kinase
10. A protein phosphatase functions to recycle RNA polymerase II
11. Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, p37
12. The RNA element encoded by the trans-activation-responsive region of human immunodeficiency virus type 1 is functional when displaced downstream of the start of transcription
13. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution
14. The HIV trans-activator Tat binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II
15. Topological localization of the carboxyl-terminal domain of RNA polymerase II in the initiation complex
16. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation
17. Capping, splicing, and 3′ processing are independently stimulated by RNA polymerase II: Different functions for different segments of the CTD
18. Relief of two built-in autoinhibitory mechanisms in p-TEFb is required for the assembly of a multicomponent transcription elongation complex at the HIV-1 promoter
19. Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T
20. CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 Tat-P-TEFb complex to TAR RNA
21. HIV-1 Tat interacts with cyclin T1 to direct P-TEFb CTD kinase complex to TAR RNA
22. The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein
23. Structural basis of transcription: An RNA polymerase II elongation complex at 3.3 Å resolution
24. Viral transactivators E1A and VP16 interact with a large complex that is associated with CTD kinase activity and contains CDK8
25. Human immunodeficiency virus type 1 trans-activator protein Tat, stimulates transcriptional read-through of distal terminator sequences in vitro
26. DNA-binding studies of XSPTSPSZ, derivatives of the intercalating heptad repeat of RNA polymerase II
27. Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: Candidate for a Tat cofactor
28. RNA polymerase II and the integration of nuclear events
29. Direct evidence that HIV-1 Tat activates the Tat-associated kinase (TAK) during transcriptional elongation
30. Domains in the SPT5 protein that modulate its transcriptional regulatory properties
31. Cyclin T domains involved in complex formation with Tat and TAR RNA are critical for Tat-activation
32. Anti-termination of transcription within the long terminal repeat of HIV-1 by Tat gene product
33. Tackling Tat
34. Transfer of Tat and release of TAR RNA during the activation of the human immunodeficiency virus type-1 transcription elongation complex
35. Analysis of signals for transcription termination by purified RNA polymerase II
36. Positive transcription elongation factor b phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase
37. Different phosphorylated forms of RNA polymerase II, and associated mRNA processing factors during transcription
38. TFIIS enhances transcriptional elongation through an artificial arrest site in vivo
39. Role of the human and murine cyclin T proteins in regulating HIV-1 Tat-activation
40. T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT 1 complex in vivo and regulates its CTD kinase activity
41. p-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
42. Regulation of carboxyl-terminal domain phosphatase by HIV-1 Tat protein
43. C-terminal domain phosphatase sensitivity of RNA polymerase II in early elongation complexes on the HIV-1 and adenovirus 2 major late templates
44. Principles of CDK regulation
45. 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
46. Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain
47. A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro
48. Yeast carboxyl-terminal domain kinase I positively and negatively regulates RNA polymerase II carboxyl-terminal domain phosphorylation
49. Mechanisms of cyclin-dependent kinase regulation: Structures of cdks, their cyclin activators, and Cip and INK4 inhibitors
50. Tat-associated kinase (P-TEF-b): A component of transcription preinitiation and elongation complexes
51. DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
52. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
53. Three RNA polymerase II CTD kinases display distinct substrate preferences
54. A highly purified RNA polymerase II elongation control system
55. Phosphorylation in transcription: The CTD and more
56. Dynamic assocation of capping enzymes with transcribing RNA polymerase II
57. Purified elongation factor SII is sufficient to promote read-through by purified RNA polymerase II at specific termination sites in the human histone H3.3 gene
58. The heptad repeat in the largest subunit of RNA polymerase II binds by intercalating into DNA
59. Interactions between equine cyclin T1, Tat, and TAR are disrupted by a leucine-to-valine substitution found in human cyclin T1
60. Tat transactivation: A model for the regulation of eukaryotic transcriptional elongation
61. FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between p-TEFb and TFIIH
62. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro
63. DSIF, a novel transcription elongation factor that regulates RNA polymerse II processivity, is composed of human Spt4 and Spt5 homologs
64. Characterization of mediator complexes from HeLa cell nuclear extract
65. A novel cdk9-associated c-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop specific binding to TAR RNA
66. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation
67. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation
68. Structure and function of the human transcription elongation factor DSIF
69. The 7SK small nuclear RNA inhibits CDK9/cyclin T1 kinase to control transcription
70. Recycling of the general transcription factors during RNA polymerase II transcription
71. HIV-1 TAR RNA enhances the interaction between Tat and cyclin T1
72. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription
73. TFIIH inhibits CDK9 phosphorylation during human immunodeficiency virus type 1 transcription
74. Transcription elongation factor p-TEFb is required for HIV-1 Tat trans-activation in vitro