Adenoviral-mediated, high-level, cell-specific transgene expression: A SYN1-WPRE cassette mediates increased transgene expression with no loss of neuron specificity

Molecular Therapy

Volumn 5, Issue 5, 2002, Pages 509-516

  Glover, Colin P J ^a   Bienemann, Alison S ^a   Heywood, Darren J ^a   Cosgrave, A Siobhan ^a   Uney, James B ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Adenovirus; Cell targeting; Gene therapy; Neuronal; SYN1 promoter; WPRE

Indexed keywords

ADENOVIRUS VECTOR; GREEN FLUORESCENT PROTEIN; REGULATOR PROTEIN; SYNAPSIN I;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BASE PAIRING; BRAIN TISSUE; CELL CULTURE; CELL SPECIFICITY; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; DENTATE GYRUS; GENE CASSETTE; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENE THERAPY; GLIA; HEPATITIS VIRUS; HIPPOCAMPUS; HUMAN; HUMAN CELL; IN VIVO STUDY; MALE; NERVE CELL; NONHUMAN; PROMOTER REGION; RAT; TRANSCRIPTION REGULATION; TRANSGENE;

ADENOVIRIDAE; ANIMALIA; MARMOTA MONAX; WOODCHUCK HEPATITIS B VIRUS;

EID: 0036109543     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2002.0588     Document Type: Article

References (46)

1. Gene therapy for acute diseases
2. Gene therapy in the CNS
3. Towards gene therapy for the central nervous system
4. An adenovirus vector for gene transfer into neurons and glia in the brain
5. Long-term gene therapy in the CNS: Reversal of hypothalamic diabetes insipidus in the Brattleboro rat by using an adenovirus expressing arginine vasopressin
6. Adeno-associated virus vectors: Activity and applications in the CNS
7. Differential and persistent expression patterns of CNS gene transfer by an adeno-associated virus (AAV) vector
8. Long-term gene expression and phenotypic correction using adeno-associated virus vectors in the mammalian brain
9. Stable gene transfer to the nervous system using a non-primate lentiviral vector
10. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
11. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector
12. Gene delivery and gene therapy with herpes simplex virus-based vectors
13. Methods for construction of adenovirus vectors
14. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5
15. Targeting of adenovirus to endothelial cells by a bispecific single-chain diabody directed against the adenovirus fiber knob domain and human endoglin (CD105)
16. Targeting a recombinant adenovirus vector to HCC cells using a bifunctional Fab-antibody conjugate
17. Ablating adenovirus type 5 fiber-CAR binding and HI loop insertion of the SIGYPLP peptide generate an endothelial cell-selective adenovirus
18. Targeting of high-capacity adenoviral vectors
19. Efficient gene transfer and long-term expression in neurons using a recombinant adenovirus with a neuron-specific promoter
20. Targeting expression of hsp70i to discrete neuronal populations using the Lmo-1 promoter: Assessment of the neuroprotective effects of hsp70i in vivo and in vitro
21. Neuron-specific expression of therapeutic proteins: Evaluation of different cellular promoters in recombinant adenoviral vectors
22. A common silencer element in the SCG10 and type II Na+ channel genes binds a factor present in nonneuronal cells but not in neuronal cells
23. The neuron-restrictive silencer factor (NRSF): A coordinate repressor of multiple neuron-specific genes
24. Brain-derived neurotrophic factor expression in vivo is under the control of neuron-restrictive silencer element
25. Neuron-specific gene expression of synapsin I. Major role of a negative regulatory mechanism
26. REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons
27. Neuron-restrictive silencer elements mediate neuron specificity of adenoviral gene expression
28. Targeting of tetracycline-regulatable transgene expression specifically to neuronal and glial cell populations using adenoviral vectors
29. Peripheral infection with adenovirus causes unexpected long-term brain inflammation in animals injected intracranially with first-generation, but not with high-capacity, adenovirus vectors: Toward realistic therapy for chronic diseases
30. Immune responses to adenovirus vectors in the nervous system
31. Comparison of the human versus murine cytomegalovirus immediate early gene promoters for transgene expression by adenoviral vectors
32. Strong promoters are the key to highly efficient, noninflammatory and noncytotoxic adenoviral-mediated transgene delivery into the brain in vivo
33. Acute direct adenoviral vector cytotoxicity and chronic, but not acute, inflammatory responses correlate with decreased vector-mediated transgene expression in the brain
34. Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element
35. Enhanced expression of transgenes from adeno-associated virus vectors with the woodchuck hepatitis virus posttranscriptional regulatory element: Implications for gene therapy
36. High-level erythroid-specific gene expression in primary human and murine hematopoietic cells with self-inactivating lentiviral vectors
37. Influence of promoter and WHV post-transcriptional regulatory element on AAV-mediated transgene expression in the rat brain
38. Lentiviral vectors for enhanced gene expression in human hematopoietic cells
39. Context dependence of different modules for posttranscriptional enhancement of gene expression from retroviral vectors
40. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors
41. Assessing viral gene therapy in neuroendocrine models
42. Regulatable adenoviral technology in behavioural neuroscience
43. Persistent transgene expression in the hypothalamus following stereotaxic delivery of a recombinant adenovirus: Suppression of the immune response with cyclosporin
44. Switching transgene expression in the brain using an adenoviral tetracycline-regulatable system
45. Overexpression of hsp70i facilitates reactivation of intracellular proteins in neurones and protects them from denaturing stress
46. A simple method for organotypic cultures of nervous tissue