Characterization of mediator complexes from HeLa cell nuclear extract

Molecular and Cellular Biology

Volumn 21, Issue 14, 2001, Pages 4604-4613

  Wang, G ^a   Cantin, G T ^a   Stevens, J L ^a   Berk, A J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; CELL PROTEIN; CYCLIN DEPENDENT KINASE 8; CYCLINE; PROTEIN SUBUNIT; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; GEL FILTRATION CHROMATOGRAPHY; HELA CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN PURIFICATION;

CELL EXTRACTS; CELL FRACTIONATION; CELL NUCLEUS; CHROMATOGRAPHY, GEL; CYCLIN-DEPENDENT KINASES; CYCLINS; HELA CELLS; HUMANS; PROTEIN-SERINE-THREONINE KINASES; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

MAMMALIA; MURINAE; SACCHAROMYCES CEREVISIAE;

EID: 0034956192     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.14.4604-4613.2001     Document Type: Article

References (42)

1. TFIIH is negatively regulated by cdk8-containing mediator complexes
2. Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein
3. Glucose repression in yeast
4. Binding of liganded vitamin D receptor to the vitamin D receptor interacting protein coactivator complex induces interaction with RNA polymerase II holoenzyme
5. A human RNA polymerase II complex containing factors that modify chromatin structure
6. Eukaryotic gene transcription with purified components
7. Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex
8. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
9. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae
10. Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation
11. Dissecting the regulatory circuitry of a eukaryotic genome
12. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators
13. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways
14. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II
15. Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: Little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure
16. An RNA polymerase II holoenzyme responsive to activators
17. A kinase-cyclin pair in the RNA polymerase II holoenzyme
18. Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes
19. A human RNA polymerase II complex associated with SRB and DNA-repair proteins
20. The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC anal acts synergistically with other PCs
21. Transcriptional regulation through Mediator-like coactivators in yeast and metazoan cells
22. Corepressor required for adenovirus E1B 55,000-molecular-weight protein repression of basal transcription
23. RNA polymerase II holoenzymes and subcomplexes
24. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain
25. Composite co-activator ARC mediates chromatin-directed transcriptional activation
26. Factors associated with the mammalian RNA polymerase II holoenzyme
27. A mammalian RNA polymerase II holoenzyme containing all components required for promoter-specific transcription initiation
28. Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators
29. The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes
30. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex
31. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1
32. NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription
33. Biochemical mechanism of transcriptional activation by GAL4-VP16
34. General requirement for RNA polymerase II holoenzymes in vivo
35. Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody
36. Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex
37. Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice
38. RNA polymerase II holoenzyme contains SWI/SNF regulators involved in chromatin remodeling
39. NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities
40. Analysis of the NuRD subunits reveals a histone deacetylase core complex anal a connection with DNA methylation
41. SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex
42. Holo-TFIID supports transcriptional stimulation by diverse activators anal from a TATA-less promoter