Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana

Structure

Volumn 10, Issue 1, 2002, Pages 93-103

  Wilmouth, Rupert C ^a   Turnbull, Jonathan J ^a   Welford, Richard W D ^a   Clifton, Ian J ^a   Prescott, Andrea G ^b   Schofield, Christopher J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

2 oxoglutarate; Anthocyanidin synthase; Anthocyanins; Flavonoid biosynthesis; Leucoanthocyanidin dioxygenase; Quercetin

Indexed keywords

ANTHOCYANIDIN SYNTHASE; ANTHOCYANIN; FLAVANOID; METAL; OXYGEN; OXYGENASE; QUERCETIN; SUCCINIC ACID; TAXIFOLIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME ENGINEERING; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROXYLATION; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; OXIDATION; PRIORITY JOURNAL; STEREOCHEMISTRY; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BINDING SITES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; OXYGENASES; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0036151286     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00695-5     Document Type: Article

References (52)

1. A biochemical survey of some Mendelian factors for flower colour
2. The formation of anthocyanin in plants
3. Advances in flavonoid research since 1992
4. Biosynthesis of flavonoids
5. Biosynthesis of flavonoids
6. a of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase
7. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
8. Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase
9. Purification and characterization of flavone synthase I, a 2-oxoglutarate dependent desaturase
10. Molecular cloning, sequence analysis, and in vitro expression of flavanone 3β-hydroxylase from Petunia hybrida
11. Cloning and expression of flavonol synthase from Petunia hybrida
12. Direct evidence for anthocyanidin synthase as a 2-oxoglutarate dependent oxygenase: Molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens
13. Are anthocyanidins the immediate products of anthocyanidin synthase?
14. Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes
15. 2 sensing
16. 2-regulated prolyl hydroxylation
17. C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation
18. The iron(II), 2-oxoacid-dependent dioxygenases and their role in metabolism
19. Evidence for oxidation at C-3 of the flavonoid C-ring during anthocyanin biosynthesis
20. Structure of a cephalosporin synthase
21. Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase
22. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation
23. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)
24. Studies on the active site of deacetoxycephalosporin C synthase
25. Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes
26. The Alrestatin double-decker: Binding of two inhibitor molecules to human aldose reductase reveals a new specificity determinant
27. A kinetic study of thymine 7-hydroxylase from Neurospora crassa
28. Substrate binding to the alpha-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation
29. Biosynthesis
30. Leucoanthocyanidins as intermediates in anthocyanidin biosynthesis in flowers of Matthiola incana R-Br
31. Role of the nonheme Fe(II) center in the biosynthesis of the plant hormone ethylene
32. Steady-state kinetic mechanism of recombinant avocado ACC oxidase: Initial velocity and inhibitor studies
33. High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base
34. Mendel's dwarfing gene: cDNAs from the Le alleles and the function of the expressed proteins
35. Mendel's stem length gene (Le) encodes a gibberellin 3β-hydroxylase
36. Genetic analysis of gibberellin biosynthesis
37. Purification, crystallization and preliminary X-ray diffraction of anthocyanidin synthase from Arabidopsis thaliana
38. The CCP4 suite: Programs for protein crystallography
39. Automated structure solution for MIR and MAD
40. Reciprocal-space solvent flattening
41. Maximum likelihood density modification
42. Free R-value: A novel statistical quantity for assessing the accuracy of crystal structures
43. Improved methods for building protein models in electron density maps and the location of errors in these models
44. Crystallography and NMR system: A new software suite for macromolecular structure determination
45. AMoRe - An automated package for molecular replacement
46. MolScript: A program to produce both detailed and schematic plots of protein structures
47. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
48. Raster3D version 2.0: A program for photorealistic molecular graphics
49. ALSCRIPT - A tool to format multiple sequence alignments