Enumeration of the simian virus 40 early region elements necessary for human cell transformation

Molecular and Cellular Biology

Volumn 22, Issue 7, 2002, Pages 2111-2123

  Hahn, William C ^a   Dessain, Scott K ^a   Brooks, Mary W ^a   King, Jessie E ^a   Elenbaas, Brian ^a   Sabatini, David M ^a   DeCaprio, James A ^a   Weinberg, Robert A ^a  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOPROTEIN PHOSPHATASE 2A; PROTEIN P53; TELOMERASE; VIRUS LARGE T ANTIGEN;

ANCHORAGE INDEPENDENT GROWTH; ARTICLE; BREAST EPITHELIUM; CANCER CELL; CELL TRANSFORMATION; CONTROLLED STUDY; EPITHELIUM CELL; FIBROBLAST; GENE MUTATION; HUMAN; HUMAN CELL; KIDNEY CELL; MALIGNANT TRANSFORMATION; ONCOGENE H RAS; PRIORITY JOURNAL; RETINOBLASTOMA; SIMIAN VIRUS 40;

ANTIGENS, VIRAL, TUMOR; CELL AGING; CELL DIVISION; CELL LINE; CELL TRANSFORMATION, NEOPLASTIC; DNA-BINDING PROTEINS; FIBROBLASTS; HUMANS; ONCOGENE PROTEIN P21(RAS); RETINOBLASTOMA PROTEIN; SIGNAL TRANSDUCTION; SIMIAN VIRUS 40; TELOMERASE; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

SIMIAE; SIMIAN VIRUS 40;

EID: 0036118368     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.7.2111-2123.2002     Document Type: Article

References (95)

1. Cellular transformation by SV40 large T antigen: Interaction with host proteins
2. SV40 small t antigen enhances the transformation activity of limiting concentrations of SV40 large T antigen
3. Human mesothelial cells are unusually susceptible to simian virus 40-mediated transformation and asbestos cocarcinogenicity
4. Extension of life-span by introduction of telomerase into normal human cells
5. Low frequency of alterations of the alpha (PPP2R1A) and beta (PPP2R1B) isoforms of the subunit A of the serine-threonine phosphatase 2A in human neoplasms
6. Requirement for the simian virus 40 small tumor antigen in tumorigenesis in transgenic mice
7. The three transforming regions of SV40 T antigen are required for immortalization of primary, mouse embryo fibroblasts
8. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
9. The SV40 small t antigen is essential for the morphological transformation of human fibroblasts
10. Human keratinocytes that express hTERT and also evade a p16ink4a-enforced lifespan limit become immortal while retaining normal growth and differentiation characteristics
11. Cellular proteins that are targetted by DNA tumor viruses for transformation
12. Human breast cancer cells generated by oncogenic transformation of primary, mammary epithelial cells
13. Functional expression of human PP2Ac in yeast permits the identification of novel C-terminal and dominant-negative mutant forms
14. Structure and function of simian virus 40 large tumor antigen
15. Viral transactivating proteins
16. The ability of human papillomavirus E6 proteins to target p53 for degradation in vivo correlates with their ability to abrogate actinomycin D-induced growth arrest
17. The complexity of p53 modulation: Emerging patterns from divergent signals
18. pRB-dependent, J domain-independent function of simian virus 40 large T antigen in override of p53 growth suppression
19. Delayed embryonic lethality in mice lacking protein phosphatase 2A catalytic subunit Cα
20. Creation of human tumor cells with defined genetic elements
21. Telomerase activity is sufficient to allow transformed cells to escape from crisis
22. Monoclonal antibodies specific for simian virus 40 tumor antigens
23. p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts
24. Regulation of retinoblastoma protein functions by ectopic expression of human cyclins
25. Rescue of cells from ras oncogene-induced growth arrest by a second, complementing, oncogene
26. Mechanism of HPV E6 proteins in cellular transformation
27. Protein phosphatase 2A: A highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling
28. Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens
29. In vitro mutagenesis of a putative DNA binding domain of SV40 large-T
30. Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF
31. Association of p53 binding and immortalization of primary C57BL/6 mouse embryo fibroblasts by using simian virus 40 T-antigen mutants bearing internal overlapping deletion mutations
32. Specific association of human telomerase activity with immortal cells and cancer
33. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP)
34. Hypervariable ultra-long telomeres in mice
35. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells
36. New HPV E6 binding proteins: Dangerous liaisons?
37. The p53 protein and its interactions with the oncogene products of the small DNA tumor viruses
38. p53, the cellular gatekeeper for growth and division
39. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells
40. Identification of structural elements involved in the interaction of simian virus 40 small tumor antigen with protein phosphatase 2A
41. Malignant transformation of human fibroblasts by oncogene transfection or carcinogen treatment
42. Activated Ha-ras can cooperate with defective simian virus 40 in the transformation of nonestablished rat embryo fibroblasts
43. Regulation of protein kinase cascades by protein phosphatase 2A
44. Simian virus 40 small tumor antigen and an amino-terminal domain of large tumor antigen share a common transforming function
45. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase
46. Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
47. Interactions of HPV E6 and E7 oncoproteins with tumour suppressor gene products
48. Mutations which affect the inhibition of protein phosphatase 2A by simian virus 40 small-t antigen in vitro decrease viral transformation
49. Fibroblast immortality is a prerequisite for transformation by EJ c-HA-ras oncogene
50. Induction of immortality is an early event in malignant transformation of mammalian cells by carcinogens
51. Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A
52. The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens
53. Mutants with changes within or near a hydrophobic region of simian virus 40 large tumor antigen are defective for binding cellular protein p53
54. Escape from premature senescence is not sufficient for oncogenic transformation by Ras
55. Role of T antigen interactions with p53 in tumorigenesis
56. Mutational analysis of simian virus 40 T antigen: Isolation and characterization of mutants with deletions in the T-antigen gene
57. Characterization of SV40 T antigen polypeptides synthesized in vivo and in vitro
58. Developmental and tissue-specific regulation of mouse telomerase and telomere length
59. A genetically tractable model of human glioma formation
60. Role of small t antigen in the acute transforming activity of SV40
61. The role of the SV40 ST antigen in cell growth promotion and transformation
62. Senescence as a mode of tumor suppression
63. Resistance of human cells to tumorigenesis induced by cloned transforming genes
64. Signaling by integrins: Implications for tumorigenesis
65. Can the ends justify the means?: Telomeres and the mechanisms of replicative senescence and immortalization in mammalian cells
66. Role of the retinoblastoma protein in the pathogenesis of human cancer
67. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16ink4a
68. 1 arrest checkpoint in response to DNA damage
69. Identification of a minimal transforming domain of p53: Negative dominance through abrogation of sequence-specific DNA binding
70. A role for both RB and p53 in the regulation of human cellular senescence
71. Defining the molecular mechanisms of human cell immortalization
72. Construction and analysis of viable deletion mutants of simian virus 40
73. Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells
74. The interaction of SV40 small tumor antigen with protein phosphatase 2A stimulates the map kinase pathway and induces cell proliferation
75. The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain
76. The large tumor antigen of simian virus 40 encodes at least two distinct transforming functions
77. Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells
78. Inactivation of pRB-related proteins p130 and p107 mediated by the J domain of simian virus 40 large T antigen
79. Dissociation of Rb-binding and anchorage-independent growth from immortalization and tumorigenicity using SV40 mutants producing N-terminally truncated large T antigens
80. Uncoupling IL-2 signals that regulate T cell proliferation, survival, and Fas-mediated activation-induced cell death
81. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span
82. Nucleotide sequence deletions within the coding region for small-t antigen of simian virus 40
83. Alterations of the PPP2R1B gene in human lung and colon cancer
84. Expression of catalytically active telomerase does not prevent premature senescence caused by overexpression of oncogenic Ha-Ras in normal human fibroblasts
85. A p16INK4a-insensitive CDK4 mutant targeted by cytotoxic T lymphocytes in a human melanoma
86. Telomere dynamics in cancer progression and prevention: Fundamental differences in human and mouse telomere biology
87. Human endothelial cell life extension by telomerase expression
88. Control of protein phosphatase 2A by simian virus 40 small-t antigen
89. Role of pRb-related proteins in simian virus 40 large-T-antigen-mediated transformation Mol
90. The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins
91. Independent expression of the transforming amino-terminal domain of SV40 large T antigen from an alternatively spliced third SV40 early mRNA
92. Transformation of a continuous rat embryo fibroblast cell line requires three separate domains of simian virus 40 large T antigen
93. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening
94. Derivation of human tumor cells in vitro without widespread genomic instability